An interspecific Nicotiana Hybrid as a useful and cost-effective platform for production of animal vaccines

The use of transgenic plants to produce novel products has great biotechnological potential as the relatively inexpensive inputs of light, water, and nutrients are utilised in return for potentially valuable bioactive metabolites, diagnostic proteins and vaccines. Extensive research is ongoing in this area internationally with the aim of producing plant-made vaccines of importance for both animals and humans. Vaccine purification is generally regarded as being integral to the preparation of safe and effective vaccines for use in humans. However, the use of crude plant extracts for animal immunisation may enable plant-made vaccines to become a cost-effective and efficacious approach to safely immunise large numbers of farm animals against diseases such as avian influenza. Since the technology associated with genetic transformation and large-scale propagation is very well established in Nicotiana, the genus has attributes well-suited for the production of plant-made vaccines. However the presence of potentially toxic alkaloids in Nicotiana extracts impedes their use as crude vaccine preparations. In the current study we describe a Nicotiana tabacum and N. glauca hybrid that expresses the HA glycoprotein of influenza A in its leaves but does not synthesize alkaloids. We demonstrate that injection with crude leaf extracts from these interspecific hybrid plants is a safe and effective approach for immunising mice. Moreover, this antigen-producing alkaloid-free, transgenic interspecific hybrid is vigorous, with a high capacity for vegetative shoot regeneration after harvesting. These plants are easily propagated by vegetative cuttings and have the added benefit of not producing viable pollen, thus reducing potential problems associated with bio-containment. Hence, these Nicotiana hybrids provide an advantageous production platform for partially purified, plant-made vaccines which may be particularly well suited for use in veterinary immunization programs.

Sources of variation in fibre diameter attributes of Australian alpacas and implications for fleece evaluation and animal selection

Sources of variation in fibre diameter attributes of Australian alpacas and implications for fleece evaluation and animal selection were investigated using data collected in the years 1994–97, from 6 properties in southern Australia. Data were analysed using REML (multiple regression analysis) to determine the effect on mean fibre diameter (MFD) and coefficient of variation of MFD (CV(FD)) of age, origin (property), sex (entire male, female), breed (Huacaya, Suri), liveweight, fibre colour, individual, and interactions of these effects. The mean (n = 100) age (range) was 4.2 years (0.1–11.9), liveweight 72.0 kg (12.0–134 kg), MFD 29.1 μm (17.7–46.6 μm), CV(FD) 24.33% (15.0–36.7%).

A number of variables affected MFD and CV(FD). MFD increased to 7.5 years of age, and correlations between MFD at 1.5 and 2 years of age with the MFD at older ages were much higher than correlations at younger ages. Fibre diameter 'blowout' (increase with age) was positively correlated with the actual MFD at ages 2 years and older. There were important effects of farm, and these effects differed with year and shearing age. Suris were coarser than Huacayas with the effect reducing with increased liveweight; there was no effect of sex. Fleeces of light shade were 1 μm finer than dark fleeces. CV(FD) declined rapidly between birth and 2 years of age, reaching a minimum at about 4 years of age and then increasing; however, CV(FD) measurements on young animals were very poor predictors of CV(FD) at older ages, and the response of CV(FD) to age differed with farm and year. Suris had a higher CV(FD) than Huacayas on most properties, and MFD, liveweight, and sex did not affect CV(FD). Fleeces of dark shade had higher CV(FD) than fleeces of light shade in 2 of the years. It is concluded that there are large opportunities to improve the MFD and CV(FD) of alpaca fibre through selection and breeding. The potential benefit is greatest from reducing the MFD and CV(FD) of fibre from older alpacas, through reducing the between-animal variation in MFD and CV(FD). Sampling alpacas at ages <2 years is likely to substantially decrease selection efficiency for lifetime fibre diameter attributes.

FTIR and thermal analysis of cashmere and other animal fibres

In this paper, sereval varieties of animal fibres including cashmere and Australian fine Merino wool have been analysed by Fourier transform infrared microscope and differential scanning calorimeter. The results showed that both Chinese and Australian cashmere fibres started absorbing heat at a relatively higher temperature but were thermally degraded quicker than other animal fibres tested. However, the mass changes (within the temperature range of 200oC to 350oC) and associated onset temperatures varied among fibre varieties. From the attenuated total reflectance spectra, the Chinese cashmere was clearly different from Australian cashmere and wool in a peak near 1040 cm-1 wavelength for S-O stretching of cysteic acid residues. The Chinese cashmere presented a stronger absorption at 1019 cm-1 wavelength, while Australian cashmere and wool peaked at 1079 cm-1 wavelength and had a weaker absorption. Combined with thermal analysis, the normalised R-SO3 - content of cysteic acid residues to the amide II peak of the protein backbone may have potential use in identifying Australian fine Merino wool from Chinese cashmere.

