Phase behavior and nanomechanical mapping of block ionomer complexes

Block ionomer complexes SSEBS-c-PCL were prepared, as a consequence of proton transfer from the sulfonic acid of sulfonated polystyrene-block- poly(ethylene-ran-butylene)-block-polystyrene (SSEBS) to the tertiary amine of a tertiary amine terminated poly(?-caprolactone) (APCL). The phase behavior of SSEBS-c-PCL was thoroughly investigated and the results showed that APCL in SSEBS-c-PCL displays unique crystallization behavior owing to the influence of interactions between the amine and sulfonic acid groups as well as the effects of confinement. Further, small-angle X-ray scattering study revealed that SSEBS-c-PCL displays a less ordered micro-phase structure compared to SSEBS. A quantitative mapping of mechanical properties at the nanoscale was achieved using peak force mode atomic force microscopy. It is found that the block ionomer complex possesses a higher average elastic modulus after complexation with crystallizable APCL. Additionally, the moduli for both hard and soft phases increase and the phase with higher modulus assignable to the hard SPS component shows much more pronounced changes after complexation, confirming that APCL interacts mainly with the SPS blocks. This provides an understanding of the composition and nanomechanical properties of these new block ionomer complexes and an alternative insight into the micro-phase structures of multi-phase materials.

Context matters: SETE mapping of teacher education in diverse contexts

The Studying the Effectiveness of Teacher Education (SETE) project (2010- ) investigates, as a priority, how well equipped teacher education graduates are to meet the requirements of diverse school settings. This paper outlines notions of diversity as reflected in large-scale longitudinal data sets including quantitative teacher and principal surveys (n=5,000+), national audit of teacher education programs (n=500+), and analysis of data from intensive case studies which track 170 graduate teachers through the first three years of their teaching career.

New opportunities for quantitative and time efficient 3D MRI of liquid and solid electrochemical cell components: Sectoral Fast Spin Echo and SPRITE.

The ability to image electrochemical processes in situ using nuclear magnetic resonance imaging (MRI) offers exciting possibilities for understanding and optimizing materials in batteries, fuel cells and supercapacitors. In these applications, however, the quality of the MRI measurement is inherently limited by the presence of conductive elements in the cell or device. To overcome related difficulties, optimal methodologies have to be employed. We show that time-efficient three dimensional (3D) imaging of liquid and solid lithium battery components can be performed by Sectoral Fast Spin Echo and Single Point Imaging with T1 Enhancement (SPRITE), respectively. The former method is based on the generalized phase encoding concept employed in clinical MRI, which we have adapted and optimized for materials science and electrochemistry applications. Hard radio frequency pulses, short echo spacing and centrically ordered sectoral phase encoding ensure accurate and time-efficient full volume imaging. Mapping of density, diffusivity and relaxation time constants in metal-containing liquid electrolytes is demonstrated. 1, 2 and 3D SPRITE approaches show strong potential for rapid high resolution (7)Li MRI of lithium electrode components.

