Effects of human activities on rocky shores of the Surf Coast Shire: scientific basis for management at the regional level

The Surf Coast Shire in regional Victoria contains some of the most spectacular coastline in Australia, running from Point Impossible in the east to just west of the resort town of Lorne. The Surf Coast Shire council is committed to ecologically sustainable tourism based on its coastal assets, including the important intertidal environments. The challenge for the Shire is to protect and enhance the biodiversity of its intertidal areas whilst allowing for their sustainable use as a critical component of the local economy. In order to do this the Council needed to identify the conservation values of intertidal areas within the shire and assess the impacts that current human use has on these values. The impacts of shellfish collecting on rocky shores were identified as an issue of particular concern. We have conducted a research project with the Shire to provide a scientific basis for management decisions. The principal aims of this project were to: (1) determine the patterns of human use of intertidal habitats; (2) measure the impacts of human usage on biological communities and species populations; and (3) to identify intertidal sites of regional conservation significance for the Surf Coast Shire. Surveys of human usage identified reef walking, looking in rock pools and fossicking as major uses of rocky shores within the Surf Coast. This poster reports the effects of this usage on gastropod populations of rocky shores within the Surf Coast Shire. A small proportion of visitors collected intertidal organisms. Shores were categorized as high or low use based on total numbers of people observed at each shore over the first year of the project. Mean size and catch per unit effort were compared for several gastropod species between high use and low use shores. The results presented here show that the populations of some gastropod species are of smaller mean size and less abundant on high use shores than on low use shores. There was also a noticeable difference in degree of effect detected between sandstone and mudstone shores. The implications of these results are briefly discussed in terms of management options available to the Shire.

Inverse opal nanoassemblies : novel architectures for gas sensors the SnO2:Zn case

A novel technique is here presented, based on inverse opal metal oxide structures for the production of high quality macro and meso-porous structures for gas sensing. Taking advantage of a sol-gel templated approach. different mixed semiconducting oxides with high surface area, commonly used in chemical sensing application, were synthesized. In this work we report the
comparison between SnO₂ and SnO₂:Zn. As witnessed by Scanning and Transmission Electron Microscopy (SEM and TEM) analyses and by Powder x-ray Diffraction (PX RD), highly ordered meso-porous structures were formed with oxide crystalline size never exceeding 20 nm . The filled templates. in form of thick films, were bound to allumina substrate with Pt interdigitated contacts
and Pt heater, through in situ calcination, in order to perform standard electrical characterization. Pollutant gases like CO and NO₂ and methanol. as interfering gas, were used for the targeted electrical gas tests. All samples showed low detection limits towards both reducing and oxidizing species in low temperature measurements. Moreover, the addition of high molar percentages of Zn( II) affected the beha viour of electrical response improv ing the se lecti vity of the proposed system.

Ionic liquid-assisted synthesis of polyaniline/gold nanocomposite and its biocatalytic application

In this report, a novel chemical synthesis of polyaniline/gold nanocomposite is explored using ionic liquid (IL) 1-Butyl-3-methylimidazolium hexafluorophosphate. The direct chemical synthesis of polyaniline/gold nanocomposite was initiated via the spontaneous oxidation of aniline by AuCl4 − in IL. A nearly uniform dispersion of polyaniline/Au particles with a diameter of 450 ± 80 nm was produced by this method, which indicates that this method is more suitable for controlling particle dimensions. It was also found that the electrical conductivity of the polyaniline/gold nanocomposite was more than 100 times higher than that of the pure polyaniline nanoparticles. The polyaniline/gold nanocomposite displays superior function in the biocatalytic activation of microperoxidase-11 because of the high surface area of the assembly and the enhanced charge transport properties of the composite material. We also report the possible application of polyaniline/gold nanocomposite as a H2O2 biosensor.

