Medial prefrontal cortex control of the paraventricular hypothalamic nucleus response to psychological stress : possible role of the bed nucleus of the stria terminalis

The medial prefrontal cortex (mPFC) has been strongly implicated in control of the paraventricular nucleus of the hypothalamus (PVN) response to stress. Because of the paucity of direct projections from the mPFC to the PVN, we sought to investigate possible brain regions that might act as a relay between the two during psychological stress. Bilateral ibotenic acid lesions of the rat mPFC enhanced the number of Fos-immunoreactive cells seen in the PVN after exposure to the psychological stressor, air puff. Altered neuronal recruitment was seen in only one of the candidate relay populations examined, the ventral bed nucleus of the stria terminalis (vBNST). Furthermore, bilateral ibotenic acid lesions of the BNST caused a significant attenuation of the PVN response to air puff. To better characterize the structural relationships between the mPFC and PVN, retrograde tracing studies were conducted examining Fos expression in cells retrogradely labeled with cholera toxin b subunit (CTb) from the PVN and the BNST. Results obtained were consistent with an important role for both the mPFC and BNST in the mpPVN CRF cell response to air puff. We suggest a set of connections whereby a direct PVN projection from the ipsilateral vBNST is involved in the mpPVN response to air puff and this may, in turn, be modulated by an indirect projection from the mPFC to the BNST.

Medial prefrontal cortex suppression of the hypothalamic–pituitary–adrenal axis response to a physical stressor, systemic delivery of interleukin-1β

Previous studies have shown that the medial prefrontal cortex can suppress the hypothalamic–pituitary–adrenal axis response to stress. However, this effect appears to vary with the type of stressor. Furthermore, the absence of direct projections between the medial prefrontal cortex and corticotropin-releasing factor cells at the apex of the hypothalamic–pituitary–adrenal axis suggest that other brain regions must act as a relay when this inhibitory mechanism is activated. In the present study, we first established that electrolytic lesions involving the prelimbic and infralimbic medial prefrontal cortex increased plasma adrenocorticotropic hormone levels seen in response to a physical stressor, the systemic delivery of interleukin-1β. However, medial prefrontal cortex lesions did not alter plasma adrenocorticotropic hormone levels seen in response to a psychological stressor, noise. To identify brain regions that might mediate the effect of medial prefrontal cortex lesions on hypothalamic–pituitary–adrenal axis responses to systemic interleukin-1β, we next mapped the effects of similar lesions on interleukin-1β-induced Fos expression in regions previously shown to regulate the hypothalamic–pituitary–adrenal axis response to this stressor. It was found that medial prefrontal cortex lesions reduced the number of Fos-positive cells in the ventral aspect of the bed nucleus of the stria terminalis. However, the final experiment, which involved combining retrograde tracing with Fos immunolabelling, revealed that bed nucleus of the stria terminalis-projecting medial prefrontal cortex neurons were largely separate from medial prefrontal cortex neurons recruited by systemic interleukin-1β, an outcome that is difficult to reconcile with a simple medial prefrontal cortex–bed nucleus of the stria terminalis–corticotropin-releasing factor cell control circuit.

Opposing roles for medial and central amygdala in the initiation of noradrenergic cell responses to a psychological stressor

Psychological stressors trigger the activation of medullary noradrenergic cells, an effect that has been shown to depend upon yet-to-be-identified structures located higher in the brain. To test whether the amygdala is important in this regard, we examined the effects of amygdala lesions on noradrenergic cell responses to restraint, and also looked at whether any amygdala cells that respond to restraint project directly to the medulla. Ibotenic acid lesions of the medial amygdala completely abolished restraint-induced Fos expression in A1 and A2 noradrenergic cells. In contrast, lesions of the central amygdala actually facilitated noradrenergic cell responses to restraint. Tracer deposits in the dorsomedial (but not ventrolateral) medulla retrogradely labelled many cells in the central nucleus of the amygdala, but none of these cells expressed Fos in response to restraint. These data suggest for the first time that the medial amygdala is critical to the activation of medullary noradrenergic cells by a psychological stressor whereas the central nucleus exerts an opposing, inhibitory influence upon noradrenergic cell recruitment. The initiation of noradrenergic cell responses by the medial amygdala does not involve a direct projection to the medulla. Accordingly, a relay through some other structure, such as the hypothalamic paraventricular nucleus, warrants careful consideration.

