Spermatogenesis in the blue swimming crab, Portunus pelagicus, and evidence for histones in mature sperm nuclei

Spermatogenesis in the blue swimming crab, Portunus pelagicus, is described by light and electron microscopy. The testis is composed of anterior (AT) and posterior (PT) lobes, that are partitioned into lobules by connective tissue trabecula, and further divided into zones (germinal, transformation and evacuation), each with various stages of cellular differentiation. The vas deferens is classified into three distinct regions: anterior (AVD), median (MVD), and posterior (PVD), on the presence of spermatophores and two secretions, termed substance I and II. Based on the degree and patterns of heterochromatin, spermatogenesis is classified into 13 stages: two spermatogonia (SgA and SgB), six primary spermatocytes (leptotene, zygotene, pachytene, diplotene, diakinesis, and metaphase), a secondary spermatocyte (SSc), three spermatids (St 1–3), and a mature spermatozoon. Spermatid stages are differentiated by chromatin decondensation and the formation of an acrosomal complex, which is unique to brachyurans. Mature spermatozoa are aflagellated, and have a nuclear projection and a spherical acrosome. AUT-PAGE and Western blots show that, during chromatin decondensation, there is a reduction of most histones, with only small amounts of H2B and H3 remaining in mature spermatozoa.

Relationship of weaning weight to the mature liveweight of cashmere does on Australian farms

Median weaning weight and its relationship with the median mature liveweight of does was quantified for four commercial Australian cashmere farms in various parts of Australia. Individual liveweights, of does of all ages at the time of weaning in December, ranged from 9 to 61 kg. Individual farm means of adult does (>1 year old) ranged from 24.6 to 38.8 kg. The model for the logarithm of liveweight was: log₁₀(liveweight) = a + br ^{(Age + 0.6);} where a, b and r are parameters that are different for each farm. The result that the r parameter differs with farm was statistically significant (P = 9.4 10^-6). The percentage variance accounted for was 84.4% and the residual standard deviation was 0.042. Farms differed greatly in the median mature liveweight with some farms reaching ~44 kg and others only 31 kg. Median weaning weight was 14.1 kg (range 11.4-16.8 kg). Median weaning weight as a percentage of median mature doe liveweight on a particular farm varied from 32 to 42%. These weaning weights appear low in absolute and relative terms and thus are likely to incur production penalties.

Isolation of a gene preferentially expressed in mature anthers of rice (Oryza sativa L.)

Using monoclonal antibodies raised against pollen-specific proteins, we have isolated a cDNA clone, designatedOry-Cl from a rice anther cDNA expression library. A transcript corresponding to theOry-Cl gene showed preferential expression in anthers. This transcript was not detected in any vegetative tissues analysed. RNA gel blot analysis of different developmental stages of anthers showed that theOry-Cl gene is expressed at later stages of pollen development. In situ hybridisation showed that theOry-Cl transcript is only present in mature pollen.

Alternative pathways to traditional destinations : higher education for disadvantaged Australians

In 1985, the Higher Education Equity Program was introduced by the Australian Government to improve the participation of those persons from social groups traditionally under-represented within higher education. In 1990, the program was incorporated within A Fair Chance For All which provided more specific details of the government's desire for a system-wide approach to equity issues. One result has been the proliferation of access and equity programs conducted by universities around the country and aimed at redressing the disadvantage of potential students. The alleged success of these programs is based on greater participation in and graduation from Australian universities by individuals from targeted disadvantaged groups. The research reported here, however, would suggest that such programs are prone to co-opt the language of equity and social justice, dependent as they are on satisfying statistically-orientated program performance indicators in order to receive recurrent government funding. Further, the paper argues that success in achieving equity within Australian higher education will remain limited unless the structural arrangements that work to construct social inequalities in mainstream higher education are addressed.

Seeking romance and a once in a life-time experience : considering attributes that attract honeymooners to destinations

Couples on their honeymoon represent a vital part of the tourism market. Since the destination decision for honeymooners is a joint decision, paired in-depth interviews were conducted with ten British couples to explore their honeymoon destination decision making. The themes that arose from this qualitative study were consistent with more general studies on destination choice, including: culture, natural environment, and accommodation. New themes developed from the research included familiarity, romance, and budget. The results indicate that couples were more likely to stretch their budget for a honeymoon, or appear to be less concerned about the cost of their trip, as many see it as a once in a lifetime experience. This indicates that this valuable segment ofthe market should be further explored.