Three dimensional scaffolds from multilobal fibres for enhanced cell growth

Multilobal fibres are irregularly shaped fibres with several surface channels or grooves. Scaffolds created from these fibres have high surface area which may enhance cell density. This study compared the cell growth of dermal fibroblasts and osteoblast-like SaOS2 cells on multilobal fibre nonwoven scaffolds to the conventional fibre scaffolds. Cells were cultured on round nylon, trilobal nylon, round polyethylene terephthalate (PET) and multilobal PET scaffolds for 14 days. There were more cells growing on trilobal nylon and PET multilobal scaffolds than their round counterparts. The preference to the type of multilobal scaffolds was cell dependent. The density of fibroblast increased 37% on trilobal nylon compared to round nylon scaffolds after 14 days of culture. SaOS2 cells preferred the multilobal PET scaffolds, exhibiting a 66% increase in cell number after 14 days of culture. Scaffolds manufactured from multilobal fibres have the ability to accommodate a high number of cells, demonstrating a great potential in tissue engineering applications.

Recent trends in nanomaterials immobilised enzymes for biofuel production

Application of nanomaterials as novel supporting materials for enzyme immobilisation has generated incredible interest in the biotechnology community. These robust nanostructured forms, such as nanoparticles, nanofibres, nanotubes, nanoporous, nanosheets, and nanocomposites, possess a high surface area to volume ratios that can cause a high enzyme loading and facilitate reaction kinetics, thus improving biocatalytic efficiency for industrial applications. In this article, we discuss research opportunities of nanoscale materials in enzyme biotechnology and highlight recent developments in biofuel production using advanced material supports for enzyme immobilisation and stabilisation. Synthesis and functionalisation of nanomaterial forms using different methods are highlighted. Various simple and effective strategies designed to result in a stable, as well as functional protein-nanomaterial conjugates are also discussed. Analytical techniques confirming enzyme loading on nanomaterials and assessing post-immobilisation changes are discussed. The current status of versatile nanomaterial support for biofuel production employing cellulases and lipases is described in details. This report concludes with a discussion on the likely outcome that nanomaterials will become an integral part of sustainable bioenergy production.

Template-free synthesis of functional 3D BN architecture for removal of dyes from water.

Three-dimensional (3D) architectures are of interest in applications in electronics, catalysis devices, sensors and adsorption materials. However, it is still a challenge to fabricate 3D BN architectures by a simple method. Here, we report the direct synthesis of 3D BN architectures by a simple thermal treatment process. A 3D BN architecture consists of an interconnected flexible network of nanosheets. The typical nitrogen adsorption/desorption results demonstrate that the specific surface area for the as-prepared samples is up to 1156 m(2) g(-1), and the total pore volume is about 1.17 cm(3) g(-1). The 3D BN architecture displays very high adsorption rates and large capacities for organic dyes in water without any other additives due to its low densities, high resistance to oxidation, good chemical inertness and high surface area. Importantly, 88% of the starting adsorption capacity is maintained after 15 cycles. These results indicate that the 3D BN architecture is potential environmental materials for water purification and treatment.

Highly crumpled boron nitride nanosheets as adsorbents: scalable solvent-less production

Boron nitride nanosheets (BNNSs), so-called “white graphene”, have recently received increasing attention, both theoretically and experimentally. Although many synthetic procedures have been proposed for the synthesis of BNNSs, finding a simple, solvent-less, catalyst-free, and large-scale production route is still a challenge. Here, a facile, solvent-less, low cost, and high yield process is developed, in which mechanical solid-state exfoliation allows scalable production of crumple BNNSs from commercial BN powders with a high surface area. Importantly, these BNNSs show unprecedentedly high adsorption of proteins described by various adsorption isotherms and kinetics models. In addition, the saturated BNNSs exhibit excellent recyclability, and maintain a high sorption capacity even after five cycles through simply regeneration process of heating in air. This easy recyclability route further demonstrates the great potential of BNNSs for water cleaning application.

Drug loading and release studies for milled silk particles of different sizes

Milled silk particles with volume median particle size (d(0.5)) of 7 μm and 281 nm as well as silk snippets were used for loading of model drugs Orange G, Azophloxine, Rhodamine B, and Crystal Violet. Loading and release of these chemicals depended on the size of silk particles, pH, and the structure and properties of model drugs. Both types of silk particles reached equilibrium loading in less than 10 min due to high surface area whereas silk fibres needed more than 2-3 days to reach equilibrium, depending on the drug type. The uptake rate in fibres could be improved by increasing temperature. Both fibres and particles could slowly release the drugs over many days at 37 °C without a significant initial burst. As particle size decreased, the amount of model drug release also decreased. The release of drugs by the silk fibres was quicker than the silk particles.

