Where and when does a ring start and end? Testing the ring-species hypothesis in a species complex of Australian parrots

Speciation, despite ongoing gene flow can be studied directly in nature in ring species that comprise two reproductively isolated populations connected by a chain or ring of intergrading populations. We applied three tiers of spatio-temporal analysis (phylogeny/historical biogeography, phylogeography and landscape/population genetics) to the data from mitochondrial and nuclear genomes of eastern Australian parrots of the Crimson Rosella Platycercus elegans complex to understand the history and present genetic structure of the ring they have long been considered to form. A ring speciation hypothesis does not explain the patterns we have observed in our data (e.g. multiple genetic discontinuities, discordance in genotypic and phenotypic assignments where terminal differentiates meet). However, we cannot reject that a continuous circular distribution has been involved in the group's history or indeed that one was formed through secondary contact at the 'ring's' east and west; however, we reject a simple ring-species hypothesis as traditionally applied, with secondary contact only at its east. We discuss alternative models involving historical allopatry of populations. We suggest that population expansion shown by population genetics parameters in one of these isolates was accompanied by geographical range expansion, secondary contact and hybridization on the eastern and western sides of the ring. Pleistocene landscape and sea-level and habitat changes then established the birds' current distributions and range disjunctions. Populations now show idiosyncratic patterns of selection and drift. We suggest that selection and drift now drive evolution in different populations within what has been considered the ring.

Does the ring species concept predict vocal variation in the crimson rosella, Platycercus elegans, complex?

Vocal variation may be important in population divergence. We studied geographical variation in contact calls of parrots of the crimson rosella, Platycercus elegans, complex, which is characterized by striking geographical plumage coloration variation. This complex has long been considered a rare example of a ring species (where two divergent forms coexist in sympatry but are connected by a chain of intermediate populations forming a geographical ring). We tested whether contact call variation is consistent with the ring species hypothesis. We recorded calls throughout the ring, including several sites from the three main population groups forming the ring and interfaces between them. We analysed duration, peak frequency, fundamental frequency and frequency modulation. We found significant differences, particularly in fundamental frequency and frequency modulation, at multiple biogeographical scales ranging from local populations to subspecies level. Discriminant function analyses showed some populations could be reliably discriminated from call structure. However, our results provided little support for three key predictions of the ring species hypothesis: (1) calls of the terminal, most divergent forms were not significantly different in three of the four acoustic variables, and differences did not appear to be maintained in sympatry, (2) phenotypically/geographically intermediate populations were not characterized by intermediate calls, and (3) call variation was not concordant with geographical sequence around the ring from one terminal form to the other. Our results underscore the emerging view that the evolutionary histories and phenotypic variability of many long-held ring species may be inadequately described by the ring species hypothesis and require alternative explanations. (C) 2008 The Association for the Study of Animal Behaviour. Published by Elsevier Ltd. All rights reserved.

Coloration and colour vision in a parrot ring species

Ring species, in which reproductively isolated forms are connected by a chain of intermediate populations, provide valuable insights into the maintenance of trait variability, divergence in sympatry, and the how small changes can lead to species level differences. The parrot Platycercus elegans of eastern Australia is highly variable in plumage coloration in the wild, ranging from pale yellow to deep crimson in the chest, rump and head. It has been suggested as the only known parrot ring species worldwide, and one of less than ~25 ring species amongst all organisms. We test hypotheses for the information signalled by the colour variation, and for the maintenance of the variability.

Multi-level virtual ring : a foundation network architecture to support peer-to-peer application in wireless sensor network

Two main problems prevent the deployment of peer-to-peer application in a wireless sensor network: the index table, which should be distributed stored rather than uses a central server as the director; the unique node identifier, which cannot use the global addresses. This paper presents a multi-level virtual ring (MVR) structure to solve these two problems.

The index table in MVR is distributed stored by using the DHT technique. MVR is constructed decentralized and runs on mobile nodes themselves, requiring no central server or interruption. Naming system in MVR uses natural names rather than global addresses to identify sensor nodes. The MVR can route directly on the name identifiers of the sensor nodes without being aware the location. Some sensor nodes are selected as the backbone nodes by the backbone selection algorithm and are placed on the different levels of the virtual rings. MVR hashes nodes’ identifiers on the virtual ring, and stores them at the backbone nodes. Furthermore, MVR adopts cross-level routing to improve the routing efficiency.