Hacking the undernet : libertarian limits, commercial containment

In this article, we explare a recent incident involving aprolonged and severe denial of service attack directed at the Undemet Intemet Relay Chat network. It put the future viabifity of Undernet in doubt; it took some months for service quality to be restored. The circumstances of the attack and the responses, both technical and social, within Undemet are enlightening in themselves, as we discuss. But they also allow us to explore, contrast, and match up the limits of the libertarianism that seems embedded in the socio-technics of the Intemet and the possible and actual containment of 'free' services in a 'free' market, through the operation of commercial transactions.

Trajectories of desire in Genet and Wittig

At their most extreme Jean Genet and Monique Wittig stage revolutionary desire through their practice of language as material, embodied productivity in scenographies that decentre the ‘human' through a volatile mix of semiotic violence and iconoclastic eroticism. In fact, both writers strategically universalise homoerotic desire, plotting its trajectories to disrupt, deconstruct, or explode in parodic hilarity the cultural practices subtending patriarchal imperialism. This paper will be looking at the ceaseless productivity of desire in their texts as it moves through abjection and animal-becoming and finds serial ignition throughout its metonymic relay, arousing obstruction itself as its medium. Evidently these tropes and the concept of desire as productive come from Deleuze and Guattari (Deleuze & Gauttari 2008a; 2008b). While these subversive poetics are inseparable from the project to storm and overthrow what Timothy Mathews (Mathews 2000) calls the ‘image-fortresses' of patriarchal imperialism, they are less about utopian arrival than about the endlessly renewed performance of desire as metamorphic.

When surfacers do not dive : multiple significance of extended surface times in marine turtles

Marine turtles spend more than 90% of their life underwater and have been termed surfacers as opposed to divers. Nonetheless turtles have been reported occasionally to float motionless at the surface but the reasons for this behaviour are not clear. We investigated the location, timing and duration of extended surface times (ESTs) in 10 free-ranging loggerhead turtles (Caretta caretta) and the possible relationship to water temperature and diving activity recorded via satellite relay data loggers for 101–450 days. For one turtle that dived only in offshore areas, ESTs contributed 12% of the time whereas for the other turtles ESTs contributed 0.4–1.8% of the time. ESTs lasted on average 90 min but were mostly infrequent and irregular, excluding the involvement of a fundamental regulatory function. However, 82% of the ESTs occurred during daylight, mostly around noon, suggesting a dependence on solar radiation. For three turtles, there was an appreciable (7°C to 10.5°C) temperature decrease with depth for dives during periods when ESTs occurred frequently, suggesting a re-warming function of EST to compensate for decreased body temperatures, possibly to enhance digestive efficiency. A positive correlation between body mass and EST duration supported this explanation. By contrast, night-active turtles that exceeded their calculated aerobic dive limits in 7.6–16% of the dives engaged in nocturnal ESTs, probably for lactate clearance. This is the first evidence that loggerhead turtles may refrain from diving for at least two reasons, either to absorb solar radiation or to recover from anaerobic activity.

The role of infrequent and extraordinary deep dives in leatherback turtles (Dermochelys coriacea)

Infrequent and exceptional behaviours can provide insight into the ecology and physiology of a particular species. Here we examined extraordinarily deep (300–1250 m) and protracted (>1h) dives made by critically endangered leatherback turtles (Dermochelys coriacea) in the context of three previously suggested hypotheses: predator evasion, thermoregulation and exploration for gelatinous prey. Data were obtained via satellite relay data loggers attached to adult turtles at nesting beaches (N=11) and temperate foraging grounds (N=2), constituting a combined tracking period of 9.6 years (N=26,146 dives) and spanning the entire North Atlantic Ocean. Of the dives, 99.6% (N=26,051) were to depths <300 m with only 0.4% (N=95) extending to greater depths (subsequently termed `deep dives'). Analysis suggested that deep dives: (1) were normally distributed around midday; (2) may exceed the inferred aerobic dive limit for the species; (3) displayed slow vertical descent rates and protracted durations; (4) were much deeper than the thermocline; and (5) occurred predominantly during transit, yet ceased once seasonal residence on foraging grounds began. These findings support the hypothesis that deep dives are periodically employed to survey the water column for diurnally descending gelatinous prey. If a suitable patch is encountered then the turtle may cease transit and remain within that area, waiting for prey to approach the surface at night. If unsuccessful, then migration may continue until a more suitable site is encountered. Additional studies using a meta-analytical approach are nonetheless recommended to further resolve this matter.

