Robust nonlinear adaptive backstepping excitation controller design for rejecting external disturbances in multimachine power systems

This paper proposes a new approach to design a robust adaptive backstepping excitation controller for multimachine power systems in order to reject external disturbances. The parameters which significantly affect the stability of power systems (also called stability sensitive parameters) are considered as unknown and the external disturbances are incorporated into the power system model. The proposed excitation controller is designed in such a way that it is adaptive to the unknown parameters and robust to external disturbances. The stability sensitive parameters are estimated through the adaptation laws and the convergences of these adaptation laws are obtained through the negative semi-definiteness of control Lyapunov functions (CLFs). The proposed controller not only provides robustness property against external disturbances but also overcomes the over-parameterization problem of stability sensitive parameters which usually appears in some conventional adaptive methods. Finally, the performance of the proposed controller is tested on a two-area four machine 11-bus power system by considering external disturbances under different scenarios and is compared to that of an existing nonlinear adaptive backstepping controller. Simulation results illustrate the robustness of the proposed controller over an existing one in terms of rejecting external disturbances.

Identification, characterisation and expression analysis of natural killer receptor genes in chlamydia pecorum infected koalas (phascolarctos cinereus)

BACKGROUND: Koalas (Phascolarctos cinereus), an iconic Australian marsupial, are being heavily impacted by the spread of Chlamydia pecorum, an obligate intracellular bacterial pathogen. Koalas vary in their response to this pathogen, with some showing no symptoms, while others suffer severe symptoms leading to infertility, blindness or death. Little is known about the pathology of this disease and the immune response against it in this host. Studies have demonstrated that natural killer (NK) cells, key components of the innate immune system, are involved in the immune response to chlamydial infections in humans. These cells can directly lyse cells infected by intracellular pathogens and their ability to recognise these infected cells is mediated through NK receptors on their surface. These are encoded in two regions of the genome, the leukocyte receptor complex (LRC) and the natural killer complex (NKC). These two families evolve rapidly and different repertoires of genes, which have evolved by gene duplication, are seen in different species. METHODS: In this study we aimed to characterise genes belonging to the NK receptor clusters in the koala by searching available koala transcriptomes using a combination of search methods. We developed a qPCR assay to quantify relative expression of four genes, two encoded within the NK receptor cluster (CLEC1B, CLEC4E) and two known to play a role in NK response to Chalmydia in humans (NCR3, PRF1). RESULTS: We found that the NK receptor repertoire of the koala closely resembles that of the Tasmanian devil, with minimal genes in the NKC, but with lineage specific expansions in the LRC. Additional genes important for NK cell activity, NCR3 and PRF1, were also identified and characterised. In a preliminary study to investigate whether these genes are involved in the koala immune response to infection by its chlamydial pathogen, C. pecorum, we investigated the expression of four genes in koalas with active chlamydia infection, those with past infection and those without infection using qPCR. This analysis revealed that one of these four, CLEC4E, may be upregulated in response to chlamydia infection. CONCLUSION: We have characterised genes of the NKC and LRC in koalas and have discovered evidence that one of these genes may be upregulated in koalas with chlamydia, suggesting that these receptors may play a role in the immune response of koalas to chlamydia infection.

Analytical thermal study on nonlinear fundamental heat transfer cases using a novel computational technique

In this paper a novel computational technique called Parameterized Perturbation Method (PPM) is used to obtain the solutions of nonlinear fundamental heat conduction equations. Three well known problems in the area of heat transfer are addressed to be solved. An analytical investigation is carried out for: (a) the temperature distribution in a fin with a temperature-dependent thermal conductivity, (b) the cooling of the lumped system with variable specific heat, and (c) the temperature distribution of a convective-radiative fin. The validity of the results of PPM solution was verified via comparison with numerical results obtained using a fourth order Runge-Kutta method. These comparisons revealed that PPM is a powerful approach for solving these problems. Also, the results showed that the main attributions of this method are very straightforward calculations and low computational burden compared to previous analytical and numerical approaches.

