Cystic fibrosis in children of the eastern Arabian peninsula : a clinical, spatial and genetic study

Aim: The aim of this thesis is to describe the process by which the inherited disease, cystic fibrosis, (CF) was recognised as an important clinical entity in the United Arab Emirates (UAE) and the Sultanate of Oman (Oman). It examines the clinical presentation of the first patients and assesses their degree of severity. Further, it describes the first studies carried out to determine the underlying CF mutations associated with the disease in the UAE and Oman. An estimate is offered of the birth frequency of the condition. Overall, the cultural, geographical and historical aspect of the societies in which the disease occurs is stressed. Methods: An initial literature search was carried out using Medline of any literature pertaining to the Arab World and CF. this was read and classified into the relevance to Arabs in general, the Middle East and then specifically the Arab (Persian) Gulf societies. Thereafter, a clinic was established at Tawam Hospital, Al Ain, UAE, for children presenting With chronic respiratory disease that could serve as a national referral centre. It was run by the Author as a service of the Paediatric Department of the UAE University Medical School. I sent a letter to every Paediatrician working in the UAE informing them of our clinic and offering our services for the diagnosis and management of chronic respiratory disease in children. This was based on the author's experience as a respiratory paediatrician in Australia and New Zealand and as the Professor of Paediatrics in the UAE. No such service then existed in the UAE. Funding was sought to establish a research programme and develop a molecular genetics laboratory in the UAE Medical School. A series of successful research applications provided the grants to commence the investigations. Once a small number of children had been identified as having CF from those referred to the respiratory clinic, the initial project was to assess and report their clinical presentation. Following this an early start was made on the identification of the mutations responsible. Once these were established an attempt was made to estimate the frequency of the condition at birth. Additional clinical studies revolved around assessing the severity of the condition that was associated with the main mutations that were identified. A clinical comparison was made with those with the mutation AF508 and the other main mutation, despite the obvious limitation of small numbers then available. Radiological assessment was made to evaluate the progression of the disease. The final aspect of the study was to assess patients from Oman and compare their findings and mutations with the neighbouring UAE. Based on information gained hypotheses are proposed regarding the spread of the gene mutation by population drift. Thesis outline: A literature review is presented in the form of a critique on the disease and a resume of the relevant aspects of the genetics of CF. Additionally, facts about the two countries' geography and history are presented. Finally, knowledge about CF mutations and population origins from other areas is presented. The second main section deals with the clinical features of the disorder as it presents in the UAE. Molecular findings are then presented and details of the common mutation found in Bedouin Arabs. Hypotheses are then presented based on the information gathered. Results: CF is not a rare disease in the Arab children of the UAE and Oman. These findings refute previous reports of CF being a rare or non-existent disease in Arabs. The condition presents with a severe clinical picture, with early colonisation of the respiratory tract with staphylococcus, haemophilus and pseudomonas organisms, even with conventional CF management practices in place. The CF mutation S549R is prevalent in Arabs of Bedouin stock, while AF508 is found in those of Baluch origin. The former may be descendants of Arabs who left southern Arabia and travelled to the Trucial Coast at the time of the destruction of the great dam at Marib. The origins of this mutation may lie in the area that corresponds to the modern Republic of Yemen. The latter groups are descendants of those who came originally from Baluchistan. It is hypothesised also that the ancestral home of the AF508 mutation may be in the geographical area now known as Baluchistan, that spans three separate modern political territories. The evidence presented supports the concept that the S549R mutation may be associated with a severe, if not the severest, clinical pattern recognised. It equates with that seen with the homozygous AF508 genotype. The absence of an additional mutation in the promoter region accounts for the different clinical pattern seen in previously described patients. Conclusions: There needs to be a major awareness of the presence of CF as a severe clinical disease in the children of the Gulf States. The clinical presentation and findings support the concept of under recognition of the disease. Climatic conditions put the children at special risk of hyponatraemia and electrolyte imbalance. The absence of surviving adults with the disease suggests premature deaths have occurred, but the high fertility rates have maintained the gene pool for this recessive disorder.

Kurunuavanua: Fijian bodies and the economy of war

In Kurunavanua (the trembling of the earth) Bolatagici investigates representations of Pacific Island masculinity, Fiji and the economy of war. Her ongoing research is concerned with neo-colonial encounters between the US, Australia, Britain and Pacific Island nations. This exhibition is informed by research into the exploitation of the Pacific by comparing the participation of Fijian soldiers in the nuclear tests at Christmas Island in the 1950s to the current recruitment of Fijians to work for Private Security Military Companies in the Middle East.

The Great East Window of York Minster

This inquiry into the meaning behind the images of this vast stained glass window has uncovered a complex theology of the Mass; replies to Lollard 'heretics'; and has identified threats to the power of the Church at this critical time in English history at the beginning of the fifteenth century.

Representing Fijian bodies and the economy of war

This paper traces representations of the black body, which I have related to contemporary masculine identities in Fiji. I have linked historical representations to what I perceive as the ongoing commodification of the Fijian body and argue that opportunities that have arisen from conflict in the Middle East have had a significant impact on employment opportunities for Fijians. I have framed the discussion around a description and analysis of my own photographic and installation artworks produced between 2007 and 2009.

In the Syrian taste : crusader churches in the Latin East as architectural expressions of orthodoxy

This paper explores how the architectural expression of orthodoxy in the Eastern churches was transferred to Europe before the Crusades and then reinforced through the Crusaders’ adoption of the triple-apsed east end “in the Syrian Taste” in the Holy Land. Previously, I have shown how it can be deduced from the archaeological remains of churches from the 4th-6th C that early church architecture was influenced by the theological ideas of the
period. It is proposed that the Eastern orthodox approach to church
architecture as adopted by the Crusaders paralleled the evolution of medieval theology in Europe and can be seen as its legitimate expression.

