RNA interference : more than a research tool in the vertebrates' adaptive immunity

In recent years, RNA silencing, usage of small double stranded RNAs of ~21 – 25 base pairs to regulate gene expression, has emerged as a powerful research tool to dissect the role of unknown host cell factors in this 'post-genomic' era. While the molecular mechanism of RNA silencing has not been precisely defined, the revelation that small RNA molecules are equipped with this regulatory function has transformed our thinking on the role of RNA in many facets of biology, illustrating the complexity and the dynamic interplay of cellular regulation. As plants and invertebrates lack the protein-based adaptive immunity that are found in jawed vertebrates, the ability of RNA silencing to shut down gene expression in a sequence-specific manner offers an explanation of how these organisms counteract pathogen invasions into host cells. It has been proposed that this type of RNA-mediated defence mechanism is an ancient form of immunity to offset the transgene-, transposon- and virus-mediated attack. However, whether 1) RNA silencing is a natural immune response in vertebrates to suppress pathogen invasion; or 2) vertebrate cells have evolved to counteract invasion in a 'RNA silencing' independent manner remains to be determined. A number of recent reports have provided tantalizing clues to support the view that RNA silencing functions as a physiological response to regulate viral infection in vertebrate cells. Amongst these, two manuscripts that are published in recent issues of Science and Immunity, respectively, have provided some of the first direct evidences that RNA silencing is an important component of antiviral defence in vertebrate cells. In addition to demonstrating RNA silencing to be critical to vertebrate innate immunity, these studies also highlight the potential of utilising virus-infection systems as models to refine our understanding on the molecular determinants of RNA silencing in vertebrate cells.

Investment performance within urban regeneration locations

Purpose – Property performance indices have invariably focused upon prime markets with a variety of approaches used to measure investment returns. However, there is relatively little knowledge regarding the investment performance of property in regeneration areas. Indeed, there is a perception that such locations carry increased risk and that the returns achieved may not be sufficient to offset the added risk. The main objective of this paper, therefore, is to construct regeneration property performance indicators consistent with the CBRE rent index and average yield monitor.

Design/methodology/approach – Local market experts were asked to estimate rents and yields for hypothetical standardised offerings for a range of regeneration locations throughout the UK, covering the period 1995 to 2002.

Findings – The results show that rental growth was similar in regeneration locations compared to the prime market. However, the analysis highlights a major yield shift for property in regeneration areas in the short to medium term. The downward pressure in yields would suggest that once a regeneration area becomes established and rental growth emerges, investor interest is stimulated resulting in increased competition and a shortening of yields.

Originality/value – The significance of this research is the quantification of property investment performance from regeneration areas that previously has not been available to investment institutions and decision makers. From a policy perspective this analysis is of relevance in confirming the maturing of locations that have received high levels of public sector support and indicating the effectiveness of regeneration policy mechanisms in creating sustainable urban environments capable of meeting private sector investment goals.

An empirical analysis of the effects of the exchange rate on Canadian tourism

The effects of depreciation of the Canadian dollar on the Canadian tourist industry are estimated, and it is shown that the exchange rate had a modest impact in attracting U. S. visitors to Canada. However, the favorable exchange rate effects seem to be offset by other factors.

Do financial reforms improve the performance of financial holding companies? The case of Taiwan

We examine the performance of financial holding companies (FHCs) in Taiwan after the financial reform that removes the separation of banking, securities, insurance, and other financial services. Using data envelopment analysis, we find that FHCs fail to improve technical efficiencies in the post-reform era. They also do not outperform independent commercial banks after the financial reform. Lower technical efficiency caused by excess operating expenses appears to be the primary source of inefficiency. While scale efficiency may improve as FHCs grow larger, the benefits are marginal and insufficient to offset the potential costs of organizational diseconomies. Our findings suggest that increasing the size and scope of financial activities alone do not necessarily improve the performance of financial firms.

