Altered fast- and slow-twitch muscle fibre characteristics in female mice with a (S248F) knock-in mutation of the brain neuronal nicotinic acetylcholine receptor

We generated a mouse line with a missense mutation (S248F) in the gene (CHRNA4) encoding the α4 subunit of neuronal nicotinic acetylcholine receptor (nAChR). Mutant mice demonstrate brief nicotine induced dystonia that resembles the clinical events seen in patients with the same mutation. Drug-induced dystonia is more pronounced in female mice, thus our aim was to determine if the S248F mutation changed the properties of fast- and slow-twitch muscle fibres from female mutant mice. Reverse transcriptase-PCR confirmed CHRNA4 gene expression in the brain but not skeletal muscles in normal and mutant mice. Ca²⁺ and Sr²⁺ force activation curves were obtained using skinned muscle fibres prepared from slow-twitch (soleus) and fast-twitch (EDL) muscles. Two significant results were found: (1) the (pCa₅₀ - pSr₅₀) value from EDL fibres was smaller in mutant mice than in wild type (1.01 vs. 1.30), (2) the percentage force produced at pSr 5.5 was larger in mutants than in wild type (5.76 vs. 0.24%). Both results indicate a shift to slow-twitch characteristics in the mutant. This conclusion is supported by the identification of the myosin heavy chain (MHC) isoforms. Mutant EDL fibres expressed MHC I (usually only found in slow-twitch fibres) as well as MHC IIa. Despite the lack of spontaneous dystonic events, our findings suggest that mutant mice may be having subclinical events or the mutation results in a chronic alteration to muscle neural input.

Fibre production by beef cows

Cattle grow and shed fibre which assists them adapt to seasonal changes in the environment. In the absence of cattle fibre production data for southern Australia, Angus, Hereford, Simmental and Limousin cows and crosses between these breeds grazing perennial pastures at Hamilton, Victoria were sampled in late winter. The fibre-growing area on the sides of cattle was measured, fibre sampled at the mid-side site and the sampling area determined. Fibre was tested for fibre diameter distribution, clean washing yield and fibre length measured. Cows were 3-7 years of age, liveweights were 412-712 kg and the mean fibre-growing area was 2.2 m². This produced an average 682 g of total fibre (range 3461-175 g). The mean fibre diameter of all fibres was 51.7 μm (range 43-62 μm) and 18% of fibres were 36 μm (range 6-39%). The clean washing yield was 92.4% (range 87.4-95.8%). Fibre length averaged 21 mm. Increasing the age, liveweight and condition score of cows and increasing weight of clean fibre were associated with significant increases in mean fibre diameter. Breed of cattle did not affect fibre production (P > 0.1) but did affect mean fibre diameter (P < 0.05). The quantity of fibre production indicates potential for low value textile production. The high level of total fibre production, twice that of an earlier report, and fibre shedding from cattle suggests that white fibre-producing animals such as Merino sheep, Angora and cashmere goats and alpaca should avoid using cattle-handling facilities, particularly in the month before shearing.

High strain rate tensile properties of rapidly cured woven carbon fibre composites

Tensile tests at high speeds corresponding to automotive crash events were conducted to understand the dynamic properties of rapidly cured woven carbon fiber composites. The High Strain Rate (HSR) experiments were conducted on a servo-hydraulic machine at constant velocities up to a maximum of 25 m/s (82 ft/s). Results from HSR tests were compared with the static results to determine the rate sensitivity of the composite. A high speed camera was used to capture the failure at HSR. The tensile properties of rapidly cured laminate were compared to oven cured laminate to justify its productivity while maintaining the desired properties. The methodology used to achieve constant velocity during HSR tests is discussed in detail. The specimen geometry was specially designed to suit the test rig and to achieve high speeds during tests. All the specimens failed with linear elasticity until sudden brittle fracture. The Scanning Electron Microscopy (SEM) images of the fracture zone were used to identify the failure modes observed at static and high strain rates.

