86 resultados para APTAMER-BASED SENSORS


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Vision-based tracking sensors typically provide nonlinear measurements
of the targets Cartesian position and velocity state components. In this paper we derive linear measurements using an analytical measurement conversion technique which can be used with two (or more) vision sensors. We derive
linear measurements in the target’s Cartesian position and velocity components and we derive a robust version of a linear Kalman filter. We show that our linear robust filter significantly outperforms the extended Kalman Filter. Moreover, we prove that the state estimation error is bounded.

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This mini review highlights the synthesis and photophysical evaluation of anion sensors, for nonaqueous solutions, that have been developed in our laboratories over the last few years. We have focused our research mainly on developing fluorescent photoinduced electron transfer (PET) sensors based on the fluorophore-spacer-anion receptor principle using several anthracene (emitting in the blue) and 1,8-naphthalimide (emitting in the green) fluorophores, with the aim of targeting biologically and industrially relevant anions such as acetates, phosphate and amino acids, as well as halides such as fluoride. The receptors and the fluorophore are separated by a short methyl or ethyl spacer, where the charge neutral anion receptors are either aliphatic or aromatic urea (or thiourea) moieties. For these, the anion recognition is through hydrogen bonding, yielding anion:receptor complexes. Such bonding gives rise to enhanced reduction potential in the receptor moieties which causes enhancement in the rate of PET quenching of the fluorophore excited state from the anion:receptor moiety. This design can be further elaborated on by incorporating either two fluorophores, or urea/thiourea receptors into the sensor structures, using anthracene as a fluorophore. For the latter design, the sensors were designed to achieve sensing of bis-anions, such as di-carboxylates or pyrophosphate, where the anion bridged the anthracene moiety. In the case of the naphthalimide based mono-receptor based PET sensors, it was discovered that in DMSO the sensors were also susceptible to deprotonation by anions such as F− at high concentrations. This led to substantial changes in the absorption spectra of these sensors, where the solution changed colour from yellow/green to deep blue, which was clearly visible to the naked eye. Hence, some of the examples presented can act as dual fluorescent-colorimetric sensors for anions. Further investigations into this phenomenon led to the development of simple colorimetric sensors for fluorides, which upon exposure to air, were shown to fix carbon dioxide as bicarbonate.

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This review article focuses primarily on the work carried in our laboratories over the last few years using luminescent and colorimetric sensors, where the anion recognition occurs through hydrogen bonding in organic or aqueous solvents. This review begins with the story of the discovery of fluorescent photoinduced electron transfer (PET) sensors for anions using charged neutral urea or thiourea receptors where both fluorescent and NMR spectroscopic methods monitored anion recognition. This work led to the development of dual luminescent and colorimetric anion sensors based on the use of the ICT based naphthalimide chromophore, where ions such as fluoride gave rise to changes in both the fluorescence and the absorption spectra of the sensors, but at different concentrations. Here, the former changes were due to hydrogen bonding interactions, whereas the latter was due to the deprotonation of acidic protons, giving rise to the formation of the bifluoride anion (HF2−). Modification of the 4-amino-l,8-naphthalimide moiety has facilitated the formation of colorimetric anion sensors that work both in organic or aqueous solutions. Such charge neutral receptor motifs have also been incorporated into organic scaffolds with norbomyl and calixarene backbones, which have enabled us to produce anion directed self-assembled structures.

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In this paper we examine the geometrically constrained optimization approach to localization with hybrid bearing (angle of arrival, AOA) and time difference of  arrival (TDOA) sensors. In particular, we formulate a constraint on the measurement errors which is then used along with constraint-based optimization tools in order to estimate the maximum likelihood values of the errors given an appropriate cost function. In particular we focus on deriving a localization algorithm for stationary target localization in the so-called adverse localization geometries where the relative positioning of the sensors and the target do not readily permit accurate or convergent localization using traditional approaches. We illustrate this point via simulation and we compare our approach to a number of different techniques that are discussed in the literature.

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A novel technique is here presented, based on inverse opal metal oxide structures for the production of high quality macro and meso-porous structures for gas sensing. Taking advantage of a sol-gel templated approach. different mixed semiconducting oxides with high surface area, commonly used in chemical sensing application, were synthesized. In this work we report the
comparison between SnO2 and SnO2:Zn. As witnessed by Scanning and Transmission Electron Microscopy (SEM and TEM) analyses and by Powder x-ray Diffraction (PX RD), highly ordered meso-porous structures were formed with oxide crystalline size never exceeding 20 nm . The filled templates. in form of thick films, were bound to allumina substrate with Pt interdigitated contacts
and Pt heater, through in situ calcination, in order to perform standard electrical characterization. Pollutant gases like CO and NO2 and methanol. as interfering gas, were used for the targeted electrical gas tests. All samples showed low detection limits towards both reducing and oxidizing species in low temperature measurements. Moreover, the addition of high molar percentages of Zn( II) affected the beha viour of electrical response improv ing the se lecti vity of the proposed system.

