High atmospheric temperatures and 'ambient incubation' drive embryonic development and lead to earlier hatching in a passerine bird

Tropical and subtropical species typically experience relatively high atmospheric temperatures during reproduction, and are subject to climate-related challenges that are largely unexplored, relative to more extensive work conducted in temperate regions. We studied the effects of high atmospheric and nest temperatures during reproduction in the zebra finch. We characterized the temperature within nests in a subtropical population of this species in relation to atmospheric temperature. Temperatures within nests frequently exceeded the level at which embryo's develop optimally, even in the absence of parental incubation. We experimentally manipulated internal nest temperature to demonstrate that an average difference of 6°C in the nest temperature during the laying period reduced hatching time by an average of 3% of the total incubation time, owing to 'ambient incubation'. Given the avian constraint of laying a single egg per day, the first eggs of a clutch are subject to prolonged effects of nest temperature relative to later laid eggs, potentially increasing hatching asynchrony. While birds may ameliorate the negative effects of ambient incubation on embryonic development by varying the location and design of their nests, high atmospheric temperatures are likely to constitute an important selective force on avian reproductive behaviour and physiology in subtropical and tropical regions, particularly in the light of predicted climate change that in many areas is leading to a higher frequency of hot days during the periods when birds breed.

Do cell-autonomous and non-cell-autonomous effects drive the structure of tumor ecosystems?

By definition, a driver mutation confers a growth advantage to the cancer cell in which it occurs, while a passenger mutation does not: the former is usually considered as the engine of cancer progression, while the latter is not. Actually, the effects of a given mutation depend on the genetic background of the cell in which it appears, thus can differ in the subclones that form a tumor. In addition to cell-autonomous effects generated by the mutations, non-cell-autonomous effects shape the phenotype of a cancer cell. Here, we review the evidence that a network of biological interactions between subclones drives cancer cell adaptation and amplifies intra-tumor heterogeneity. Integrating the role of mutations in tumor ecosystems generates innovative strategies targeting the tumor ecosystem's weaknesses to improve cancer treatment.

Outcropping reef ledges drive patterns of epibenthic assemblage diversity on cross-shelf habitats

Seafloor habitats on continental shelf margins are increasingly being the subject of worldwide conservation efforts to protect them from human activities due to their biological and economic value. Quantitative data on the epibenthic taxa which contributes to the biodiversity value of these continental shelf margins is vital for the effectiveness of these efforts, especially at the spatial resolution required to effectively manage theseecosystems. We quantified the diversity of morphotype classes on an outcropping reef system characteristic of the continental shelf margin in the Flinders Commonwealth Marine Reserve, southeastern Australia. The system is uniquely characterized by long linear outcropping ledge features in sedimentary bedrock that differ markedly from the surrounding low-profile, sand-inundated reefs. We characterize a reef system harboring rich morphotype classes, with a total of 55 morphotype classes identified from the still images captured by an autonomous underwater vehicle. The morphotype class Cnidaria/Bryzoa/Hydroid matrix dominated the assemblages recorded. Both a and b diversitydeclined sharply with distance from nearest outcropping reef ledge feature. Patterns of the morphotype classes were characterized by (1) morphotype turnover at scales of 5 to 10s m from nearest outcropping reef ledge feature, (2) 30 % of morphotype classes were recordedonly once (i.e. singletons), and (3) generally low levels of abundance (proportion cover) of the component morphotype class. This suggests that the assemblages in this region contain a considerable number of locally rare morphotype classes. This study highlights the particular importance of outcropping reef ledge features in this region, as they provide a refuge against sediment scouring and inundation common on the low profile reef that characterizes this region. As outcropping reef features, they represent a small fraction of overall reef habitat yet contain much of the epibenthic faunal diversity. This study has relevance to conservation planning for continental shelf habitats, as protecting a single, or few, areas of reef is unlikely to accurately represent the geomorphic diversity of cross-shelf habitats and the morphotype diversity that is associated with these features. Equally, whendesigning monitoring programs these spatially-discrete, but biologically rich outcropping reef ledge features should be considered as distinct components in stratified sampling designs.