Student researchers in the middle : using visual images to make sense of inclusive education

Using ‘visual narrative’ theoretically and practically, this paper explores issues of inclusive education, during a period of curriculum reform and renewal in Australia. In Australia, the middle years of schooling, Years 5 to 9, are well researched and known as a period when students disengage with learning and participation in schooling. Research in the middle years affirms the importance of engaging with ‘student voice’. In this special edition, we are aiming to highlight how the use of visual imagery can be a rich source of understanding, illustrating students’ self-knowledge of schooling. Methodologically we refer to our research approach as ‘visual narrative’. Other writers in this edition use the term ‘photo voice’. For researchers it is important to highlight the differing orientations that ‘visual narrative’ and ‘photo voice’ signify. The terms are not mutually exclusive but highlight differing research possibilities and emphasis. Our argument, through the use of visual narrative produced by middle years’ students, is that visual texts open out some innovative possibilities for understanding inclusive education and supporting new relationships with our research community. Such approaches are not new; however, in a field such as special education that purports to support marginalised groups, liberatory research methods are under-represented. This paper aims to open out these discussions and provide a way forwards for teachers and researchers interested in breaking apart why it is that inclusive education remains a never-ending struggle.

Intercultural harmony and understanding in the city of Whittlesea

The City of Whittlesea is one of the most ethnically diverse urban areas in Melbourne that attracts settlers, often humanitarian migrants from countries in the Horn of Africa and the Middle East. With settlers arriving from a broader range of countries than ever before, increasing ethnic as well as ethno-religious diversity presents opportunities for local government to address intercultural harmony and understanding but also significant challenges. This paper reports the findings of fieldwork conducted in 2009 among residents focusing on attitudes towards ethnic diversity and evaluations of the capacity of local government to promote intercultural harmony and understanding. The results suggest that if local government is to be inclusive and gain the confidence and trust of residents necessary to foster empowering partnerships, political spaces that facilitate interactions between long-term residents, new residents, elected leaders and council officers must be facilitated. Such initiatives will contribute to strengthening programs and policies being developed by local government that aim to address discrimination experienced by ethnic minorities and encourage greater acceptance of cultural diversity among the broader community in ways that move beyond measurable outcomes.

Student views of computer based-mathematics in the middle years : does gender make a difference?

How the use of computers in mathematics classrooms was viewed by students in two middle years mathematics classrooms was the focus of the research described in this paper. The primary data sources consisted of questionnaires, classroom observations supported by videotaping of mathematics lessons, and interviews with two girls and two boys from each class. Thus both qualitative and quantitative methods were used. Girls viewed the computer-based lessons less favourably than did boys. In general, the boys were likely to believe that computers contributed to their experiencing pleasure in these lessons, and to making mathematics more relevant to them. Girls were typically more concerned about whether computers facilitated learning and enabled success in mathematics. The attitudes of students to computer-based mathematics were related to their views of computers.

Development and characterisation of 20 microsatellite loci isolated from the large bent-wing bat, Miniopterus schreibersii (Chiroptera: Miniopteridae) and their cross-taxa utility in the family Miniopteridae

The large bent-wing bat, Miniopterus schreibersii (Kuhl 1819), has a long history of taxonomic uncertainty and many populations are known to be in a state of decline. Microsatellite loci were developed for the taxonomic and population genetic assessment of the Australian complex of this species. Of the 33 primer sets designed for this research, seven (21%) were deemed suitably polymorphic for population-level analyses of the Australian taxa, with five (71%) of these loci revealing moderate to high levels of polymorphism (PIC = 0.56 to 0.91). The cross-taxa utility of the M. schreibersii microsatellite markers was assessed in the microbat (Chiroptera) family Miniopteridae. Sub-species and species covering the Miniopteridae’s global distribution (with the exception of the Middle East) were selected, numbering 25 taxa in total. Amplification was successful for 26 loci, of which 20 (77%) were polymorphic. High cross-taxa utility of markers was observed with amplification achieved for all taxa for between four (20%) and 20 (100%) loci, and polymorphism was considered moderate to high (PIC = 0.47–0.91) for 12 (60%) of these loci. The high cross-taxa utility of the microsatellites reported herein reveal versatile and cost-effective molecular markers, contributing an important genetic resource for the research and conservation of Miniopteridae species worldwide.

On the growth and reproductive biology of asp, Aspius vorax, population from the middle reaches of Euphrates river

Endemic asp, Aspius vorax, from the middle section of the Euphrates River flowing through eastern Syria were studied to determine the main characteristics of their population structure, morphological parameters and reproductive biology. Samples ranged between 0+ and 4+ years of age and were dominated by 2+ years old group. Total length (TL) ranged between 19 and 70 cm corresponding with 46 to 2824.5 g weight, respectively. Fish growth has isometric pattern and the overall sex ratio was unbiased. Seasonal changes in the condition factor were related with the water temperature as well as the spawning season. Annual cycle of gonadosomatic index (GSI) readings indicated that spawning season occur around March when fish longer than 36 cm can mate. Average pre-spawning GSI was greater in individuals older than 2 years. Meanwhile, female fecundity was highly related to TL and weight. These findings did not always concur with previous observations from other asp populations, mainly in southern and northern Mesopotamia. Our results highlighted basic biological aspects of the local population and indicated differences between populations which can assist in fisheries management, conservation and commercial culture of the investigated species.