Characterisation of minimalist co-assembled fluorenylmethyloxycarbonyl self-assembling peptide systems for presentation of multiple bioactive peptides

The nanofibrillar structures that underpin self-assembling peptide (SAP) hydrogels offer great potential for the development of finely tuned cellular microenvironments suitable for tissue engineering. However, biofunctionalisation without disruption of the assembly remains a key issue. SAPS present the peptide sequence within their structure, and studies to date have typically focused on including a single biological motif, resulting in chemically and biologically homogenous scaffolds. This limits the utility of these systems, as they cannot effectively mimic the complexity of the multicomponent extracellular matrix (ECM). In this work, we demonstrate the first successful co-assembly of two biologically active SAPs to form a coassembled scaffold of distinct two-component nanofibrils, and demonstrate that this approach is more bioactive than either of the individual systems alone. Here, we use two bioinspired SAPs from two key ECM proteins: Fmoc-FRGDF containing the RGD sequence from fibronectin and Fmoc-DIKVAV containing the IKVAV sequence from laminin. Our results demonstrate that these SAPs are able to co-assemble to form stable hybrid nanofibres containing dual epitopes. Comparison of the co-assembled SAP system to the individual SAP hydrogels and to a mixed system (composed of the two hydrogels mixed together post-assembly) demonstrates its superior stable, transparent, shear-thinning hydrogels at biological pH, ideal characteristics for tissue engineering applications. Importantly, we show that only the coassembled hydrogel is able to induce in vitro multinucleate myotube formation with C2C12 cells. This work illustrates the importance of tissue engineering scaffold functionalisation and the need to develop increasingly advanced multicomponent systems for effective ECM mimicry.

STATEMENT OF SIGNIFICANCE: Successful control of stem cell fate in tissue engineering applications requires the use of sophisticated scaffolds that deliver biological signals to guide growth and differentiation. The complexity of such processes necessitates the presentation of multiple signals in order to effectively mimic the native extracellular matrix (ECM). Here, we establish the use of two biofunctional, minimalist self-assembling peptides (SAPs) to construct the first co-assembled SAP scaffold. Our work characterises this construct, demonstrating that the physical, chemical, and biological properties of the peptides are maintained during the co-assembly process. Importantly, the coassembled system demonstrates superior biological performance relative to the individual SAPs, highlighting the importance of complex ECM mimicry. This work has important implications for future tissue engineering studies.

Transient stability enhancement of multimachine power systems using nonlinear observer-based excitation controller

This paper presents an approach to design a nonlinear observer-based excitation controller for multimachine power systems to enhance the transient stability. The controller is designed based on the partial feedback linearization of a nonlinear power system model which transforms the model into a reducedorder linear one with an autonomous dynamical part. Then a linear state feedback stabilizing controller is designed for the reduced-order linear power system model using optimal control theory which enhances the stability of the entire system. The states of the feedback stabilizing controller are obtained from the nonlinear observer and the performance of this observer-based controller is independent of the operating points of power systems. The performance of the proposed observer-based controller is compared to that of an exact feedback linearizing observer-based controller and a partial feedback linearizing controller without observer under different operating conditions.

Nonlinear adaptive backstepping controller design for controlling bidirectional power flow of BESSs in DC microgrids

In this paper, a nonlinear adaptive backstepping controlleris designed to control the bidirectional power flow (charging/discharging) of battery energy storage systems (BESSs) in a DCmicrogrid under different operating conditions. The controller isdesigned in such a manner that the BESSs can store the excess energyfrom the renewable energy sources (RESs) in a DC microgrid aftersatisfying the load demand and also feeding back the stored energyto the load when RESs are not sufficient. The proposed controller isalso designed to maintain a constant voltage at the DC bus, whereall components of DC microgrids are connected, while controllingthe power flow of BESSs. This paper considers solar photovoltaic(PV) systems as the RES whereas a diesel generator equipped witha rectifier is used as a backup supply to maintain the continuity ofpower supply in the case of emergency situations. The controller isdesigned recursively based on the Lyapunov control theory whereall parameters within the model of BESSs are assumed to beunknown. These unknown parameters are then estimated throughthe adaptation laws and whose stability is ensured by formulatingsuitable control Lyapunov functions (CLFs) at different stages ofthe design process. Moreover, a scheme is also presented to monitorthe state of charge (SOC) of the BESS. Finally, the performanceof the proposed controller is verified on a test DC microgrid undervarious operating conditions. The proposed controller ensures the DCbus voltage regulation within the acceptable limits under differentoperating conditions.