Advanced N-doped mesoporous molybdenum disulfide nanosheets and the enhanced lithium-ion storage performance

With the increasing interest in two-dimensional van der Waals materials, molybdenum disulfide (MoS2) has emerged as a promising material for electronic and energy storage devices. It suffers from poor cycling stability and low rate capability when used as an anode in lithium ion batteries. Here, N-doped MoS2 nanosheets with 2-8 atomic layers, increased interlayer distance, mesoporous structure and high surface area synthesised by a simple sol-gel method show an enhanced lithium storage performance, delivering a high reversible capacity (998.0 mA h g-1, 50 mA g-1), high rate performance (610 mA h g-1, 2 A g-1), and excellent cycling stability. The excellent lithium storage performance of the MoS2 nanosheets might be due to the better electrical and ionic conductivity and improved lithium ion diffusion which are related to their structural characteristics and high concentration N doping. The possible mechanism of the improved performance is proposed and discussed.

Superhydrophobic and superoleophilic micro-wrinkled reduced graphene oxide as a highly portable and recyclable oil sorbent.

The potential of superhydrophobic and superoleophilic microwrinkled reduced graphene oxide (MWrGO) structures is here demonstrated for oil spill cleanup. The impact of the thickness of MWrGO films on the sorption performance of three different oils was investigated. Water contact angles across the MWrGO surfaces were found to exceed 150°, while oil could be easily absorbed by the microwrinkled structures of MWrGO within seconds after contact. Although the oil surface diffusion rate was not found to be dependent on the thickness of the graphene oxide films, the oil sorption capacity was the largest with the thinner MWrGO films due to the high surface area resulting from their fine surface texture. Furthermore, the composite films can be repeatedly used for at least 20 oil sorption-removal cycles without any notable loss in selectivity and uptake capacity. These MWrGO/elastomer composite films could be applied as a potential candidate material for future oil spill cleanup.

Enhancement of ion dynamics in organic ionic plastic crystal/PVDF composite electrolytes prepared by co-electrospinning

Electrospun fibers are widely used in composite material design and fabrication due to their high aspect ratio, high surface area and favorable mechanical properties. In this report, novel organic ionic plastic crystal (OIPC) modified poly(vinylidene difluoride) (PVDF) composite fiber membranes were prepared by electrospinning. These composite materials are of interest for application as solid electrolytes in devices including lithium and sodium batteries. The influence of the OIPC, N-ethyl-N-methylpyrrolidinium tetrafluoroborate [C2mpyr][BF4], on the morphology and phase behavior of the composite fibers was investigated by scanning electron microscopy and Fourier transform infrared spectroscopy. Compared with pure electrospun PVDF fibers, which have an electroactive β phase and a small amount of non-polar α phase, the ion-dipole interaction between OIPC and the polymer in the co-electrospun composite system can reduce the non-polar α phase PVDF, resulting in almost entirely electroactive β phase PVDF. Differential scanning calorimetry shows that the ion-dipole interaction between the OIPC and PVDF can also interrupt the crystalline structure of the OIPC. Solid state NMR analysis also reveals different molecular dynamics of the [C2mpyr][BF4] in co-electrospun fibers compared with pure OIPC. Thus, electrospun [C2mpyr][BF4]/PVDF composite fibers that combine both increased ionic conductivity and almost pure β phase PVDF are demonstrated.

