Reduced glycogen availability is associated with increased AMPKα2 activity, nuclear AMPKα2 protein abundance, and GLUT4 mRNA expression in contracting human skeletal muscle

Glycogen availability can influence glucose transporter 4 (GLUT4) expression in skeletal muscle through unknown mechanisms. The multisubstrate enzyme AMP-activated protein kinase (AMPK) has also been shown to play an important role in the regulation of GLUT4 expression in skeletal muscle. During contraction, AMPK [alpha]2 translocates to the nucleus and the activity of this AMPK isoform is enhanced when skeletal muscle glycogen is low. In this study, we investigated if decreased pre-exercise muscle glycogen levels and increased AMPK [alpha]2 activity reduced the association of AMPK with glycogen and increased AMPK [alpha]2 translocation to the nucleus and GLUT4 mRNA expression following exercise. Seven males performed 60 min of exercise at ~70% [VO.sub.2] peak on 2 occasions: either with normal (control) or low (LG) carbohydrate pre-exercise muscle glycogen content. Muscle samples were obtained by needle biopsy before and after exercise. Low muscle glycogen was associated with elevated AMPK [alpha]2 activity and acetyl-CoA carboxylase [beta] phosphorylation, increased translocation of AMPK [alpha]2 to the nucleus, and increased GLUT4 mRNA. Transfection of primary human myotubes with a constitutively active AMPK adenovirus also stimulated GLUT4 mRNA, providing direct evidence of a role of AMPK in regulating GLUT4 expression. We suggest that increased activation of AMPK [alpha]2 under conditions of low muscle glycogen enhances AMPK [alpha]2 nuclear translocation and increases GLUT4 mRNA expression in response to exercise in human skeletal muscle.

Attitudes and knowledge of shade-coffee farmers towards vertebrates and their ecological functions.

The purpose of this study was to assess farmers’ attitudes, as well as perceptions and knowledge that shape those attitudes, toward the ecological role of vertebrates inhabiting shaded-coffee farms. We also aimed to determine whether differences existed among two groups of farmers: one that had attended environmental education workshops, and one that had not. We conducted 36 oral interviews of farmers in the region of Cuetzalan, Mexico. All farmers were members of an important regional cooperative, Tosepan Titataniske. In general, farmers’ attitudes towards birds were positive. Snakes were perceived as useful but dangerous animals. Attitudes towards nonflying mammals were mostly indifferent. Bats were poorly understood and badly perceived. Seed dispersal was perceived as an important ecological function performed by animals. Pollination was also perceived as important, but to a lesser degree. Knowledge about ecological functions was high for seed dispersal, and low for pollination. We found a positive correlation between attendance of educational workshops and the presence of “environmentally-friendly” attitudes, perceptions, and knowledge. However, a cause-effect relationship could not be clearly established. We suggest that environmental education programs include the objective of increasing the knowledge of people about the ecological functions played by different groups of animals that live in agroecosystems. Particular efforts should be directed toward improving the way in which certain non-charismatic groups of animals, such as bats, are perceived.

Characterisation and physiological functions of plant natriuretic peptides

Natriuretic peptides are bioactive proteins. In plants, biochemical and physiological studies on these molecules has now revealed that they influence stomatal opening, cell volume and the activity of membrane pumps and their localisation within vascular tissues. Thus they have major roles in maintaining water and solute homeostasis.

Contracting-agency analysis of the association between firm risk, investment opportunity set, corporate governance and firm performance

Tests the efficiency of corporate controls (board monitoring and incentive contracts) for growth or risk firms. By exploring the implications of controls and studying their interactive effects on firm performance, this study demonstrates how and why different firms use corporate governance controls to align managers' and shareholders' interests.

Construction of aggregation functions from data using linear programming

This article examines the construction of aggregation functions from data by minimizing the least absolute deviation criterion. We formulate various instances of such problems as linear programming problems. We consider the cases in which the data are provided as intervals, and the outputs ordering needs to be preserved, and show that linear programming formulation is valid for such cases. This feature is very valuable in practice, since the standard simplex method can be used.