Maternal dietary intake during pregnancy has longstanding consequences for the health of her offspring

Over the past 100 years, advances in pharmaceutical and medical technology have reduced the burden of communicable disease, and our appreciation of the mechanisms underlying the development of noncommunicable disease has broadened. During this time, a number of studies, both in humans and animal models, have highlighted the importance of maintaining an optimal diet during pregnancy. In particular, a number of studies support the hypothesis that suboptimal maternal protein and fat intake during pregnancy can have long-term effects on the growing fetus, and increase the likelihood of these offspring developing cardiovascular, renal, or metabolic diseases in adulthood. More recently, it has been shown that dietary intake of a number of micronutrients may offset or reverse the deleterious effects of macronutrient imbalance. Furthermore, maternal fat intake has also been identified as a major contributor to a healthy fetal environment, with a beneficial role for unsaturated fats during development as well as a beneficial impact on cell membrane physiology. Together these studies indicate that attempts to optimise maternal nutrition may prove to be an efficient and cost-effective strategy for preventing the development of cardiovascular, renal, or metabolic diseases.

Workplace stressors for investigative interviewers of child-abuse victims

Purpose - This study aimed to identify the nature and prevalence of workplace stressors faced by interviewers of child sexual assault victims.

Design/methodology/approach - Sixty-eight professionals (police and child protection workers) were invited to anonymously post their perceptions of workplace stressors on an internet forum as part of an investigative interviewing online training course. Specifically, participants were asked to reflect on salient sources of stress encountered in their role of interviewing sexually abused children.

Findings - Three key stressors were identified across the study’s professional groups: (1) inadequate recognition of specialised skills; (2) high workload demands; and (3) interagency tensions. Consistent with previous research, exposure to child abuse reports was not raised as a stressor.

Research limitations/implications - Our study generated suggestions for modifying management practices; however, future research should identify and trial strategies for improving workplace climate in child abuse investigation.

Practical implications - As the stressors isolated by participants related to workplace climate rather than exposure to victims’ accounts of child abuse, minimising negative consequences of work stressors requires changes to workplace culture and practice. Workplace climates need to be modified so that the demands are offset by resources.

Originality/value - Because of its online, anonymous nature, this was the first study to offer participants the opportunity to honestly disclose primary sources of stress in child abuse investigation. The research also makes a much-needed contribution to an area of police practice that is vital yet often overlooked.

Does prey size matter? Novel observations of feeding in the leatherback turtle (Dermochelys coriacea) allow a test of predator–prey size relationships

Optimal foraging models predict that large predators should concentrate on large prey in order to maximize their net gain of energy intake. Here, we show that the largest species of sea turtle, Dermochelys coriacea, does not strictly adhere to this general pattern. Field observations combined with a theoretical model suggest that a 300 kg leatherback turtle would meet its energetic requirements by feeding for 3–4 h a day on 4 g jellyfish, but only if prey were aggregated in high-density patches. Therefore, prey abundance rather than prey size may, in some cases, be the overriding parameter for foraging leatherbacks. This is a classic example where the presence of small prey in the diet of a large marine predator may reflect profitable foraging decisions if the relatively low energy intake per small individual prey is offset by high encounter rates and minimal capture and handling costs. This study provides, to our knowledge, the first quantitative estimates of intake rate for this species.

The constructional geometry of early Javanese temples

The early development of Hindu Javanese architecture can be traced through interpretation of epigraphs, archaeological excavations, and comparison of extant temples with other traditions. However, while many scholars have speculated on connections between Javanese Hindu temples and presumed antecedents in India, these have been made on the basis of visual comparison and epigraphic interpretations. No Indian temple has been conclusively shown to be a model for the earliest Javanese temples. Archaeologist and temple historian Michael Meister has shown in his analysis of the geometric composition of early Hindu temples in South Asia how a ritual sixty-four square mandala was the geometric basis of temple construction during the formative period (fifth to eighth century) of the Indian architectural tradition. Working from an understanding of temple construction sequence as well as their ritual underpinnings, Meister found that the sixtyfour square mandala's dimensions correlate closely to the constructed dimensions at the level of the vedibandha (which corresponds with the plan level of the sanctuary threshold). Furthermore, he shows how the horizontal profile of the cella depends on the number of offsets and the proportional relationships between ech offset based on the subdivision of the sixty-four square grid. The authors have investigated whether a similar compositional basis can be found for the earliest Javanese temples on the Dieng Plateau in the highlands of central Java, despite differences in architectonic and symbolic expression. The analysis of relationships between ritual geometry and actual temple layouts for these buildings has the potential to furthering our understanding of the connections between Hindu temples in Java and those in India.

