Morphometric and molecular characterization of the species of Uncinaria Frölich, 1789 (Nematoda) parasitic in the Australian fur seal Arctocephalus pusillus doriferus (Schreber), with notes on hookworms in three other pinniped hosts

This study presents morphological and molecular data on hookworms from the Australian fur seal Arctocephalus pusillus doriferus (Schreber) currently identified in Australian waters as Uncinaria hamiltoni Baylis, 1933. Additional specimens from the Australian sea lion Neophoca cinerea (Péron) and the New Zealand fur seal Arctocephalus forsteri (Lesson) from Australia, and the Southern elephant seal Mirounga leonina (Linnaeus) from Antarctica, were included. Using the internal transcribed spacer (ITS), hookworms from A. p. doriferus, N. cinerea and A. forsteri were found to be genetically similar but distinct from Uncinaria spp. found in M. leonina from Antarctica, as well as from Zalophus californianus (Lesson) and Callorhinus ursinus (Linnaeus) from California. Few morphological differences were detected between these taxa.

Foxp3 expression in macrophages associated with RENCA tumors in mice.

The transcription factor Foxp3 represents the most specific functional marker of CD4+ regulatory T cells (TRegs). However, previous reports have described Foxp3 expression in other cell types including some subsets of macrophages, although there are conflicting reports and Foxp3 expression in cells other than Treg is not well characterized. We performed detailed investigations into Foxp3 expression in macrophages in the normal tissue and tumor settings. We detected Foxp3 protein in macrophages infiltrating mouse renal cancer tumors injected subcutaneously or in the kidney. Expression was demonstrated using flow cytometry and Western blot with two individual monoclonal antibodies. Further analyses confirmed Foxp3 expression in macrophages by RT PCR, and studies using ribonucleic acid-sequencing (RNAseq) demonstrated a previously unknown Foxp3 messenger (m)RNA transcript in tumor-associated macrophages. In addition, depletion of Foxp3+ cells using diphtheria toxin in Foxp3DTR mice reduced the frequency of type-2 macrophages (M2) in kidney tumors. Collectively, these results indicate that tumor-associated macrophages could express Foxp3.

"I'm in their shoes": Experiences of peer educators in sexuality and relationship education

Background: Sexuality and relationship education for adults with an intellectual disability has failed to include them in roles other than as learners. This paper reports findings from a study of the experiences of peer educators with an intellectual disability who co-facilitated a respectful relationships education program. Method: Qualitative data were collected about the experiences of 16 peer educators through in-depth interviews and observations of their work in delivering the program. These data were thematically analysed. Findings: Peer educators reported that peer education gave them a sense of empowerment, positioned them as credible sources of information about relationships, enabled them to help others, and gave them an opportunity to learn new knowledge about respectful relationships, community resources and supports, and new skills. Conclusions: This study presents an alternative approach to relationship education that involves people with an intellectual disability as peer educators and that benefits these people. © 2014 © 2014 Australasian Society for Intellectual Disability, Inc.

Cultural pedagogies in the museum: walking, listening and feeling

Pedagogy is often glossed as the ‘art and science of teaching’ but this focus typically ties it to the instructional practices of formalised schooling. Like the emerging work on ‘public pedagogies’, the notion of cultural pedagogies signals the importance of the pedagogic in realms other than institutionalised education, but goes beyond the notion of public pedagogies in two ways: it includes spaces which are not so public, and it includes an emphasis on material and non-human actors. This collection foregrounds this broader understanding of pedagogy by framing enquiry through a series of questions and across a range of settings. How, for example, are the processes of ‘teaching’ and ‘learning’ realised within and across the pedagogic processes specific to various social sites? What ensembles of people, things and practices are brought together in specific institutional and everyday settings to accomplish these processes? This collection brings together researchers whose work across the interdisciplinary nexus of cultural studies, sociology, media studies, education and museology offers significant insights into these ‘cultural pedagogies’ – the practices and relations through which cumulative changes in how we act, feel and think occur. Cultural Pedagogies and Human Conduct opens up debate across disciplines, theoretical perspectives and empirical foci to explore both what is pedagogical about culture and what is cultural about pedagogy.

Evaluation of the Test-mate ChE (cholinesterase) field kit in acute organophosphorus poisoning

STUDY OBJECTIVE: Measurement of acetylcholinesterase (AChE) is recommended in the management of organophosphorus poisoning, which results in 200,000 deaths worldwide annually. The Test-mate ChE 400 is a portable field kit designed for detecting occupational organophosphorus exposure that measures RBC AChE and plasma cholinesterase (PChE) within 4 minutes. We evaluate Test-mate against a reference laboratory test in patients with acute organophosphorus self-poisoning. METHODS: This was a cross-sectional comparison study of 14 patients with acute organophosphorus poisoning between May 2007 and June 2008. RBC AChE and PChE were measured in 96 and 91 samples, respectively, with the Test-mate ChE field kit and compared with a reference laboratory, using the limits of agreement method (Bland and Altman), κ statistics, and Spearman's correlation coefficients. RESULTS: There was good agreement between the Test-mate ChE and the reference laboratory for RBC AChE. The mean difference (Test-mate-reference) was -0.62 U/g hemoglobin, 95% limits of agreement -10.84 to 9.59 U/g hemoglobin. Good agreement was also observed between the categories of mild, moderate, and severe RBC AChE inhibition (weighted κ 0.85; 95% confidence interval [CI] 0.83 to 0.87). Measurement of PChE also showed good agreement, with a mean difference (Test-mate-reference) of +0.06 U/mL blood, 95% limits of agreement -0.41 to 0.53 U/mL blood. Spearman's correlation coefficients were 0.87 (95% CI 0.81 to 0.91) for RBC AChE and 0.76 (95% CI 0.66 to 0.84) for PChE. Analysis for within-subject correlation of subjects did not change the limits of agreement. CONCLUSION: The Test-mate ChE field kit reliably provides rapid measurement of RBC AChE in acute organophosphorus poisoning.