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Recently, Aissa-El-Bey et al. have proposed two subspacebased methods for underdetermined blind source separation (UBSS) in time-frequency (TF) domain. These methods allow multiple active sources at TF points so long as the number of active sources at any TF point is strictly less than the number of sensors, and the column vectors of the mixing matrix are pairwise linearly independent. In this correspondence, we first show that the subspace-based methods must also satisfy the condition that any M × M submatrix of the mixing matrix is of full rank. Then we present a new UBSS approach which only requires that the number of active sources at any TF point does not exceed that of sensors. An algorithm is proposed to perform the UBSS.

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Wireless sensor networks (WSNs) are proposed as powerful means for fine grained monitoring in different classes of applications at very low cost and for extended periods of time. Among various solutions, supporting WSNs with intelligent mobile platforms for handling the data management, proved its benefits towards extending the network lifetime and enhancing its performance. The mobility model applied highly affects the data latency in the network as well as the sensors’ energy consumption levels. Intelligent-based models taking into consideration the network runtime conditions are adopted to overcome such problems. In this chapter, existing proposals that use intelligent mobility for managing the data in WSNs are surveyed. Different classifications are presented through the chapter to give a complete view on the solutions lying in this domain. Furthermore, these models are compared considering various metrics and design goals.

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This paper investigates the linear separation requirements for Angle-of-Arrival (AoA) sensors, in order to achieve the optimal performance in estimating the position of a target from multiple and typically noisy sensor measurements. We analyse the sensor-target geometry in terms of the Cramer-Rao inequality and the corresponding Fisher information matrix, in order to characterize localization performance with respect to the linear spacial distribution. Here we consider a situation where one sensor is fixed and the rest are free to be positioned in a linear array.

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This paper investigates the problem of location and velocity detection of a mobile agent using Received Signal Strength (RSS) measurements captured by geographically distributed seed nodes. With inherently nonlinear power measurements, we derive a powerful linear measurement scheme using an analytical measurement conversion technique which can readily be used with RSS measuring sensors. We also employ the concept of sensor fusion in conjunction for the case of redundant measurements to further enhance the estimation accuracy.

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In this paper, we examine the optimal linear separation requirements for AoA sensors, in order to achieve the best performance in estimating the position of a target subjected to noisy measurements. Cramer-Rao inequality and the corresponding Fisher information matrix are used to analyze the sensor-target geometry, in order to characterize localization performance with respect to the linear spacial distribution of sensors.

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Wireless sensor networks (WSNs) are attractive for monitoring and gathering physical information (e.g. temperature) via lots of deployed sensors. For the applications in WSNs, Web service is one of the recommended frameworks to publish, invoke, and manage services. However, the standard Web service description language (WSDL), defines only the service input and output while ignoring the corresponding input-to-output mapping relationships. This presents a serious challenge in distinguishing services with similar input and output interface. In this paper, we address this challenge by embedding the service policy into the traditional WSDL2.0 schema to describe the input-to-output mapping relationships. The service policy is then transformed into a policy binary tree so that the similarity between different Web services can be quantitatively evaluated. Furthermore, a new service redundancy detection approach is proposed based on this similarity. Finally, the case study and experimental analysis illustrate the applicability and capability of the proposed service redundancy detection approach.

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To overcome the interference of acetone when detecting alcohol, a novel alcohol detector based on zirconia-doped SnO2 nanofibers were fabricated through electrospinning technique and calcination process. The samples have been characterized by scanning electron microscopy, transmission electron microscopy, X-ray diffraction, X-ray photoelectron spectroscopy, and their gas sensing properties have also been investigated. When exposed to alcohol vapor, the nanofibers containing 15 mol% zirconia exhibit the best sensing properties. Moreover, the sensor holds the successful discrimination between acetone and alcohol, which makes our product a good candidate in fabricating highly selective sensors in practice.

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This thesis presents the design, modelling, fabrication, and biological evaluation of a microcantilever-based aptasensor. It is the first reported work on aptasensors with aptamer immobilized on a bare SU-8 surface. Aptasensor surface funtionalisation was achieved using gas plasma treatment. Label-free detection of thrombin molecules using the aptasensor was successfully demonstrated.

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This paper investigates the linear separation requirements for range sensors in order to achieve the optimal performance in estimating the position of a target from multiple and typically noisy sensor measurements. We analyze the sensor-target geometry in terms of the Cramer-Rao inequality and the corresponding Fisher information matrix, in order to characterize localization performance with respect to the linear special distribution.

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Since Guided wave (GW) is sensitive to small damage and can propagate a relatively longer distance with relatively less attenuation, GW-based method has been found as an effective and efficient way to detect incipient damages. In this study, a full-scale concrete joint was constructed to further verify the effectiveness of GW-based method on real civil structures. GW tests were conducted in three stages, including baseline, serviceability and damage conditions. The waves are excited by one actuator and received by several sensors, which are made up of independent piezoelectric elements. Experimental results show that the mehod is promising for damage identification in practices.