Control of excitation and quenching in multi-colour electrogenerated chemiluminescence systems through choice of co-reactant

We demonstrate a new approach to manipulate the selective emission in mixed electrogenerated chemiluminescence (ECL) systems, where subtle changes in co-reactant properties are exploited to control the relative electron-transfer processes of excitation and quenching. Two closely related tertiary-amine co-reactants, tri-n-propylamine and N,N-diisopropylethylamine, generate remarkably different emission profiles: one provides distinct green and red ECL from [Ir(ppy)3] (ppy=2-phenylpyridinato-C2,N) and a [Ru(bpy)3](2+) (bpy=2,2'-bipyridine) derivative at different applied potentials, whereas the other generates both emissions simultaneously across a wide potential range. These phenomena can be rationalized through the relative exergonicities of electron-transfer quenching of the excited states, in conjunction with the change in concentration of the quenchers over the applied potential range.

A parametric study of a monolithic microfluidic system for on-chip biomolecular separation

A microfabricated poly(dimethylsiloxane) (PDMS) chip containing channel filled with polymer monolith has been developed for on-chip biomolecule separation. Methacrylate monolithic polymers were prepared by photo-initiated polymerization within the channel to serve as a continuous stationary phase. The monolithic polymer was functionalized with a weak anion-exchange ligand, and key parameters affecting the binding characteristics of the system were investigated. The total binding capacity was unaffected by the flow rate of the mobile phase but varied significantly with changes in ionic strength and pH of the binding buffer. The binding capacity decreased with increasing buffer ionic strength, and this is due to the limited available binding sites for protein adsorption resulting from cationic shielding effect. Similarly, the binding capacity decreased with decreasing buffer pH towards the isoelectric point of the protein. A protein mixture, BSA and ovalbumin, was used to illustrate the capacity of the methacrylate-based microfluidic chip for rapid biomolecule separation.

Parametric investigation of batch adsorption of proteins onto polymeric particles

Background: Effective bimolecular adsorption of proteins onto solid matrices is characterized by in-depth understanding of the biophysical features essential to optimize the adsorption performance. Results: The adsorption of bovine serum albumin (BSA) onto anion-exchange Q-sepharose solid particulate support was investigated in batch adsorption experiments. Adsorption kinetics and isotherms were developed as a function of key industrially relevant parameters such as polymer loading, stirring speed, buffer pH, protein concentration and the state of protein dispersion (solid/aqueous) in order to optimize binding performance and adsorption capacity. Experimental results showed that the first order rate constant is higher at higher stirring speed, higher polymer loading, and under alkaline conditions, with a corresponding increase in equilibrium adsorption capacity. Increasing the stirring speed and using aqueous dispersion protein system increased the adsorption rate, but the maximum protein adsorption was unaffected. Regardless of the stirring speed, the adsorption capacity of the polymer was 2.8 mg/ml. However, doubling the polymer loading increased the adsorption capacity to 9.4 mg/ml. Conclusions: The result demonstrates that there exists a minimum amount of polymer loading required to achieve maximum protein adsorption capacity under specific process conditions.

A nonlinear adaptive backstepping approach for coordinated excitation and steam-valving control of synchronous generators

Steam-valving and excitation systems play an important role to maintain the transient stability of power systems with synchronous generators when power systems are subjected to large disturbances and sudden load changes. This paper presents a nonlinear adaptive backstepping approach for controlling excitation and steam-valving systems of synchronous generators. In this paper, the proposed excitation and steam-valving controllers are designed in a coordinated manner so that they can work under several and most severe operating conditions. Both excitation and steam-valving controllers are designed by considering some critical parameters as unknown. The effectiveness of the proposed coordinated control scheme is evaluated on a single machine infinite bus system under different operating conditions such as load changes and three-phase short circuit faults at the generator terminal. Finally, performance of the proposed scheme is compared to that of a similar nonlinear adaptive backstepping excitation controller without any coordination and simulation results demonstrate the superiority of the proposed one.

Robust adaptive backstepping excitation controller design for simple power system models with external disturbances

This paper presents a nonlinear robust adaptive excitation controller design for a simple power system model where a synchronous generator is connected to an infinite bus. The proposed controller is designed to obtain the adaption laws for estimating critical parameters of synchronous generators which are considered as unknown while providing the robustness against the bounded external disturbances. The convergence of different physical quantities of a single machine infinite bus (SMIB) system, with the proposed control scheme, is ensured through the negative definiteness of the derivative of Lyapunov functions. The effects of external disturbances are considered during formulation of Lyapunov function and thus, the proposed excitation controller can ensure the stability of the SMIB system under the variation of critical parameters as well as external disturbances including noises. Finally, the performance of the proposed scheme is investigated with the inclusion of external disturbances in the SMIB system and its superiority is demonstrated through the comparison with an existing robust adaptive excitation controller. Simulation results show that the proposed scheme provides faster responses of physical quantities than the existing controller.

Nonlinear excitation control of synchronous generators based on adaptive backstepping method

n this paper, the design of a nonlinear excitation control of a synchronous generator is presented where the generator is connected to a single machine infinite bus (SMIB) system. An adaptive backstepping method is used to design the excitation controller with an objective of enhancing the overall dynamic stability of the SMIB system under different contingencies. In this paper, two types of contingencies are considered- i) unknown parameters and physical quantities during the controller design process and ii) controller performance evaluation under different system configurations such as three-phase short circuit faults. The adaption law, which is mainly based on the formulation of Lyapunov function, is used to estimate the unknown parameters which guarantee the convergence of different physical quantities of synchronous generators, e.g., the relative speed, terminal voltage, etc. The effectiveness of the proposed scheme is evaluated under different system configurations as mentioned in the second contingency and compared to that of an existing adaptive backstepping controller and a conventional power system stabilizer (PSS). Simulation results demonstrate the superiority of the proposed control scheme over the existing controllers.

Nonlinear adaptive excitation controller design for multimachine power systems

This paper presents a nonlinear adaptive excitation control scheme to enhance the dynamic stability of multimachine power systems. The proposed controller is designed based on the adaptive backstepping technique where the mechanical power input to the generators and the damping coefficient of each generator are considered as unknown. These unknown quantities are estimated through the adaption laws. The adaption laws are obtained from the formulation of Lyapunov functions which guarantee the convergence of different physical quantities of generators such as the relative speed, terminal voltage, and electrical power output. The proposed scheme is evaluated by applying a three-phase short-circuit fault at one of the key transmission lines in an 11-bus test power system and compared with an existing backstepping controller and conventional power system stabilizer (CPSS). Simulation results show that the proposed scheme is much more effective than existing controllers.