Home-range use by the brush-tailed phascogale (phascogale tapoatafa) (marsupialia) in High-quality, spatially limited habitat

Nine phascogales (7 females, 2 males) were radio-tracked between March and July 1999 to investigate the spatial organisation of this species in spatially limited habitat near Euroa, Victoria. In this area, approximately 3.6% of the original woodland vegetation remains after 150 years of agricultural clearing. Most wooded habitat is confined to narrow linear strips along roads and streams. However, these remnants are on fertile soils and, because they have not experienced intensive harvesting, the density of large old trees is over 10 times that found in nearby State Forests and Parks. Female phascogales were monitored for 13–38 days over periods of 5–15 weeks. The size of home ranges of females was 2.3–8.0 ha, and averaged 5.0 ha. This value is one-eighth the mean home-range size previously recorded for the species in contiguous forest in Victoria. All individuals used multiple nest trees, with nests generally located in trees >80 cm diameter at breast height. Although fragmented and spatially limited, the stands of large old trees on productive soils near Euroa provide a network of well connected, high-quality habitat for phascogales. The relatively dense population of phascogales in these remnants suggests that prior to agricultural clearing and timber harvesting, phascogales may have been much more common in Victoria than at present.

Use of high resolution digital multi-spectral imagery to assess the distribution of disease caused by Phytophthora cinnamomi on heathland at Anglesea, Victoria

Disease caused by the soilborne plant pathogen Phytophthora cinnamomi causes long-term floristic and structural changes in native vegetation communities in Australia. Key components of the management of this disease are to know where it occurs and the rate at which it spreads. The distribution of P. cinnamomi has generally been assessed as locality points of infestation and mapping the extent of diseased vegetation in any area is difficult and costly. This study was undertaken in P. cinnamomi-infested heathland communities in southern Victoria, Australia, where the symptoms of P. cinnamomi arise as a mosaic within healthy vegetation. We investigated the potential to improve the efficiency and effectiveness of mapping and monitoring vegetation affected by P. cinnamomi using digital multi-spectral imaging. This technique was developed for the purposes of monitoring vegetation and provides a single, seamless ortho-rectified digital image over the total area of interest. It is used to spatially quantify small differences in the characteristics of vegetation. In this study, the symptoms of disease caused by P. cinnamomi infestation were related to differences in the imagery and were used to map areas of infestation. Comparison of the digital multi-spectral imaging indications with on-ground observations gave moderate accuracy between the datasets (κ = 0.49) for disease and healthy indications. This study demonstrates the ability of the technique to determine disease extent over broad areas in native vegetation and provides a non-invasive, cost effective tool for management.

Perceived neighborhood environment and park use as mediators of the effect of area socio-economic status on walking behaviors

Background: Access to local parks can affect walking levels. Neighborhood environment and park use may influence relationships between neighborhood socioeconomic status (SES) and walking.

Methods: Self-report data on perceived park features, neighborhood environment, park use, neighborhood walking and sociodemographics
were obtained from a sample of Australian adults, living in high/low SES areas. Surveys were mailed to 250 randomly selected households within 500m of 12 matched parks. Mediating effects of perceived environment attributes and park use on relationships between area-SES and walking were examined.

Results: Mean frequency of local park use was higher for high-SES residents (4.36 vs 3.16 times/wk, P < .01), who also reported higher levels of park safety, maintenance, attractiveness, opportunities for socialization, and neighborhood crime safety, aesthetics, and traffic safety. Safety and opportunity for socialization were independently positively related to monthly frequency of visits to a local park which, in turn, was positively associated with walking for recreation and total walking. Residents of higher SES areas reported an average 22% (95% CI: 5%, 37%) more weekly minutes of recreational walking than their low SES counterparts.

Conclusion: Residents of high-SES areas live in environments that promote park use, which positively contributes to their weekly amounts of overall and recreational walking.

Recreational use of a marine protected area: Point Lonsdale, Victoria

This This article reports findings from observational and questionnaire surveys of visitors to a Marine Protected Area (MPA) at Point Lonsdale, Victoria. The MPA was established primarily to protect the biodiversity of intertidal rock platforms, with only limited restrictions being placed on fishing. Visitor surveys were undertaken to identify and quantify recreational uses, assess level of compliance with regulations, identify the uses most likely to have damaging impacts on biota, and to assess awareness of, and support for, this MPA and for MPAs in general. A questionnaire survey of visitors supported observational survey findings concerning recreational use patterns and provided information on awareness about and attitudes towards conservation measures for this location and for Victoria's marine environment. The finding that about half the visitors were not aware that
they were visiting a marine protected area has implications for future management of this area and MPAs in general. Most visitors indicated support for the concept of marine conservation areas and marine protected areas.