Properties of a carbon-fibre composite modified by electrospun poly (vinylidene fluoride)

The interlaminar toughening of a carbon-fibre reinforced composite by incorporation of electrospun polyvinylidene fluoride (PVDF) nanofibrous membranes was explored in this work. The nanofibres were electrospun directly onto commercial pre-impregnated carbon fibre materials under optimised conditions and PVDF was found to primarily crystallise in its β phase polymorphic form. There is strong evidence from DMTA analysis to suggest that a partial miscibility between the amorphous phases of the PVDF nanofibres and the epoxy exists. The improved plastic deformation at the crack tip after inclusion of the nanofibres was directly translated to a 57% increase in the mode II interlaminar fracture toughness (in-plane shear failure). Conversely, the fracture toughness in mode I (opening failure) was slightly lower than the reference by approximately 20%, and the results were interpreted from the complex micromechanisms of failure arising from the changes in polymorphism of the PVDF.

The indentation behaviour of carbon fibre composite tubes-experiments & modelling

Metallic tubes have been extensively studied for their crashworthiness as they closely resemble automotive crash rails. Recently, the demand to produce lighter weight, yet safer vehicles has led to the need to understand the crash behaviour of novel materials, such as fibre reinforced polymer composites, metallic foams and sandwich structures. This paper discusses the static indentation response of Carbon Fibre Reinforced Polymer (CFRP) tubes. The side impact on a CFRP tube involves various failure mechanisms. This paper highlights these mechanisms and compares the energy absorption of CFRP tubes with similar Aluminium tubes. The response of the CFRP tubes during bending was modelled using ABAQUS finite element software with a composite fabric material model. The material inputs were given based on standard tension and compression test results and the in-plane damage was defined based on cyclic shear tests. The failure modes and energy absorption observed during the tests were well represented by the finite element model.

Rapid fibre diameter measurement in aqueous solutions with OFDA 2000

In this paper, a new technique has been developed to enable fibre diameters to be measured in an aqueous environment using the OFDA 2000 instrument. The existing OFDA instrument has only been used to measure diameters of animal fibres under dry conditions. This new technique was utilised to assess the effects of pH and temperature on the diameters of merino wool fibres in aqueous environments. Significant changes in fibre diameter under aqueous conditions were found as a function of pH and temperature. Wool fibre diameters were at a minimum close to the wool isoelectric point (pH 4.8) and increased at both lower and higher pHs. Swelling of merino wool fibres was observed to increase linearly by around 15% as the temperature rose from ambient to 70°C.

Protein fibre powder

Soft viscoelastic fibres, which are very difficult to grind, can be processed to produce ultrafine particles. This work has created knowledge about new applications of these natural structural proteins. The high reactivity generated through creation of large surface area has been used to design advanced devices and applications. For example particles have been studied for separation of harmful ions from waste water and applied to develop porous composite materials to grow bones to repair critical bone defects.

Enhanced oxygen permeation through perovskite hollow fibre membranes by methane activation

Hollow fibre membranes of mixed conducting perovskite La_0.6Sr_0.4Co_0.2Fe_0.8O₃ (LSCF) were prepared via the combined phase inversion and sintering technique. The fibres were tested for air separation with a home-made reactor under the oxygen partial pressure gradient generated by the air/He streams. Some fibres were in situ activated by introducing methane in the He sweeping gas at high temperatures. The activated membranes with new morphology were created by transforming the inner densified surface layer to a porous structure. Compared to the original membranes, the activated gave appreciable higher oxygen fluxes. At 800 °C, the oxygen fluxes were increased by a factor of 10 after activation was carried out at 1000 °C for 1 h.

High performance BaBiScCo hollow fibre membranes for oxygen transport

Here we report the production of novel high performance BaBi_0.05Sc_0.1Co_0.85O_3-3 (BaBiScCo) hollow fibres delivering oxygen fluxes of 11.4 ml cm^-2 min^-1 at 950 °C. The doping of bismuth, a highly ionic conductor, at the B-site of a barium based perovskite overcame oxygen ionic transport limitations even at temperatures as low as 600 °C.

Ultrasonic scouring of wool and its effects on fibre breakage during carding

Ultrasonics has shown the potential to reduce the cost and environmental impact of textile processing. This work investigates the impact of ultrasonic scouring on fibre entanglement caused during the scouring process. Levels of fibre entanglement were quantified by measuring fibre length using OFDA4000 after carding. A significant reduction in fibre entanglement after ultrasonic scouring was observed and this was due to a reduced fibre migration in the wash bath when compared with the mechanical agitation seen in conventional scouring process. Fibre cuticle scale damage resulting from the ultrasonic irradiation may also have contributed to the reduction in fibre entanglement. A reduced level of fibre entanglement from ultrasonic wool scouring leads to a reduction in fibre breakage during carding.