Single-point isotope measurements in blood cells and plasma to estimate the time since diet switches

1. Understanding ecological phenomena often requires an accurate assessment of the timing of events. To estimate the time since a diet shift in animals without knowledge on the isotope ratios of either the old or the new diet, isotope ratio measurements in two different tissues (e.g. blood plasma and blood cells) at a single point in time can be used. For this ‘isotopic-clock’ principle, we present here a mathematical model that yields an analytical and easily calculated outcome.

2. Compared with a previously published model, our model assumes the isotopic difference between the old and new diets to be constant if multiple measurements are taken on the same subject at different points in time. Furthermore, to estimate the time since diet switch, no knowledge of the isotopic signature of tissues under the old diet, but only under the new diet is required.

3. The two models are compared using three calibration data sets including a novel one based on a diet shift experiment in a shorebird (red knot Calidris canutus); sensitivity analyses were conducted. The two models behaved differently and each may prove rather unsatisfactory depending on the system under investigation. A single-tissue model, requiring knowledge of both the old and new diets, generally behaved quite reliably.

4. As blood (cells) and plasma are particularly useful tissues for isotopic-clock research, we trawled the literature on turnover rates in whole blood, cells and plasma. Unfortunately, turnover rate predictions using allometric relations are too unreliable to be used directly in isotopic-clock calculations.

5. We advocate that before applying the isotopic-clock methodology, the propagation of error in the ‘time-since-diet-shift’ estimation is carefully assessed for the system under scrutiny using a sensitivity analysis as proposed here.

An adaptive orientation misalignment calibration method for shoulder movements using inertial sensors : a feasibility study

Qualitative assessment of the progress in physical rehabilitation largely depends on accurate measurement of the range of movements and other kinematic parameters. In clinical practice, wearable inertial sensors have proved to be a potential candidate for such measurements, over the traditional marker based optical systems due to cost and space considerations. The accuracy of wearable sensors have a significant dependence on the initial orientation calibration and the assumption that the sensor will not slip or move with respect to the attached limb. This article introduces a novel calibration algorithm to correct initial orientation misalignment, as well as to track and correct subsequent alignment errors progressively throughout the experiment. The theoretical assertions are validated through controlled experiments with simulated accelerometer and gyroscope measurements.