One year of sitagliptin treatment protects against islet amyloid-associated β-cell loss and does not induce pancreatitis or pancreatic neoplasia in mice

The dipeptidyl peptidase-4 (DPP-4) inhibitor sitagliptin is an attractive therapy for diabetes, as it increases insulin release and may preserve β-cell mass. However, sitagliptin also increases β-cell release of human islet amyloid polypeptide (hIAPP), the peptide component of islet amyloid, which is cosecreted with insulin. Thus, sitagliptin treatment may promote islet amyloid formation and its associated β-cell toxicity. Conversely, metformin treatment decreases islet amyloid formation by decreasing β-cell secretory demand and could therefore offset sitagliptin's potential proamyloidogenic effects. Sitagliptin treatment has also been reported to be detrimental to the exocrine pancreas. We investigated whether long-term sitagliptin treatment, alone or with metformin, increased islet amyloid deposition and β-cell toxicity and induced pancreatic ductal proliferation, pancreatitis, and/or pancreatic metaplasia/neoplasia. hIAPP transgenic and nontransgenic littermates were followed for 1 yr on no treatment, sitagliptin, metformin, or the combination. Islet amyloid deposition, β-cell mass, insulin release, and measures of exocrine pancreas pathology were determined. Relative to untreated mice, sitagliptin treatment did not increase amyloid deposition, despite increasing hIAPP release, and prevented amyloid-induced β-cell loss. Metformin treatment alone or with sitagliptin decreased islet amyloid deposition to a similar extent vs untreated mice. Ductal proliferation was not altered among treatment groups, and no evidence of pancreatitis, ductal metaplasia, or neoplasia were observed. Therefore, long-term sitagliptin treatment stimulates β-cell secretion without increasing amyloid formation and protects against amyloid-induced β-cell loss. This suggests a novel effect of sitagliptin to protect the β-cell in type 2 diabetes that appears to occur without adverse effects on the exocrine pancreas.

Neoliberalism, economic restructuring and policy change : precarious housing and precarious employment in Australia

Housing, employment and economic conditions in many nations have changed greatly over the past decades. This paper explores the ways in which changing housing markets, economic conditions and government policies have affected vulnerable individuals and households, using Australia as a case study. The paper finds a substantial number and proportion of low income Australians have been affected by housing and employment that is insecure with profound implications for vulnerability. Importantly, the paper suggests that in Australia the economic gains achieved as a consequence of mining-related growth in the early 2000s were translated as greater employment security for some on low incomes, but not all. Enhanced access to employment in this period was differentiated by gender, with women largely missing out on the growth in jobs. For the population as a whole, employment gains were offset by increased housing insecurity as accommodation costs rose. The paper finds low income lone parents were especially vulnerable because they were unable to benefit from a buoyant labour market over the decade 2000–2010. They were also adversely affected by national policy changes intended to encourage engagement with paid work. The outcomes identified for Australia are likely to have been mirrored in other nations, especially those that have embraced, or been forced to adopt, more restrictive welfare and income support regimes.

The cost-benefit of using soft silicone multilayered foam dressings to prevent sacral and heel pressure ulcers in trauma and critically ill patients: a within-trial analysis of the Border Trial.

Little is known about the cost-benefit of soft silicone foam dressings in pressure ulcer (PU) prevention among critically ill patients in the emergency department (ED) and intensive care unit (ICU). A randomised controlled trial to assess the efficacy of soft silicone foam dressings in preventing sacral and heel PUs was undertaken among 440 critically ill patients in an acute care hospital. Participants were randomly allocated either to an intervention group with prophylactic dressings applied to the sacrum and heels in the ED and changed every 3 days in the ICU or to a control group with standard PU prevention care provided during their ED and ICU stay. The results showed a significant reduction of PU incidence rates in the intervention group (P = 0·001). The intervention cost was estimated to be AU$36·61 per person based on an intention-to-treat analysis, but this was offset by lower downstream costs associated with PU treatment (AU$1103·52). Therefore, the average net cost of the intervention was lower than that of the control (AU$70·82 versus AU$144·56). We conclude that the use of soft silicone multilayered foam dressings to prevent sacral and heel PUs among critically ill patients results in cost savings in the acute care hospital.

Rigs-to-reefs: Will the deep sea benefit from artificial habitat?