Overwintering behaviour in sea turtles : dormancy is optional

Thirteen loggerhead turtles Caretta caretta were released (10 from Naples, Italy, 2 from Monastir, Tunisia, 1 from Gallipoli, South Italy) with satellite relay data loggers (SRDL) to elucidate their overwintering behaviour. Nine turtles were successfully tracked throughout the winter, while 4 SRDLs failed to transmit after short deployment periods. Of these 9, 4 remained within 80 km of the release site, 3 travelled to a distant overwintering site, and 2 continued to move and did not remain within 80 km of a specific site. Apart from these differences, all turtles stayed near the coast and dedicated most of their time to dives lasting 3 h and longer. Maximum dive durations ranged from 270 to 480 min and were highly correlated with water temperatures, which fell below the supposed 15°C threshold for sea turtle hibernation in all overwintering sites. Median dive depths were between 4 and 24 m and were, thus, well within the mixed layer, as revealed by temperature profiles, which also were relayed by the SRDLs. No evidence was found that the turtles preferred warmer temperatures to overwinter in, because the range of temperature was very narrow on both the horizontal and the vertical scale of their movements. Despite the long resting phases and the low temperatures (minimum = 11.8°C) all turtles retained activity to some degree, at least to commute between the depth of resting and the surface to breathe. While the degree of winter dormancy is certainly affected by temperature, turtles were by no means obligatory hibernators, and their ability to move and even forage during the winter may be important for their growth and maturation rates, as well as their reproductive output.

First records of dive durations for a hibernating sea turtle

The first published record, from the early 1970s, of hibernation in sea turtles is based on the reports of the indigenous Indians and fishermen from Mexico, who hunted dormant green turtles (Chelonia mydas) in the Gulf of California. However, there were no successful attempts to investigate the biology of this particular behaviour further. Hence, data such as the exact duration and energetic requirements of dormant winter submergences are lacking. We used new satellite relay data loggers to obtain the first records of up to 7 h long dives of a loggerhead turtle (Caretta caretta) overwintering in Greek waters. These represent the longest dives ever reported for a diving marine vertebrate. There is strong evidence that the dives were aerobic, because the turtle surfaced only for short intervals and before the calculated oxygen stores were depleted. This evidence suggests that the common belief that sea turtles hibernate underwater, as some freshwater turtles do, is incorrect.

Knowledge management in future factories

Technology in the manufacturing sector has seen rapid change, transforming from stand alone, manual processes to smart, integrated systems. We have witnessed the migration of relay-based systems to advance SCADA systems, manual pro-duction to fully automated, and hand written reports to interactive computer-based dashboards. We are now seeing the emergence of smart products manufactured in smart plants and the evolution of smart services in manufacturing. Future manu-facturing systems will be distinguished by intelligent machines, automation and human factors’ integration. This talk will focus on how knowledge can be embed-ded in processes and products through the use of simulation and modelling tools to streamline future smart production systems and improve product quality. The implications to future smart manufacturing enterprises are explored through a se-ries of case studies from aerospace, mining and small and medium manufacturing enterprises.

Medication communication through documentation in medical wards: knowledge and power relations

Health professionals communicate with each other about medication information using different forms of documentation. This article explores knowledge and power relations surrounding medication information exchanged through documentation among nurses, doctors and pharmacists. Ethnographic fieldwork was conducted in 2010 in two medical wards of a metropolitan hospital in Australia. Data collection methods included participant observations, field interviews, video-recordings, document retrieval and video reflexive focus groups. A critical discourse analytic framework was used to guide data analysis. The written medication chart was the main means of communicating medication decisions from doctors to nurses as compared to verbal communication. Nurses positioned themselves as auditors of the medication chart and scrutinised medical prescribing to maintain the discourse of patient safety. Pharmacists utilised the discourse of scientific judgement to guide their decision-making on the necessity of verbal communication with nurses and doctors. Targeted interdisciplinary meetings involving nurses, doctors and pharmacists should be organised in ward settings to discuss the importance of having documented medication information conveyed verbally across different disciplines. Health professionals should be encouraged to proactively seek out each other to relay changes in medication regimens and treatment goals.

