29 resultados para Subtractive cDNA libraries


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Academic libraries are taken to refer here to two particular types of library: university libraries and those libraries which serve the vocational education and training (VET) sector through colleges or institutes of technical and further education (TAFE). (School libraries are dealt with in a separate chapter.) Universities cover undergraduate programs, principally Bachelors degrees, and postgraduate programs such as the Graduate Certificate, Graduate Diploma, Masters degrees and doctoral programs. The main TAFE awards are Certificate, Diploma and Advanced Diploma. Universities are largely funded by national government - the federal Commonwealth Government in Australia's case - although, as elsewhere, an increasing amount of university funding needs to come from non-government sources, particularly research funding. In Australia institutes of TAFE are funded by state and territory governments, although from 2005 the Federal Government began providing funding for the development of technical colleges outside the TAFE sector that would provide vocational education for secondary school age students. This latter development may well be affected by the change in federal government in late 2007.

The mission for academic libraries globally is to support the teaching, learning and (where appropriate) research activities of their parent institutions. In Australia and New Zealand, universities and their libraries have also had a long tradition of reaching out to the community, contributing to the cultural and intellectual life of the nation. Australia has thirty-nine universities; of which thirty-seven are public institutions and two are private. New Zealand has eight universities. The libraries supporting these institutions are diverse, of high quality and innovative. Based on 2005 figures, there are sixty-eight institutions in Australia's VET sector, with over 1,100 campuses, 1.7 million students and some eleven per cent of Australia's working age population accessing TAPE (Oakley & Vaugha 2007: 43).

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In Australia and New Zealand, the term 'special library' refers to libraries that provide resources and services to employees of an organization or industry.  The majority have collections and/or services supporting a specific subject area.  These include, but are not limited to, libraries in government department, law firms, private companies, banking and finance institutions, research organizations, religious groups and professional associations.

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Is the industry in chaos? How do we to meet the future information needs of the international scholarly community?

The one certainty is that forecasting is never easy. Libraries, vendors and publishers are all working in a rapidly changing environment. It is a fair prediction that there will be fewer participants and less competition in the marketplace. The potential for libraries to be locked into one vendor for access to electronic journals is real. Whether this access will be in perpetuity, no one can give an absolute guarantee. Intellectual property rights, commercial viability and communication standards are all of concern.

We've seen the vision, what's happening now? The Australian academic and research library market has an international reputation for being informed, frank and through necessity, pragmatic. When planning information access and delivery for the next two to five years we are told libraries need a reasonable indication of what is real. Vendors, more than ever, are contributing to a shared understanding amongst libraries, publishers and vendors of the priorities and concerns of different sectors of the industry.

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We have identified a major allergenic protein from rye-grass pollen, tentatively designated Lol pIb of 31kDa and with pI 9.0. A cDNA clone encoding Lol pIb has been isolated, sequenced, and characterized. Lol pIb is located mainly in the starch granules. This is a distinct allergen from Lol pI, which is located in the cytosol. Lol pIb is synthesized in pollen as a pre-allergen with a transit peptide targeting the allergen to amyloplasts. Epitope mapping of the fusion protein localized the IgE binding determinant in the C-terminal domain.

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The bias of A-rich codons in HIV-1 pol is thought to be a record of hypermutations in viral genomes that lack biological functions. Bioinformatic analysis predicted that A-rich sequences are generally associated with minimal local RNA structures. Using codon modifications to reduce the amount of A-rich sequences within HIV-1 genomes, we have reduced the flexibility of RNA sequences in pol to analyze the functional significance of these A-rich ‘structurally poor’ RNA elements in HIV-1 pol. Our data showed that codon modification of HIV-1 sequences led to a suppression of virus infectivity by 5–100-fold, and this defect does not correlate with, viral entry, viral protein expression levels, viral protein profiles or virion packaging of genomic RNA. Codon modification of HIV-1 pol correlated with an enhanced dimer stability of the viral RNA genome, which was associated with a reduction of viral cDNA synthesis both during HIV-1 infection and in a cell free reverse transcription assay. Our data provided direct evidence that the HIV-1 A-rich pol sequence is not merely an evolutionary artifact of enzyme-induced hypermutations, and that HIV-1 has adapted to rely on A-rich RNA sequences to support the synthesis of viral cDNA during reverse transcription, highlighting the utility of using ‘structurally poor’ RNA domains in regulating biological process.

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The article discusses the Australian government's Higher Education Participation and Partnerships Program. The program aims to encourage greater participation of students from low socioeconomic backgrounds in higher education. The article identifies potential means by which a library may contribute to student retention and outlines one library's approach to investigating its contribution.

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Climate change adaptation and mitigation continues to be a prevalent discourse in this country and internationally in both the sciences and the arts. While various types and degrees of change are evident, the quantification of these changes including their scope and diversity have challenged conventional sciences. This is demonstrated in their inability to succinctly answer key questions about change including the degree of change and associated patterns and consequences. Most of this discourse is nested in a temporal band comprising the last 100-200 years of data and evidence, and very much informed by Western science perspectives and protocols. Little attempt has been made to engage with Australian Indigenous communities whom possess environmental knowledge of some 10,000-100,000 years albeit embedded in their artistic and oral narrative 'histories'. This paper explores the role and values that Australian Aboriginals, the Indigenous peoples of the Australian content, can offer in shedding new light on this discourse While focusing upon a cross-peri-urban Indigenous investigation, it examines this discourse though the lens of their words, terms, sentences as a vehicle to better understand a longitudinal perspective about climate change adaptation pertinent to Australia.

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Recent studies have determined that many Android applications in both official and non-official online markets expose details of the users' smartphones without user consent. In this paper, we explain why such applications leak, how they leak and where the data is leaked to. In order to achieve this, we combine static and dynamic analysis to examine Java classes and application behaviour for a set of popular, clean applications from the Finance and Games categories. We observed that all the applications in our data set which leaked information (10%) had third-party advertising libraries embedded in their respective Java packages.

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This paper identifies the design qualities of library spaces that matter the most foruniversity students. Drawing upon the data from an online survey made available to students from the University of Queensland, Australia, a number of design-related considerations are examined including: acoustics, furniture, interior architecture, lighting, and thermal comfort. 1505 students completed the survey, which aimed to assess how effective and responsive library spaces are in meeting students’ needs and supporting their learning experiences. The survey included ‘Likert scale questions’ requiring students to rate their levels of satisfaction with different aspects of library spaces and ‘open-ended questions’ asking students to elucidate their ratings. Findings revealed that the qualities of physical spaces were ranked as the third mostsignificant category of reasons accounting for students’ preference for certain library buildings over others, and for their frequency of visit (behind “location” of the library building and then “access to books and course-related materials or resources”). Design-related themes which emerged from qualitative analysis highlighted students’ awareness of the impacts that the design of spaces and furniture can have on their learning experiences. The study concludes with recommendations informed by students’ expectations, needs and preferences in relation to the qualities and features of library spaces.