As a peak in the global number of offshore oil rigs requiring decommissioning approaches, there is growing pressure for the implementation of a "rigs-to-reefs" program in the deep sea, whereby obsolete rigs are converted into artificial reefs. Such decommissioned rigs could enhance biological productivity, improve ecological connectivity, and facilitate conservation/restoration of deep-sea benthos (eg cold-water corals) by restricting access to fishing trawlers. Preliminary evidence indicates that decommissioned rigs in shallower waters can also help rebuild declining fish stocks. Conversely, potential negative impacts include physical damage to existing benthic habitats within the "drop zone", undesired changes in marine food webs, facilitation of the spread of invasive species, and release of contaminants as rigs corrode. We discuss key areas for future research and suggest alternatives to offset or minimize negative impacts. Overall, a rigs-to-reefs program may be a valid option for deep-sea benthic conservation. © The Ecological Society of America.

Defense against packet dropping attacks in opportunistic networks

Opportunistic networks (OppNets) are an interesting topic that are seen to have a promising future. Many protocols have been developed to accommodate the features of OppNets such as frequent partitions, long delays, and no end-to-end path between the source and destination nodes. Embedding security into these protocols is challenging and has taken a lot of attention in research. One of the attacks that OppNets are exposed to is the packet dropping attack, where the malicious node attempts to drop some packets and forwards an incomplete number of packets which results in the distortion of the message. To increase the security levels in OppNets, this paper presents an algorithm developed to detect packet dropping attacks, and finds the malicious node that attempted the attack. The algorithm detects the attack by using an indicative field in the header section of each packet; the indicative field has 3 sub fields - the identification field, the flag field, and the offset field. These 3 fields are used to find if a node receives the complete original number of packets from the previous node. The algorithm will have the advantage of detecting packets dropped by each intermediate node, this helps solve the difficulties of finding malicious nodes by the destination node only.

Perceptions of fairness in the psychological contracts of allied health professionals

Purpose-The purpose of this paper is to investigate the relationships between components of the psychological contract, organisational justice, and negative affectivity (NA), with key employee outcomes (i.e. organisational commitment, job satisfaction, depression, and psychological distress) among allied health professionals. Design/methodology/approach-In total, 134 (response rate of 46 per cent) Australian allied health professional completed a questionnaire. Findings-Multiple regressions revealed that higher NA was associated with lower organisational commitment, lower job satisfaction, and higher levels of depression. The psychological contract variable, breach, was associated with depression. Informational justice was associated with organisational commitment. Distributive justice was associated with job satisfaction. Research limitations/implications-This research is limited by its cross-sectional design and that the data were self-reported. The results obtained suggest the potential utility of collecting longitudinal data to replicate and extend the results. Practical implications-While NA may be beyond management control, it may be ameliorated by attention to improving communication of management decisions and by sensitivity to the elements implicit in psychological contracts. The negative consequences of contract breach may be offset by informational and distributive justice. Originality/value-This study is one of the first to examine multiple measures of the psychological contract in addition to organisational justice and NA. Further, this study adds to the literature for allied health professionals, where little is known about factors contributing to their turnover.

Loss of 'blue carbon' from coastal salt marshes following habitat disturbance

Increased recognition of the global importance of salt marshes as 'blue carbon' (C) sinks has led to concern that salt marshes could release large amounts of stored C into the atmosphere (as CO2) if they continue undergoing disturbance, thereby accelerating climate change. Empirical evidence of C release following salt marsh habitat loss due to disturbance is rare, yet such information is essential for inclusion of salt marshes in greenhouse gas emission reduction and offset schemes. Here we investigated the stability of salt marsh (Spartinaalterniflora) sediment C levels following seagrass (Thallasiatestudinum) wrack accumulation; a form of disturbance common throughout the world that removes large areas of plant biomass in salt marshes. At our study site (St Joseph Bay, Florida, USA), we recorded 296 patches (7.5 ± 2.3 m(2) mean area ± SE) of vegetation loss (aged 3-12 months) in a salt marsh meadow the size of a soccer field (7 275 m(2)). Within these disturbed patches, levels of organic C in the subsurface zone (1-5 cm depth) were ~30% lower than the surrounding undisturbed meadow. Subsequent analyses showed that the decline in subsurface C levels in disturbed patches was due to loss of below-ground plant (salt marsh) biomass, which otherwise forms the main component of the long-term 'refractory' C stock. We conclude that disturbance to salt marsh habitat due to wrack accumulation can cause significant release of below-ground C; which could shift salt marshes from C sinks to C sources, depending on the intensity and scale of disturbance. This mechanism of C release is likely to increase in the future due to sea level rise; which could increase wrack production due to increasing storminess, and will facilitate delivery of wrack into salt marsh zones due to higher and more frequent inundation.