HIV-1 Infection of T cells and macrophages are differentially modulated by virion-associated Hck: a nef-dependent phenomenon

The proline repeat motif (PxxP) of Nef is required for interaction with the SH3 domains of macrophage-specific Src kinase Hck. However, the implication of this interaction for viral replication and infectivity in macrophages and T lymphocytes remains unclear. Experiments in HIV-1 infected macrophages confirmed the presence of a Nef:Hck complex which was dependent on the Nef proline repeat motif. The proline repeat motif of Nef also enhanced both HIV-1 infection and replication in macrophages, and was required for incorporation of Hck into viral particles. Unexpectedly, wild-type Hck inhibited infection of macrophages, but Hck was shown to enhance infection of primary T lymphocytes. These results indicate that the interaction between Nef and Hck is important for Nef-dependent modulation of viral infectivity. Hck-dependent enhancement of HIV-1 infection of T cells suggests that Nef-Hck interaction may contribute to the spread of HIV-1 infection from macrophages to T cells by modulating events in the producer cell, virion and target cell.

The monsoon wedding phenomenon: understanding Indian students studying in Australian universities

How does the University sector identify and support the diverse needs of Indian students? This paper reports on a research project carried out on undergraduate students from India enrolled at a Melbourne‐based University. The focus is the need to understand why Indian students choose an overseas destination for tertiary study. The intent is to explore how the curriculum that they have experienced in their country prepares them for study in another. We examine the expectations of students in relation to studying overseas. The suggestion underlying this paper is that if academic and support staff in tertiary education understand international students in cultural cohorts, then it is more likely that their transition to tertiary education will be easier. We envisage that this may also lead to a greater retention rate for universities.

Migrants as Homo economicus : explaining the emerging phenomenon of a shortage of migrant labor in China's coastal provinces

The problem of a shortage of migrant labor is a new development in China's coastal provinces. We discuss the reasons for this emerging phenomenon using a conceptual framework that extends the traditional Lewis dualistic labor market model to incorporate a migrant labor market. We emphasize that migrant labor shortage in China not only reflects a declining wage gap between what peasants receive and what migrants can earn in the cities, but also the institutional legacies of the planning era such as the hukou (household registration) system which discriminates against migrants vis-a-vis urban residents in terms of access to social insurance and other social services. We proceed to draw on a unique survey of migrants and urban residents collected in Jiangsu to show that migrants receive lower incomes, and they have poorer access to social insurance than those with an urban registration in China's cities. Our findings have important implications for the alleviation of the migrant labor shortage problem.

A virtuous circle: the positive evaluation phenomenon in arts audience research

Arts impact evaluation is the subject of widespread criticism, ranging from a detailed critique of methodology to a wholesale rejection of the very purpose of the endeavour. In particular, it is often identified that audience evaluations are almost always positive. Yet whatever the critique, arts impact evaluation is becoming more and not less prevalent as a condition of public and philanthropic funding. This article explores both the methodologicaland conceptual problems that contribute to the perceived positive characterof social impact research, in two parts: (1) an investigation of the critical literature on audience evaluation, particularly in relation to the argument that evaluation is inevitably positive and as such leads to a confusion between the goals of evaluation and arts advocacy; (2) a reflection on our practices as audience researchers in the performing arts and the practical factors that contribute to the dominance of the positive in audience evaluation.