Physiological stress responses in the warm-water fish matrinxã (Brycon amazonicus) subjected to a sudden cold shock

The present work evaluated several aspects of the generalized stress response [endocrine (cortisol), metabolic (glucose), hematologic (hematocrit and hemoglobin) and cellular (HSP70)] in the Amazonian warm-water fish matrinxã (Brycon amazonicus ) subjected to an acute cold shock. This species farming has been done in South America, and growth and feed conversion rates have been interesting. However, in subtropical areas of Brazil, where the water temperature can rapidly change, high rates of matrinxã mortality have been associated with abrupt decrease in the water temperature. Thus, we subjected matrinxã to a sudden cold shock by transferring the fish directly to tanks in which the water temperature was 10oC below the initial conditions (cold shock from 28ºC to 18oC). After 1h the fish were returned to the original tanks (28ºC). The handling associated with tank transfer was also imposed on control groups (not exposed to cold shock). While exposure to cold shock did not alter the measured physiological conditions within 1h, fish returned to the ambient condition (water at 28º C) significantly increased plasma cortisol and glucose levels. Exposure to cold shock and return to the warm water did not affect HSP70 levels. The increased plasma cortisol and glucose levels after returning the fish to warm water suggest that matrinxã requires cortisol and glucose for adaptation to increased temperature.

Triploid and diploid rainbow trout do not differ in their stress response to transportation

We examined the neuroendocrine and cellular stress responses of diploid and triploid rainbow trout Oncorhynchus mykiss to transportation. Juvenile diploid and triploid rainbow trout (28 and 26 g/fish average weight, respectively) were stocked at 100 g/L in replicate 70-L tanks and subjected to transportation for an 8-h period. Subsequent levels of plasma cortisol and glucose and of cellular hepatic glutathione (GSH) and heat shock protein 70 (Hsp70) were similar between ploidy groups, indicating that triploid fish respond to transportation in much the same way as diploid fish. A stationary treatment was also included that involved confinement of experimental fish in similar tanks without transport to determine to what extent high-density containment contributed to the stress response in the absence of the noise and vibration of transport. Unexpectedly, fish in the stationary treatment had significantly higher plasma cortisol and glucose levels than the transported fish; however, this might be attributable to a confounding effect of hyperoxia, as oxygen levels fluctuated between 150% and 460% saturation in the stationary tank, while those in the transported tank remained within 100–200% saturation. We suggest that when long stops are necessary while transporting fish, water agitators be used to preclude the additional stress of excessive gas saturation. This may be particularly important for triploid fish, which had lower hepatic GSH levels than diploid fish as well as a low level of mortality in the stationary treatment, unlike the diploid fish.

Urban informality in the developing world - Opportunistic urban design processes and prototypes for the developed world

The turn of the twenty first century heralds a fundamental shift in the world’s population towards cities in what has been termed The Urban Age, most visible in the developing world. Instead of the dichotomy of ‘First World models’ and ‘Third world problems’, research has begun to explore the complexities of urban informality revealing new approaches to the complex creation of urban spaces. This paper will analyse the prevailing perspectives in order to develop an understanding of informality as an urban evolution through necessity. Finally through an analysis of professional urban design experiments the paper highlights opportunities and challenges within the developing world for new and exciting urban design processes in the developed world.

The War Memoirs: Some origins of the thought of W.R. Bion

The work of W. R. Bion changed the shape of psychoanalytic theory in fundamental ways, one of the most important of which was Bion's insight into the nature of normal projective identification. No other psychoanalytic theorist has Bion's ability to represent the horrors of psychic abandonment and the converse, the absolute necessity of the presence of another mind for psychic survival. Through a discussion of Bion's War Memoirs 1917-1919 (Bion, 1997), Attacks on linking and A theory of thinking (1993), this paper explores the link between war, masculinity, the maternal and Bion's sensitivity to the significance of everyday interpersonal contact. It is argued that Bion's apocalyptic experiences as a teenage tank commander gave him shattering insight into the extent to which mind is inter-mind, self is inter-self. Bion's life writing has the quality of survivor insight: 'And only I am escaped alone to tell thee' (Job 1: 14-19), as he returns repeatedly to the events of the day when he 'died ', 8 August 1918. His insight into the elemental passions nature of love, hate and mindlessness are borne of his experiences on the battlefield, and exquisitely crystallized in his repeated explorations of an encounter with a dying soldier.

Flagging authenticity

How important are national flags in the emerging global society? This is a relevant question for a world still coming to terms with post-colonialism while also having to deal with the economic and cultural side of globalisation. This contribution to The Identity Journal looks at the value of authenticity and its importance in redefining Australia's national identity. The role of Indigenous culture and its relationship to a broad national identity is explored in the context of proposed changes to the Australian constitution. If passed this constitutional amendment will formally recognise Australia as the home of the worlds oldest living cultures. 

Sharing questions and initiatives, the professional organizations' point of view

The Book Designer: Careers and Professionalisation features contrubutions from 62 International design educators and professionals.  These leaders in their field provide analysis and expert commontary on the skill and epertise now required for sucessful career in design.