Experiments using ns2 simulator for up to 200 nodes show that the storage and bandwidth requirements of MVR grow slowly with the size of the network. Furthermore, MVR has demonstrated as self-administrating, fault-tolerant, and resilient under the different workloads.

Multi-level virtual ring : cross-level name routing with embedded identifier in sensor network

This paper presents the Multi-level Virtual Ring (MVR), a new name routing scheme for sensor networks. MVR uses selection algorithm to identify sensor nodes' virtual level and uses Distribution Hash Table (DHT) to map them to the MVR, The address routing performs well in wired network, but it's not true in sensor network. Because when nodes are moving, the address of the nodes must be changed Further, the address routing needs servers to allocate addresses to nodes. To solve this problem, the name routing is being introduced, such as Virtual Ring Routing (VRR). MVR is a new name routing scheme, which improves the routing performance significantly by introducing the multi-level virtual ring and cross-level routing. Experiments show this embedded name routing is workable and achieves better routing performance.

Boroxine ring compounds as dissociation enhancers in gel polyelectrolytes

In order to combine the advantages of both traditional gel electrolytes and polyelectrolytes, a novel polyelectrolyte which incorporates a boroxine ring-containing anion-trapping agent has been explored. Poly(lithium 2-acrylamido-2-methyl-1-propanesulfonate) (PAMPSLi), ethylene carbonate (EC) and tri(methoxyethoxyethoxyethoxy)boroxine (TME₃Bx) were combined to prepare various gel systems. The thermal properties and conductivities of these gels have been investigated. A conductivity of 10^−3.6 S cm⁻¹ at 20 °C has been achieved in a gel polyelectrolyte system with a molar ratio of [EC]:[TME3Bx]:[Li⁺]=24:1.7:1. Temperature-dependent NMR measurements indicated that a significant interaction exists between the boroxine ring and the polyelectrolyte.

Improving wool/polyester yarn quality by transposal spinning with movable balloon control ring

To improve yarn quality in ring spinning frame, this paper examines transposal spinning with the movable balloon control ring. The left-transposal spinning and the right-transposal spinning were experimented to spin two yarns; after installing the movable balloon control ring, the two methods were respectively used to spin two other yarns again. All the experiments were on the same ring frame and the raw material was wool/polyester blend roving with a ration of 30/70. The main quality indexes of the four yarns were tested and analyzed, including hairiness, tensile property, evenness and usual faults. The results show that the yarn spun by the left-transposal spinning has a better quality than the right-transposal spinning, and after adding the movable balloon control ring, the improvement of yarn hairiness strength and evenness, as well usual faults, is very obvious. So, in the advantage of transposal spinning, the method of installing the movable balloon control ring can improve some deteriorative problems and make yarn quality better. In addition, the method gives new insight into energy efficiency. The research in this field in on the way and the result is in accord with the Low-carbon Economy.

Innervation zone shift at different levels of isometric contraction in the biceps brachii muscle

Experiments were carried out to examine whether innervation zone (IZ) location remains stable at different levels of isometric contraction in the biceps brachii muscle (BB), and to determine how the proximity of the IZ affects common surface electromyography (sEMG) parameters. Twelve subjects performed maximal (MVC) and submaximal voluntary isometric contractions at 10%, 20%, 30%, 40%, 50% and 75% of MVC. sEMG signals were recorded with a 13 rows  5 columns grid of electrodes from the short head of BB. The IZ shifted in the proximal direction by up to 2.4 cm, depending upon the subject and electrode column. The mean shift of all the columns was 0.6 ± 0.4 cm (10% vs. 100% MVC, P < 0.001). This shift biased the average values of mean frequency (+21.8 ± 9.9 Hz, P < 0.001), root mean square (0.16 ± 0.15 mV, P < 0.05) and conduction velocity (1.15 ± 0.93 m/s, P < 0.01) in the channels immediately proximal to the IZ. The shift in IZ could be explained by shortening of the muscle fibers, and thus lengthening of the (distal) tendon due to increasing force. These results underline the importance of individual investigation of IZ locations before the placement of sEMG electrodes, even in isometric contractions.