Agent-based smart grid protection and security

The vision of a smart grid is to provide a modern, resilient, and secure electric power grid as it boasts up with a highly reliable and efficient environment through effective use of its information and communication technology (ICT). Generally, the control and operation of a smart grid which integrate the distributed energy resources (DERs) such as, wind power, solar power, energy storage, etc., largely depends on a complex network of computers, softwares, and communication infrastructure superimposed on its physical grid architecture facilitated with the deployment of intelligent decision support system applications. In recent years, multi-agent system (MAS) has been well investigated for wide area power system applications and specially gained a significant attention in smart grid protection and security due to its distributed characteristics. In this chapter, a MAS framework for smart grid protection relay coordination is proposed, which consists of a number of intelligent autonomous agents each of which are embedded with the protection relays. Each agent has its own thread of control that provides it with a capability to operate the circuit breakers (CBs) using the critical clearing time (CCT) information as well as communicate with each other through high speed communication network. Besides physical failure, since smart grid highly depends on communication infrastructure, it is vulnerable to several cyber threats on its information and communication channel. An attacker who has knowledge about a certain smart grid communication framework can easily compromise its appliances and components by corrupting the information which may destabilize a system results a widespread blackout. To mitigate such risk of cyber attacks, a few innovative counter measuring techniques are discussed in this chapter.

A multi-agent approach for enhancing transient stability of smart grids

Transient stability, an important issue to avoid the loss of synchronous operation in power systems, can be achieved through proper coordination and operation of protective devices within the critical clearing time (CCT). In view of this, the development of an intelligent decision support system is useful for providing better protection relay coordination. This paper presents an intelligent distributed agent-based scheme to enhance the transient stability of smart grids in light of CCT where a multi-agent framework (MAF) is developed and the agents are represented in such a way that they are equipped with protection relays (PRs). In addition to this, an algorithm is developed which assists the agents to make autonomous decision for controlling circuit breakers (CBs) independently. The proposed agents are responsible for the coordination of protection devices which is done through the precise detection and isolation of faults within the CCT. The agents also perform the duty of reclosing CBs after the clearance of faults. The performance of the proposed approach is demonstrated on a standard IEEE 39-bus test system by considering short-circuit faults at different locations under various load conditions. To further validate the suitability of the proposed scheme a benchmark 16-machine 68-bus power system is also considered. Simulation results show that MAF exhibits full flexibility to adapt the changes in system configurations and increase the stability margin for both test systems.

A decentralised multi-agent approach to enhance the stability of smart microgrids with renewable energy

This paper presents the impact of large penetration of wind power on the transient stability through a dynamic evaluation of the critical clearing times (CCTs) by using intelligent agent-based approach. A decentralised multi-agent-based framework is developed, where agents represent a number of physical device models to form a complex infrastructure for computation and communication. They enable the dynamic flow of information and energy for the interaction between the physical processes and their activities. These agents dynamically adapt online measurements and use the CCT information for relay coordination to improve the transient stability of power systems. Simulations are carried out on a smart microgrid system for faults at increasing wind power penetration levels and the improvement in transient stability using the proposed agent-based framework is demonstrated.

Stability analysis and resource allocation for space-based multi-access systems

In space-based networks, the data relay satellites can assist low-earth-orbit satellites in relaying data to other satellites or the ground station and improve the real time system throughput. To take full advantage of transmission resource of the cooperative relays, this paper proposes a multiple access and resource allocation strategy, in which relays can receive and transmit simultaneously according to channel characteristics of space-based systems. Based on the queueing theoretic formulation, the stability of the proposed protocol is analyzed and the maximum stable throughput region is derived, which would provide the appropriate guidance for the design of the system optimal control. Simulation results exhibit multiple factors that affect the stable throughput and verify the theoretical analysis.