Aggregation functions based on penalties

This article studies a large class of averaging aggregation functions based on minimizing a distance from the vector of inputs, or equivalently, minimizing a penalty imposed for deviations of individual inputs from the aggregated value. We provide a systematization of various types of penalty based aggregation functions, and show how many special cases arise as the result. We show how new aggregation functions can be constructed either analytically or numerically and provide many examples. We establish connection with the maximum likelihood principle, and present tools for averaging experimental noisy data with distinct noise distributions.

On Lipschitz properties of generated aggregation functions

This article discusses Lipschitz properties of generated aggregation functions. Such generated functions include triangular norms and conorms, quasi-arithmetic means, uninorms, nullnorms and continuous generated functions with a neutral element. The Lipschitz property guarantees stability of aggregation operations with respect to input inaccuracies, and is important for applications. We provide verifiable sufficient conditions to determine when a generated aggregation function holds the k-Lipschitz property, and calculate the Lipschitz constants of power means. We also establish sufficient conditions which guarantee that a generated aggregation function is not Lipschitz. We found the only 1-Lipschitz generated function with a neutral element e ∈]0, 1[.

The use of prolog in the realisation of digital transfer functions

Systems characterised by general graphs are very flexible because of a given application they offer a great number of degrees of freedom and consequently a great possibility of choice to the designer. This can become a drawback in practice when myriads of cases have to be examined analytically and most of them rejected for a variety of heterogeneous reasons. For such design problems, it appears that logic programming languages are ideally suited because they can implement the heterogeneous rules which describe the desired system in a natural way. An application of the Prolog language to the design of active RC biquad circuits investigated by Mouly in his doctoral thesis illustrates the advantages of such a rule-based approach.

Identification of nonlinear systems using hybrid functions

Most real systems have nonlinear behavior and thus model linearization may not produce an accurate representation of them. This paper presents a method based on hybrid functions to identify the parameters of nonlinear real systems. A hybrid function is a combination of two groups of orthogonal functions: piecewise orthogonal functions (e.g. Block-Pulse) and continuous orthogonal functions (e.g. Legendre polynomials). These functions are completed with an operational matrix of integration and a product matrix. Therefore, it is possible to convert nonlinear differential and integration equations into algebraic equations. After mathematical manipulation, the unknown linear and nonlinear parameters are identified. As an example, a mechanical system with single degree of freedom is simulated using the proposed method and the results are compared against those of an existing approach.

Dipole time correlation functions of the Stockmayer fluid in the microcanonical and canonical ensembles

Computer simulations of Stockmayer fluids were performed to generate dipole time correlation functions (TCF) at three temperatures and three dipole moments in both the microcanonical and canonical ensembles. The effect of Nosé constant-temperature dynamics on time-dependent quantities is discussed, and empirical results are given to show that the choice of thermal inertia parameter influences the speed with which a system moves through its phase space. The time correlation functions from the simulations were analyzed in terms of current theories for dipolar systems. A functional form is proposed to cover both the longtime and short-time behavior of the time correlation functions of dipoles. The relationship between this functional form and the dielectric function of the Stockmayer system is also discussed.

On a class of contest success functions

In this article, I analyze a class of contest success functions (CSFs) that satisfy Luce's Choice Axiom. I show that the functional forms of these CSFs can be fully identified if they are characterized by a partial differential equation (PDE), which has several intuitive economic interpretations. This POE approach provides foundations for popular CSFs and their generalizations.

Optimization and aggregation functions

In this work we will look at connections between aggregation functions and optimization. There are two such connections: 1) aggregation functions are used to transform a multiobjective optimization problem into a single objective problem by aggregating several criteria into one, and 2) construction of aggregation functions often involves an optimization problem.