Bioinformatics based structural characterization of glucose dehydrogenase (gdh) gene and growth promoting activity of Leclercia sp. QAU-66

Glucose dehydrogenase (GDH; EC 1.1. 5.2) is the member of quinoproteins group that use the redox cofactor pyrroloquinoline quinoine, calcium ions and glucose as substrate for its activity. In present study, Leclercia sp. QAU-66, isolated from rhizosphere of Vigna mungo, was characterized for phosphate solubilization and the role of GDH in plant growth promotion of Phaseolus vulgaris. The strain QAU-66 had ability to solubilize phosphorus and significantly (p ≤ 0.05) promoted the shoot and root lengths of Phaseolus vulgaris. The structural determination of GDH protein was carried out using bioinformatics tools like Pfam, InterProScan, I-TASSER and COFACTOR. These tools predicted the structural based functional homology of pyrroloquinoline quinone domains in GDH. GDH of Leclercia sp. QAU-66 is one of the main factor that involved in plant growth promotion and provides a solid background for further research in plant growth promoting activities.

Study of KJ-66 micro gas turbine compressor: steady and unsteady Reynolds-averaged Navier-Stokes approach

Numerical study on the compressor stage of a KJ-66 micro gas turbine was conducted in this paper through both steady and unsteady Reynolds-averaged Navier–Stokes. The study was conducted for the numerical prediction of micro gas turbine compressor performance at various operation conditions, with special attention given to the transient flow behaviors during compressor operation. The numerical results showed reasonable agreements with experimental data while providing predictions for the charting of compressor performance map at various operation speeds. The simulation results indicated that the increase of operation speed from 80 k r/min to 117 k r/min would leads to an increased peak total pressure ratio from 1.54 to 1.96, while decreasing the peak adiabatic efficiency from 0.73 to 0.55. This paper also provided discussion on details of transient flow field within the compressor stage as well as demonstrated the smooth flow transition through rotor–stator interactions.