The conformation of the mature dimeric human immunodeficiency virus type 1 RNA genome requires packaging of pol protein

The packaging of a mature dimeric RNA genome is an essential step in human immunodeficiency virus type 1 (HIV-1) replication. We have previously shown that overexpression of a protease (PR)-inactive HIV-1 Gag-Pro-Pol precursor protein generates noninfectious virions that contain mainly monomeric RNA (M. Shehu-Xhilaga, S. M. Crowe, and J. Mak, J. Virol. 75:1834-1841, 2001). To further define the contribution of HIV-1 Gag and Gag-Pro-Pol to RNA maturation, we analyzed virion RNA dimers derived from Gag particles in the absence of Gag-Pro-Pol. Compared to wild-type (WT) dimeric RNAs, these RNA dimers have altered mobility and low stability under electrophoresis conditions, suggesting that the HIV-1 Gag precursor protein alone is not sufficient to stabilize the dimeric virion RNA structure. The inclusion of an active viral PR, without reverse transcriptase (RT) and integrase (IN), rescued the stability of the virion RNA dimers in the Gag particles but did not restore the mobility of the RNAs, suggesting that RT and IN are also required for virion RNA dimer maturation. Thin-section electron microscopy showed that viral particles deficient in RT and IN contain empty cone-shaped cores. The abnormal core structure indicates a requirement for Gag-Pro-Pol packaging during core maturation. Supplementing viral particles with either RT or IN via Vpr-RT or Vpr-IN alone did not correct the conformation of the dimer RNAs, whereas expression of both RT and IN in trans as a Vpr-RT-IN fusion restored RNA dimer conformation to that of the WT virus and also restored the electron-dense, cone-shaped virion core characteristic of WT virus. Our data suggest a role for RT-IN in RNA dimer conformation and the formation of the electron-dense viral core.

Mature reverse transcriptase (p66/p51) is responsible for low levels of viral DNA found in human immunodeficiency virus type 1 (HIV-1)

Reverse transcription of the HIV RNA genome is thought to occur in the host cell cytoplasm after viral adsorption. However, viral DNA has been isolated in cell-free virus particles. We have quantitated by polymerase chain reaction (PCR) amplification the amount of viral DNA in virions as compared to RNA. Virus produced by proviral DNA transfections of cos-7 cells or by chronically-infected H9 cells; neither of which express the cell surface CD4 receptor, contained at least 1000 times more viral RNA than DNA. In contrast, only 60 times more RNA than DNA was present in virus particles produced by transfection of Jurkat cells, which were CD4-positive and thus potentially susceptible to superinfection. Protease-defective virus, carrying only the precursor of reverse transcriptase (RT) p160gag-pol, contained virtually no detectable DNA. These results indicate that only mature RT (p66/p51) and not its precursor (p160gag-pol) is responsible for the presence of viral DNA in HIV.

Walkable area within which destinations matter : differences between Australian and Japanese cities

Adults’ walking for transport is important for health benefits and can be associated with availability of destinations such as shops and services within a walking distance of 10 to 15 minutes from home. However, relevant evidence is mostly from Western countries. This study examined associations of destinations with walking for transport in Australian and Japanese cities. Data were collected from Adelaide, Australia (n = 2508), and 4 Japanese cities (n = 1285). Logistic regressions examined associations of self-reported walking for transport with the number of destination types within walk-distance categories. Walking was significantly associated with the number of destination types within a 10-minute walk from home for Australia and with the number of destination types within a 6- to 20-minute walk for Japan. Further research is needed on why walking by residents of Japanese cities can be influenced by more distant local destinations than in Australia to inform physical activity–related environmental and policy initiatives.

Life in the really slow lane : loggerhead sea turtles mature late relative to other reptiles

1. Age at maturity is hard to estimate for species that cannot be directly marked or observed throughout their lives and yet is a key demographic parameter that is needed to assess the conservation status of endangered species. 2. For loggerhead turtles (Caretta caretta) in the North Atlantic and North Pacific, juvenile growth rates (c. 10 cm year−1) were calculated by examining size increases during transoceanic journeys; durations of which were estimated from satellite-tracked Lagrangian surface drifter buoy trajectories. 3. Lagrangian-derived growth estimates were used in a weighted loglinear model of size-specific growth rates for loggerhead turtles and combined with newly available information on size at maturity to estimate an age at maturity of 45 years (older than past estimates). 4. By examining the age at maturity for 79 reptile species, we show that loggerhead turtles, along with other large-bodied Testudine (turtle and tortoise) species, take longer to reach maturity than other reptile species of comparable sizes. This finding heightens concern over the future sustainability of turtle populations. By maturing at an old age, sea turtles will be less resilient to anthropogenic mortality than previously suspected.