Effect of diets containing n-3 fatty acids on muscle long-chain n-3 fatty acid content in lambs fed low- and medium-quality roughage diets

In two experiments, each with 32 crossbred ([Merino x Border Leicester] x Poll Dorset) wether lambs (26 to 33 kg weight range), animals were randomly assigned to one of four treatments. A mixture of lucerne chaff:oaten chaff was used as a basal diet, offered in different ratios. Animals were allowed to consume on a free-access basis in Exp. 1 or 90% of ad libitum intake in Exp. 2 in order to provide a low- (6.5 MJ ME/d) and medium- (9.5 MJ ME/d) quality basal diet, respectively. Isoenergetic amounts of lipid supplements, fish meal (80 g DM), canola meal (84 g DM), and soy meal (75 g DM) were tested in Exp. 1. In Exp. 2, fish meal (9% DM), unprotected rapeseed (7% DM), and protected canola seed (6% DM) were fed as supplements. At the end of 53-d (Exp. 1) or 46-d (Exp. 2) experimental periods, lambs were slaughtered at a commercial abattoir and at 24 h postmortem longissimus thoracis (LT) muscle was collected for the analysis of fatty acid (FA) composition of structural phospholipid and storage triglyceride fractions. Fish meal diet increased LT muscle long-chain n-3 FA content by 27% (P < 0.02) in Exp. I and 30% (P < 0.001) in Exp. 2 compared with lambs fed the basal diet, but fish meal decreased (P < 0.01) the n-6 FA content only in Exp. 1. Soy meal and protected canola seed diets increased (P < 0.01) LT muscle n-6 FA content but did not affect long-chain n-3 FA content. Longissimus thoracis muscle long-chain n-3 FA were mainly deposited in structural phospholipid, rather than in storage triglyceride. In both Exp. 1 and Exp. 2, the ratio of n-6:n-3 FA in LT muscle was lowest (P < 0.01) in lambs fed fish meal supplement compared with all other treatments. Protected canola seed diet increased the ratio of n-6:n-3 FA (P < 0.01) and PUFA:saturated fatty acid (P < 0.03) content from those animals fed the basal, fish meal, and unprotected rapeseed diets in Exp. 2. This was due to an increase in muscle n-6 FA content, mainly linoleic acid, of both phospholipid (P < 0.001) and triglyceride (P < 0.01) fractions and not to an increase in muscle n3 FA content. The results indicate that by feeding fish meal supplement, the essential n-3 FA can be increased while lowering the ratio of n-6:n-3 content in lamb meat to an extent that could affect nutritional value, attractiveness, and the economic value of meat.

Palaeontology, the biogeohistory of Victoria

The broad-scale distribution of fossils within Victoria is controlled by general global patterns in the biological evolution of life on Earth, the local development and environmental evolution of habitats, and the occurrence of geological processes conducive to the preservation of fossil floras and faunas. Early Palaeozoic fossils are mostly marine in origin because of the predominance of marine sedimentary rocks in Victoria and because life on land was not significant during most of this time interval. Middle Palaeozoic sequences have both terrestrial and marine fossil records. Within Victoria, marine rocks are only very minor components of strata deposited during the late Palaeozoic, so that few marine fossils are known from this time period. A similar situation existed during most of the Mesozoic except towards the end of this era when marine conditions began to prevail in the Bass Strait region. During long intervals in the Cainozoic, large areas of Victoria were flooded by shallow-marine seas, particularly in the southern basins of Bass Strait, as well as in the northwest of the State (Murray Basin). Cainozoic sediments contain an extraordinary range of animal and plant fossils. During the Quaternary, the landscape of Victoria became, and continues to be, dominated by continental environments including, at times, extensive freshwater lake systems. Fossil floras and faunas from sediments deposited in these lake systems and from other continental sediments, as well as from Quaternary sediments deposited in marginal marine environments, collectively record a history of rapid fluctuations in climate and sea level.

Terrestrial detritus supports the food webs in lowland intermittent streams of south-eastern Australia : a stable isotope study