Chassis for pneumatic vehicle

A pneumatic vehicle is provided with a first sub-assembly with a chassis, part of the vehicle body, a pair of B-pillars, a pair of rear rails, wheels, an elongate aluminum compressed load bearing air tank oriented longitudinally in the chassis, side panels connected to the tank and the wheels, a heat exchanger to heat the compressed air, and an air motor driven by the heated, compressed air and connected to a wheel. A ventilation system has a restrictive solenoid valve for directing air to the heat exchanger. The air tank is provided with a carbon filament reinforced plastic layer, and a fiberglass and aramid-fiber layer. A second sub-assembly includes part of the vehicle body bonded to the first-sub-assembly using a structural adhesive, a pair of A-pillars, and a pair of roof rails. Seating includes inflatable components for adjustment.

Pneumatic powertrain for an automotive vehicle

A pneumatic vehicle is provided with a chassis, wheels, a compressed air tank, a heat exchanger to heat the compressed air, and an air motor driven by the heated air and connected to at least one wheel. A pneumatic vehicle is provided with a chassis, wheels, a compressed air tank, and an air motor driven by the compressed air and connected to a wheel. The vehicle also has a ventilation system for the passenger compartment, a heat exchanger, and a restrictive solenoid valve for directing ventilation system air to the heat exchanger. A pneumatic vehicle is provided with a chassis, wheels, an aluminum compressed air tank, a carbon filament reinforced plastic layer over the tank, a fiberglass and aramid-fiber layer over the carbon filament reinforced plastic layer, and an air motor driven by the compressed air and connected to at least one wheel.

Body for pneumatic vehicle

A pneumatic vehicle is provided with a first and second sub-assembly. The first sub-assembly has a chassis supporting an air tank. The second sub-assembly has part of the vehicle body and is bonded to the first-sub-assembly using a structural adhesive. Another pneumatic vehicle is provided with a first and second sub-assembly. The first sub-assembly has a chassis, air tank, part of the vehicle body, a pair of B-pillars, and a pair of rear rails. The second sub-assembly has a part of the vehicle body, a pair of A-pillars, and a pair of roof rails. A vehicle seat is provided with a seat base and upright seat back providing a seating area. A bladder located in the central region of the seating area inflates to provide two bucket seats, and deflates to provide a bench seat. The vehicle seat also has inflatable inserts to provide cushioning.

Dietary n-6/n-3 LC-PUFA ratio, temperature and time interactions on nutrients and fatty acids digestibility in Atlantic salmon

The objective of the present study was to evaluate the effects of altered dietary n-3/n-6 LC-PUFA ratio, adaptation to diet over time, different water temperatures, and their interactions on nutrients and fatty acids digestibility in juvenile Atlantic salmon. Three experimental diets were formulated to be identical, with the only exception of the ratio of eicosapentaenoic acid (EPA, 20:5n-3) to arachidonic acid (ARA, 20:4n-6), and fed to triplicate groups of juvenile Atlantic salmon (Salmo salar) of 55. g initial body weight. Fish were reared in a fully controlled recirculating aquaculture system, fed to apparent satiety twice daily and kept at 10. °C and for an initial period of 100. days, and faeces were collected for digestibility estimation. Then, half of the fish of each experimental tank were moved to a separate system, where the water temperature was gradually increased up to 20. °C. Fish were maintained in the two systems for an additional period of 50. days, and faeces were collected for digestibility estimation from both groups of fish at the two water temperatures. This study concluded that dietary treatments and time had only minor effects, whereas environmental temperature resulted in modified digestibility values, with increased nutrient digestibility with increasing temperature. Varying EPA/ARA ratio in the diet had only minor direct effects on digestibility, with no direct effect on overall nutrients digestibility, and fundamentally only statistically significant effects in the fatty acid digestibility of EPA and ARA themselves. Because of current increasing pressure for more efficient fish oil replacement strategies, increasing interest in dietary ARA in aquafeed and increasing relevance and occurrence of sub-optimal rearing temperature in commercial aquaculture, this study can be considered to be important as it provided a series of fundamental information, which are envisaged to be useful towards addressing these constraints and possible nutritional remedial strategies.

Chassis for pneumatic vehicle

A pneumatic vehicle is provided with a first sub-assembly with a chassis, part of the vehicle body, a pair of B-pillars, a pair of rear rails, wheels, an elongate aluminum compressed load bearing air tank oriented longitudinally in the chassis, side panels connected to the tank and the wheels, a heat exchanger to heat the compressed air, and an air motor driven by the heated, compressed air and connected to a wheel. A ventilation system has a restrictive solenoid valve for directing air to the heat exchanger. The air tank is provided with a carbon filament reinforced plastic layer, and a fiberglass and aramid-fiber layer. A second sub-assembly includes part of the vehicle body bonded to the first-sub-assembly using a structural adhesive, a pair of A-pillars, and a pair of roof rails. Seating includes inflatable components for adjustment.