The Plasmodium translocon of exported proteins (PTEX) component thioredoxin-2 is important for maintaining normal blood-stage growth

Plasmodium parasites remodel their vertebrate host cells by translocating hundreds of proteins across an encasing membrane into the host cell cytosol via a putative export machinery termed PTEX. Previously PTEX150, HSP101 and EXP2 have been shown to be bona fide members of PTEX.

Here we validate that PTEX88 and TRX2 are also genuine members of PTEX and provide evidence that expression of PTEX components are also expressed in early gametocytes, mosquito and liver stages, consistent with observations that protein export is not restricted to asexual stages. Although amenable to genetic tagging, HSP101, PTEX150, EXP2 and PTEX88 could not be genetically deleted in Plasmodium berghei, in keeping with the obligatory role this complex is postulated to have in maintaining normal blood-stage growth.

In contrast, the putative thioredoxin-like protein TRX2 could be deleted, with knockout parasites displaying reduced grow-rates, both in vivo and in vitro, and reduced capacity to cause severe disease in a cerebral malaria model. Thus, while not essential for parasite survival, TRX2 may help to optimize PTEX activity. Importantly, the generation of TRX2 knockout parasites that display altered phenotypes provides a much-needed tool to dissect PTEX function.

Raining blood and money

A fictocritical piece based on a fire in New York.
A girl jumps from the ninth floor of the Asch Building. People on the sidewalk are screaming for her not to jump. But she has to jump. As she stands on the ledge, her back against the open window, the fire is burning the clothes off her back. She has her head bent forward so her hair doesn't catch alight as she waves a handkerchief at the crowd. The windows on the floor beneath her start to explode. Tongues of flame lick at her feet. So she jumps. It's a definite jump, as she bends her knees before she leaps over the edge. It's not a graceful jump. Her arms begin to flail as she struggles to stay upright. There are gasps from the crowd, a few screams. Some people turn away. Others are transfixed, watching her as she falls. Suddenly, her dress catches on a hook jutting out of wall below and she is suspended in the air, mid-fall. But the ladders still can't reach her and so it is just a cruel pause in her inevitable death. She hangs there like a ragdoll until her dress burns itself free from her body and she resumes her fall. She lands on the pavement on the west - ward side of New York University building. Thud-dead...

Iron status and dietary iron intake of female blood donors

Background
The aim was to assess iron status and dietary iron intake in a sample of premenopausal female regular and new blood donors.

Study design and methods
Premenopausal women blood donors were invited to participate. Blood samples were analyzed for serum ferritin and hemoglobin. An iron checklist assessed dietary iron intake. Donors were classified as regular donors or new donors.

Results
Twenty-one new donors (mean [SD] age, 28.6 [6.0] years; body mass index [BMI], 25.6 [4.5] kg/m2) and 172 regular donors (mean age, 29.4 [5.5] years; BMI, 24.7 [3.8] kg/m2) participated. Fifty percent of regular donors and 24% of new donors had depleted iron stores (serum ferritin <15 mg/L; difference p = 0.036). Dietary iron intake was higher in regular donors (mean [SE], 12.6 [0.7] mg/day) compared to new donors (9.9 [0.4] mg/day; p = 0.006). Eighty-five percent of regular donors and 79% of new donors met the estimated average requirement for iron.

Conclusions
Despite the fact that most of these donors had an adequate dietary iron intake, more than half of the blood donors had depleted iron stores. Increasing dietary iron intake through supplements and/or dietary means is expected to be necessary to maintain adequate iron status in this group.

African culturally and linguistically diverse communities’ blood donation intentions in Australia: integrating knowledge into the theory of planned behavior

Background 

Cauldron of blood

Book review of John Kinsella's collection of fiction, Tide, 2013

“Giving Dracula the keys to the blood bank? Interrogating the Fifth Crown Casino Licensing Regulatory Review

Drawing on narrative analysis, this paper analyses the 2013 Fifth Regulatory Review of the license of an Australian casino as a case study focused on the framing and articulation of ‘responsible gambling’ (RG) in the Review. Part 1 sets out the policy and regulatory context for the licensing review of Melbourne’s Crown Casino. Part 2 overviews the structure/content of the Review; the key messages of the Reviewers’ narrative and its main recommendations. In reflecting on the Review in Part 3, analysis focuses on the investigation and recommendations regarding Responsible Gambling, which has gained recent policy priority. The analysis interrogates the Review’s findings, narratives, processes and evidentiary base in relation to how it presents and assesses casino performance on RG. In doing so, it focuses on the Victorian Commission for Gambling and Liquor Regulation’s Review’s framing of RG; sources of evidence drawn on by the Review; an assessment of the casino’s loyalty club feature ‘Play Safe’, as an RG measure; the Review’s assessment of casino performance on RG and its Code of Conduct in particular; and the Review’s framing of RG recommendations. It concludes with reflections on governance issues raised by the Review, the need for more focus on the neglected area of regulatory licensing and enforcement (OECD, 2011; 2012; OECD & European Commission, 2009) and the need for independent regulatory reviews that address conflicts of interest on the part of both Government and the Regulator.

Blood, sweat, and tears documenting Thai citizenship

Thailand (Siam) has transformed ancient methods of keepint track of subjects, and adopted modern legislative principles using documentary evidence to discriminate between citizens and outsiders. In the process, it has shaped a complex hierarchical structure with differentiated layers of citizenship, where some groups exist beyond any legal space. At the same time, Thailand has evolved from a society where subjects paid tribute to sovereigns, into democratic polity where entitlement is determined through identity documentation.