33 resultados para CERIUM OXIDES
Resumo:
With an increase in use of nanoparticles (NPs) in day to day products, these particles eventually enter the wastewater treatment plant and get removed from the effluent while getting accumulated in the sludge at ever increasing concentrations. These NPs have a potential for causing inhibition in sludge digestion processes. Therefore, this research focused on the effects of cerium (IV) oxide (CeO
Resumo:
Lipases, which can be immobilized and reused for many reaction cycles, are important enzymes with many industrial applications. A key challenge in lipase immobilization for catalysis is to open the lipase lid and maintain it in an open conformation in order to expose its active site. Here we have designed "tailor-made" graphene-based nanosupports for effective lipase (QLM) immobilization through molecular engineering, which is in general a grand challenge to control biophysicochemical interactions at the nano-bio interface. It was observed that increasing hydrophobic surface increased lipase activity due to opening of the helical lid present on lipase. The molecular mechanism of lid opening revealed in molecular dynamics simulations highlights the role of hydrophobic interactions at the interface. We demonstrated that the open and active form of lipase can be achieved and tuned with an optimized activity through chemical reduction of graphene oxide. This research is a major step toward designing nanomaterials as a platform for enhancing enzyme immobilization/activity.
Resumo:
The thesis focuses on use of carbon nanoparticles, namely graphene oxides for studying the effect of hydrophobicity on enzyme structure and how they it influences the enzyme activity at molecular level. It was observed that controlling the hydrophobicity of the nanoparticle is a key towards modulating enzyme activity.