In-situ confocal Raman monitoring of evaporation process during protein crystallization

We have introduced an in-situ Raman monitoring technique to investigate the crystallization process inside protein drops. In addition to a conventional vapour-diffusion process, a novel procedure which actively stimulates the evaporation from a protein drop during crystallization was also evaluated, with lysozyme as a model protein. In contrast to the conventional vapour-diffusion condition, the evaporation-stimulated growth of crystals was initiated in a simple dehydration scheme and completed within a significantly shorter time. To gain an understanding of crystallization behaviours under the conditions with and without such evaporation stimulation, confocal Raman spectroscopy combined with linear regression analysis was used to monitor both lysozyme and HEPES buffer concentrations in real time. The confocal measurements having a high spatial resolution and good linear response revealed areas of local inhomogeneity in protein concentration when the crystallization started. The acquired concentration profiles indicated that (1)ÿthe evaporation-stimulated crystallization proceeded with protein concentrations lower than those under conventional vapour diffusion, and (2)ÿcrystals under the evaporation-stimulated condition were noticeable within an early stage of crystallization before the protein concentration approached its maximum value. The HEPES concentration profiles, on the other hand, increased steadily towards the end of the process regardless of the conditions used for crystallization. In particular, the observed local inhomogeneities specific to protein distribution suggested an accumulation mechanism of protein molecules that initiates the nucleation of crystals.

Re-discovering Wardell’s Chantry Chapel for Lord Petre : a crumbling fabulation

In January 2009 The Times reported that the Historic Chapels Trust (HCT) was undertaking the preservation and conservation of the Chantry Chapel of Thorndon Hall, near Brentwood, Essex, England, once the seat of the Petre family, one of England’s oldest Catholic families. The chapel has lain severely neglected for many years with missing and loose tiles, blocked gutters, and heavily eroded stonework. In spite of its desperate need of repair, inside, glimpses of the richly carved and lavishly decorated interior remain, witness to exquisite craftsmanship. Because of its quality Nikolas Pevsner had attributed the building to A W N Pugin. More recent research has established that in fact William Wardell was the architect.

By 1854, when Lord Petre commissioned this mausoleum for his estate, Wardell would have been especially known for his London curvilinear decorated churches at Greenwich, Clapham and Hammersmith. Wardell produced three complete sets of drawings for the Chantry Chapel. Drawings for all three designs are extant, and give valuable insights into Wardell's design methods and the evolution of his design thinking. They raise questions about Early Victorian and High Victorian Gothic sensibilities and establish Wardell’s architectural and design credentials beyond a doubt. This paper explores Wardell’s debt to Pugin, posits the Chantry Chapel as a rival to Pugin’s St Giles Church, Cheadle, and considers the question of patronage.

Now acknowledged to be ‘of outstanding architectural and historic interest ‘ by HCT and English Heritage, the Chantry chapel - a crumbling fabulation - is the subject of major heritage considerations. Questions about authenticity in rebuilding and reconstruction are currently overridden by the urgent need to secure the structure from collapse.

The establishment of the Judicial Appointments Commission in Malaysia to improve the constitutional method of appointing the judges of the superior courts : a critical study

The Judicial Appointments Commission was established in Malaysia in 2009 to ensure unbiased selection of judicial candidates for the consideration of the Prime Minister, who has the final say regarding the appointment of judges to the superior courts. But the provisions concerning Prime Minister’s power to appoint the majority of the members of the Commission and his unfettered power of removing four of the five appointed members without assigning any reason, have calculatedly been devised for ensuring the selection of judicial candidates having right political patronage in accordance with the covert wishes of the Prime Minister. Furthermore, the Prime Minister’s power of rejecting the Commission’s recommendations of multiple candidates renders the undertaking of a lengthy process of selection unproductive and useless. Thus the Judicial Appointments Commission has become a superfluous body with an ineffective modus operandi to attain the stipulated objectives of improving and complementing the constitutional method of appointing judges to the superior courts. Since the Federal Constitution of Malaysia has not empowered the Parliament to enact a law providing for the establishment of a Judicial Appointments Commission, it also appears that the Judicial Appointments Commission Act 2009 is an invalid piece of legislation.