A novel route for tethering graphene with iron oxide and its magnetic field alignment in polymer nanocomposites

We present a new route of tethering graphene nanoplatelets (GNPs) with Fe3O4 nanoparticles to enable their alignment in an epoxy using a weak magnetic field. The GNPs are first stabilised in water using polyvinylpyrrolidone (PVP) and Fe3O4 nanoparticles are then attached via co-precipitation. The resultant Fe3O4/PVP-GNPs nanohybrids are superparamagnetic and can be aligned in an epoxy resin, before gelation, by applying a weak magnetic field as low as 0.009 T. A theoretical model describing the alignment process is presented and used to quantify the effects of key parameters on the time needed for the alignment process. Compared to the unmodified epoxy, the resulting epoxy polymer nanocomposites containing randomly-oriented Fe3O4/PVP-GNPs nanohybrids exhibit significantly improved electrical conductivities by up to three orders of magnitude and fracture energies by up to 300%. The alignment of the Fe3O4/PVP-GNPs nanohybrids in the epoxy polymer nanocomposites transverse to the direction of crack propagation further increased the fracture energy by 50%, and the electrical conductivity by seven fold in the alignment direction, compared to the nanocomposites containing randomly-oriented nanohybrids.

Deformation field variations in equal channel angular extrusion due to back pressure

Flow lines were analysed in aluminium alloy 6061 during equal channel angular extrusion (ECAE) in a 90° die with and without the application of back pressure during pressing. The lines appeared much more rounded when a back pressure was applied compared to the case of conventional ECAE testing. With the help of an analytic flow function, the deformation field was obtained. It is shown that back pressure slightly lowers the total strain, strongly increases the size of the plastic zone and significantly reduces the plastic strain rate.

Correlation of throwing velocity to the results of lower-body field tests in male college baseball players

Baseball-specific athleticism, potential, and performance have been difficult to predict. Increased muscle strength and power can increase throwing velocity but the majority of research has focused on the upper body. The present study sought to determine if bilateral or unilateral lower-body field testing correlates with throwing velocity. Baseball throwing velocity scores were correlated to the following tests: medicine ball (MB) scoop toss and squat throw, bilateral and unilateral vertical jumps, single and triple broad jumps, hop and stop in both directions, lateral to medial jumps, 10- and 60-yd sprints, and both left and right single-leg 10-yd hop for speed in 42 college baseball players. A multiple regression analysis (forward method), assessing the relationship between shuffle and stretch throwing velocities and lower-body field test results determined that right-handed throwing velocity from the stretch position was most strongly predicted by lateral to medial jump right (LMJR) and body weight (BW; R = 0.322), whereas lateral to medial jump left (LMJL; R = 0.688) predicted left stretch throw. Right-handed shuffle throw was most strongly predicted by LMJR and MB scoop (R = 0.338), whereas LMJL, BW, and LMJR all contributed to left-handed shuffle throw (R = 0.982). Overall, this study found that lateral to medial jumps were consistently correlated with high throwing velocity in each of the throwing techniques, in both left-handed and right-handed throwers. This is the first study to correlate throwing velocity with a unilateral jump in the frontal plane, mimicking the action of the throwing stride.

The applicability of self-regulation theories in sport: Goal adjustment capacities, stress appraisals, coping, and well-being among athletes

We examined a model, informed by self-regulation theories from the health psychology literature, which included goal adjustment capacities, appraisals of challenge and threat, coping, and well-being. Two-hundred and twelve athletes from the United Kingdom (n 147)= or Australia (n = 65), who played team (n = 135) or individual sports (n = 77), and competed at international (n = 7), national (n = 11), county (n = 67), club (n = 84), or beginner (n = 43) levels participated in this study. Participants completed measures of goal adjustment capacities and stress appraisals two days before competing. Athletes also completed coping and well-being questionnaires within three hours of their competition ending.The way an athlete responded to an unattainable goal was associated with his or her well-being in the period leading up to and including the competition. Goal reengagement positively predicted well-being, whereas goal disengagement negatively predicted well-being. Further, goal reengagement was positively associated with challenge appraisals, which in turn was linked to task-oriented coping, and task-oriented coping positively associated with well-being.When highly-valued goals become unattainable, consultants and coaches could encourage athletes to generate alternative approaches to achieve the same goal or help them develop a completely new goal in order to promote well-being among athletes.