Yaringa and French Island Marine National Park habitat mapping

Deakin University and the Department of Primary Industries were commissioned by ParksVictoria (PV) to create two updated habitat maps for Yaringa and French Island MarineNational Parks. The team obtained a ground-truth data set using in situ video and still photographs. This dataset was used to develop and assess predictive models of benthic marine habitat distributions incorporating data from World-View-2 imagery atmospherically corrected by CSIRO and LiDAR (Light Detection and Ranging) bathymetry. In addition, the team applied an unsupervised classification approach to an aerial photograph to assess the differences between the two remote sensors. This report describes the results of the mapping as well as the methodology used to produce these habitat maps.This study has provided mapping of intertidal and subtidal habitats of Yaringa and FrenchIsland MNPs at a 2 m resolution with fair to good accuracies (Kappa 0.40-0.75). These were combined with mangrove and saltmarsh habitats recently mapped by Boon et al. (2011) to provide compete-coverage habitat maps of Yaringa and French Island MNPs.The mapping showed that Yaringa MNP was dominated by mangroves, wet saltmarsh and dense Zostereaceae, covering 33%, 29% and 19%, respectively. Similarly, intertidalvegetation and subtidal vegetation (dominated by Zosteraceae) covered 26% and 25% ofFrench Island MNP. However, as a result of turbidity and missing satellite imagery 27% ofFrench Island MNP remains unmapped.The coupling of WV-2 and LiDAR reduced potential artefacts (e.g. sun glint causing whiteand black pixels known as the “salt and pepper effect”). The satellite classification appeared to provide better results than the aerial photography classification. However, since there is a two-year difference between the capture of the aerial photography and the collection of the ground-truth data this comparison is potentially temporally confounded. It must also be noted that there are differences in costs of the data,the spatial resolution between the two datasets (i.e. WV-2 = 2 m and the Aerial = 0.5 m) and the amount spectral information contained in the data (i.e. WV-2 = 8 bands and the aerial = 4 bands), which may ultimately determine its utility for a particular project.The spatial assessment using FRAGSTATs of habitat patches within Yaringa MNP provides a viable and cost effect way to assess habitat condition (i.e. shape, size and arrangement).This spatial assessment determined that dense Zosteraceae and NVSG habitat classeswere generally larger in patch size and continuity than the medium/sparse Zosteraceaehabitat. The application spatial techniques to time-series mapping may provide a way toremotely monitor the change in the spatial characteristics of marine habitats.This work was successful in providing new baseline habitat maps using a repeatable method meaning that any future changes in intertidal and shallow water marine habitats may be assessed in a consistent way with quantitative error assessments. In wider use, these maps should also allow improved conservation planning, fisheries and catchment management, and contribute toward infrastructure planning to limit impacts on Western Port.

Mutational interference mapping experiment (MIME) for studying RNA structure and function

RNA regulates many biological processes; however, identifying functional RNA sequences and structures is complex and time-consuming. We introduce a method, mutational interference mapping experiment (MIME), to identify, at single-nucleotide resolution, the primary sequence and secondary structures of an RNA molecule that are crucial for its function. MIME is based on random mutagenesis of the RNA target followed by functional selection and next-generation sequencing. Our analytical approach allows the recovery of quantitative binding parameters and permits the identification of base-pairing partners directly from the sequencing data. We used this method to map the binding site of the human immunodeficiency virus-1 (HIV-1) Pr55(Gag) protein on the viral genomic RNA in vitro, and showed that, by analyzing permitted base-pairing patterns, we could model RNA structure motifs that are crucial for protein binding.

The burden of neck pain: its meaning for persons with neck pain and healthcare providers, explored by concept mapping

PURPOSE: To empirically define the concept of burden of neck pain. The lack of a clear understanding of this construct from the perspective of persons with neck pain and care providers hampers adequate measurement of this burden. An additional aim was to compare the conceptual model obtained with the frequently used Neck Disability Index (NDI). METHODS: Concept mapping, combining qualitative (nominal group technique and group consensus) and quantitative research methods (cluster analysis and multidimensional scaling), was applied to groups of persons with neck pain (n = 3) and professionals treating persons with neck pain (n = 2). Group members generated statements, which were organized into concept maps. Group members achieved consensus about the number and description of domains and the researchers then generated an overall mind map covering the full breadth of the burden of neck pain. RESULTS: Concept mapping revealed 12 domains of burden of neck pain: impaired mobility neck, neck pain, fatigue/concentration, physical complaints, psychological aspects/consequences, activities of daily living, social participation, financial consequences, difficult to treat/difficult to diagnose, difference of opinion with care providers, incomprehension by social environment, and how person with neck pain deal with complaints. All ten items of the NDI could be linked to the mind map, but the NDI measures only part of the burden of neck pain. CONCLUSION: This study revealed the relevant domains for the burden of neck pain from the viewpoints of persons with neck pain and their care providers. These results can guide the identification of existing measurements instruments for each domain or the development of new ones to measure the burden of neck pain.