Political connection and leverage: Some Malaysian evidence

This paper extends prior work on the linkage between politically connected (PCON) firms and capital structure in developing countries. Specifically, this paper focuses on the association between Malaysian PCON firms and leverage, and is motivated by the results of Fraser et al. (2006) who report a positive association between leverage and political patronage. Controlling for a potential misspecification in that paper, this study documents that a significant proportion (almost 12%) of the Malaysian PCON firms have negative equity, and builds on the previous paper by providing fresh evidence that market to book ratio is positively associated with leverage, and that borrowing PCON firms have significantly lower ROA compared to non-PCON firms. © 2012.

An investigation of neighborhood-level social, economic and physical factors for railway suicide in Victoria, Australia

BACKGROUND: This study investigates the associations between railway suicide and neighborhood social, economic, and physical determinants using postcode-level data. It also examines whether the associations are influenced by having high concentration of high-risk individuals in a neighborhood area. METHODS: Railway suicide cases from Victoria, Australia for the period of 2001-2012, their age, sex, year of death, usual residential address and suicide location were obtained from the National Coronial Information System. Univariate negative binomial regression models were used to estimate the association between railway suicide and neighborhood-level social, economic and physical factors. Variables which were significant in these univariate models were then assessed in a multivariate model, controlling for age and sex of the deceased and other known confounders. RESULTS: Findings from the multivariate analysis indicate that an elevated rate of railway suicide was strongly associated with neighborhood exposure of higher number of railway stations (IRR=1.30 95% CI=1.16-1.46). Other significant neighborhood risk factors included patronage volume (IRR=1.06, 95% CI=1.02-1.11) and train frequency (IRR=1.02, 95% CI=1.01-1.04). An increased number of video surveillance systems at railway stations and carparks was significantly associated with a modest reduction in railway suicide risk (IRR=0.93, 95% CI=0.88-0.98). These associations were independent of concentration of high-risk individuals. LIMITATIONS: Railway suicide may be under-reported in Australia. CONCLUSIONS: Interventions to prevent railway suicide should target vulnerable individuals residing in areas characterized by high station density, patronage volume and train frequency.

Sarah Bernhardt

Sarah Bernhardt is the most famous actress of the late nineteenth century stage. Celebrated by an emerging and very vocal group of young female workers and artisans in her native Paris in the late 1860s and the 1870s called "les saradoteurs she went on to become the most popular actress of her generation in Europe, North America, and Australia. Attention has been paid to her "golden voice," the clever ways she marketed and promoted herself, her pioneering patronage of artists such as Alphonse Mucha and René Lalique and her capacity to be at once a successful actress, manager, and theatre director. Scant attention has been paid, however, to Bernhardt’s involvement and success in the early motion picture film industry, both in France and abroad.

Sarah Bernhardt

Sarah Bernhardt is the most famous actress of the late nineteenth century stage. Celebrated by an emerging and very vocal group of young female workers and artisans in her native Paris in the late 1860s and the 1870s called "les saradoteurs she went on to become the most popular actress of her generation in Europe, North America, and Australia. Attention has been paid to her "golden voice," the clever ways she marketed and promoted herself, her pioneering patronage of artists such as Alphonse Mucha and René Lalique and her capacity to be at once a successful actress, manager, and theatre director. Scant attention has been paid, however, to Bernhardt’s involvement and success in the early motion picture film industry, both in France and abroad.

Celebrating transgressive celebrity : Sarah Bernhardt

Sarah Bernhardt (born Sara-Marie-Henriette Bernard, 1844-1923) was the most famous actress of the late nineteenth century. Celebrated for her golden voice, for her use of the spiral as the building block for movement on the theatrical stage, for an innovative use of costume and jewellery, for her patronage emerging artists, and for the business acumen that saw her fast become a household name across the globe, she was the progenitor of celebrity as we know it today. This article explores the ways in which her achievements can be considered 'dangerous' today.