The role of micro-organisms (staphylococcus aureus and Candida Albicans) in the pathogenesis of breast pain and infection in lactation women : study protocol

Background: The CASTLE (Candida and Staphylococcus Transmission: Longitudinal Evaluation) study will investigate the micro-organisms involved in the development of mastitis and “breast thrush” among breastfeeding women. To date, the organism(s) associated with the development of breast thrush have not been identified. The CASTLE study will also investigate the impact of physical health problems and breastfeeding problems on maternal psychological health in the early postpartum period.

Methods/Design: The CASTLE study is a longitudinal descriptive study designed to investigate the role of Staphylococcus spp (species) and Candida spp in breast pain and infection among lactating women, and to describe the transmission dynamics of S. aureus and Candida spp between mother and infant. The relationship between breastfeeding and postpartum health problems as well as maternal psychological well-being is also being investigated. A prospective cohort of four hundred nulliparous women who are at least thirty six weeks gestation pregnant are being recruited from two hospitals in Melbourne, Australia (November 2009 to June 2011). At recruitment, nasal, nipple (both breasts) and vaginal swabs are taken and participants complete a questionnaire asking about previous known staphylococcal and candidal infections. Following the birth, participants are followed-up six times: in hospital and then at home weekly until four weeks postpartum. Participants complete a questionnaire at each time points to collect information about breastfeeding problems and postpartum health problems. Nasal and nipple swabs and breast milk samples are collected from the mother. Oral and nasal swabs are collected from the baby. A telephone interview is conducted at eight weeks postpartum to collect information about postpartum health problems and breastfeeding problems, such as mastitis and nipple and breast pain.

Discussion: This study is the first longitudinal study of the role of both staphylococcal and candidal colonisation in breast infections and will help to resolve the current controversy about which is the primary organism in the condition known as breast thrush. This study will also document transmission dynamics of S. aureus and Candida spp between mother and infant. In addition, CASTLE will investigate the impact of common maternal physical health symptoms and the effect of breastfeeding problems on maternal psychological well-being.

Measuring the filtration efficiency of the Continuous Plankton Recorder

The Continuous Plankton Recorder (CPR) survey has analysed over 160 000 plankton samples taken in the North East Atlantic over the last 60 years. However, flowmeters have not been routinely fitted to the recorders and there has been the implicit assumption that a CPR sample represents the organisms filtered from 3 m3 of sea water. We describe an electromagnetic flowmeter designed for the CPR and present results which show that on occasions the filtered volume per sample varied significantly from this value.

Inter- and intra-beach thermal variation for Green Turtle nests on Ascension Island, South Atlantic

Nest temperatures for green turtles (Chelonia mydas) nesting on Ascension Island, South Atlantic (7°57'S 14°22'W), were examined. Temperature probes were placed into nests on two beaches, Long Beach (26 nests) and North East Bay (8 nests). Within these beaches there was relatively little thermal variation (SD of nest temperature was 0.32°C for Long Beach and 0.30°C for North East Bay). To examine inter-beach thermal variation temperature probes were buried at 55 cm on 12 beaches. Inter-beach thermal variation was large and was related to the beach albedo with the darkest beach (albedo, 016) being 4.2°C warmer than the lightest coloured beach (albedo, 0.73).

Nest site selection by sea turtles

The distribution of 38 nests of loggerhead turtles (Caretta caretta) on beaches on Sanibel and Captiva islands, south-western Florida (26°26'N 82°16'W), and of 70 first digging attempts by green turtles (Chelonia mydas) on Ascension Island (7°57'S 14°22'W), was quantified. For loggerhead turtles on Sanibel and Captiva, nests were clumped close to the border between the open sand and the supra-littoral vegetation that backed the beaches. This spatial pattern of nests was closely reproduced by assuming simply that turtles crawled a random distance above the most recent high water line prior to digging. In contrast, green turtles on Ascension Island clumped their first digging attempts on the uneven beach above the springs high water line, crawling up to 80 m to reach this beach zone.

Psychometric validation of the Self-Care Inventory-Revised (SCI-R) in UK adults with type 2 diabetes using data from the AT.LANTUS follow-on study.

Background:
Achieving optimal outcomes in type 2 diabetes (T2DM) involves several demanding self-care behaviours, e.g. managing diet, activity, medications, monitoring glucose levels, footcare. The Self-Care Inventory-Revised (SCI-R) is valid for use in people with T2DM in the US. Our aim was to determine its suitability for use in the UK.