1. Large amounts of terrestrial detritus enter many low-order forested streams, and this organic
material is often the major basal resource in the metazoan food webs of such systems. However,
despite their apparently low biomass, algae are the dominant food of organisms in a number of
aquatic communities which conventionally would have been presumed to be dependent on
allochthonous detritus, particularly those in the tropics and also in lowland intermittent streams
in arid Australia.
2. The dual stable isotope signatures (d13C and d15N) of potential primary food sources were
compared with the isotopic signatures of common aquatic animals in lowland intermittent
streams in south-eastern Australia, in both spring and summer, to determine whether
allochthonous detritus was an important nutritional resource in these systems. The isotopic
signatures of the major potential allochthonous plant food sources (Eucalyptus, Phalaris and
Juncus) overlapped, but were distinct from algae and the dominant macrophytes growing in the
study reaches. The isotopic signatures of biofilm were more spatially and temporally variable
than those of the other basal resources.
3. Despite allochthonous detritus having relatively high C : N ratios compared to other
potential basal resources, results from ISOSOURCE mixing model calculations demonstrated
that this detritus, and the associated biofilm, were the major energy sources assimilated by
macroinvertebrate primary consumers in both spring and summer. The importance of these
energy sources was also reflected in animals higher in the food web, including predatory
macroinvertebrates and fish. These resources were supplemented by autochthonous sources of
higher nutritional value (i.e. filamentous algae and macrophytes, which had relatively low
C : N ratios) when they became more prolific as the streams dried to disconnected pools in
summer.
4. The results highlight the importance of allochthonous detritus (particularly from Eucalyptus)
as a dependable energy source for benthic macroinvertebrates and fish in lowland intermittent
streams of south-eastern Australia. This contrasts with previous stable isotope studies
conducted in lowland intermittent streams in arid Australia, which have reported that the fauna
are primarily dependent on autochthonous algae.

Protein nutrition in fibromyalgia

Background: Fibromyalgia (FM) is a soft-tissue disease of unknown origin. It causes soft-tissue pain and stiffness, often with chronic fatigue, disrupted sleep, headaches and irritable bowel. Fibromyalgia affects an estimated six million Americans of which 80 to 90 percent are female.

Objective: To determine whether dietary intake of protein, Tryptophan, and Branched Chain Amino Acids (BCAA) meet Dietary Reference Intake (DRI) recommendations, and whether there is a difference in animal and vegetable protein intake in subjects with FM compared to healthy controls.

Methods: Thirty subjects with FM and an equal number of controls completed a Food Frequency Questionnaire (FFQ) regarding dietary intake over the previous month. The FFQs were then computer analyzed to determine dietary intake.

Results: Protein intake of all subjects was more than adequate to meet DRI recommendations and there was no significant difference in intake of protein, BCAA, Tryptophan, animal or vegetable protein. Subjects with FM had significantly higher body weight and Body Mass Index (BMI) than controls, and reported having a higher incidence of Irritable Bowel Syndrome (IBS) symptoms than controls.

Conclusion: There was no significant difference in dietary intake of protein, Tryptophan, BCAA, or amounts of animal or vegetable protein in FM subjects compared to healthy controls. Significant differences in body weight and BMI in FM subjects might be related to less physical activity or possibly to malabsorption problems associated with IBS. Malabsorption related to IBS might increase the potential for protein malnutrition, FM, and associated symptoms like chronic fatigue.

Spatial memory in a food storing corvid I. Near tall landmarks are primarily used

This work suggests how food storing corvids use spatial memory to relocate caches, and how they can do this after some landmarks surrounding caches have become hidden due to leaf fall, snow fall or plant growth. Experiments involved training European jays (Garrulus glandarius) to find buried food, the location of which was specified by an array of 12 landmarks. Tests were then performed with the array rotated, or with certain landmarks removed from the array. The.main findings were: (1) birds primarily remembered the position of the goal using the near tall landmarks (15-30 cm from the goal and 20 cm high); (2) birds obtained a sense of direction both from the landmark array and something external to the array; (3) birds did not use smell or marks in the surface of the ground to find the goal. Memory of near tall landmarks is likely to be functional for these birds since (a) nearer landmarks provide a more accurate fix, and (b) taller landmarks are less likely to be completely obscured by snow fall, leaf fall or intervening vegetation. The work also demonstrates the use of G.I.S. software for the analysis and representation of animal search patterns.

Influence of stocking rate and mixed grazing of Angora goats and Merino sheep on animal and pasture production in southern Australia : 1 : botanical composition, sward characteristics and availability of components of annual temperate pastures

The effects of animal species (AS; Angora goats, Merino sheep or goats and sheep mixed grazed together at ratio 1:1) and stocking rate (SR; 7.5, 10 and 12.5 animals/ha) on the availability, botanical composition and sward characteristics of annual temperate pastures under continuous grazing were determined in a replicated experiment from 1981 to 1984. AS and SR had significant effects on pasture availability and composition and many AS SR interactions were detected. The pastures grazed by sheep had significantly reduced content and proportion of subterranean clover and more undesirable grasses compared with those grazed by goats. There were no differences in dry matter availabilities between goat- and sheep-grazed pastures at 7.5/ha, but at 10 and 12.5/ha goat pastures had significantly increased availabilities of green grass, dead and green clover and less weeds compared with sheep pastures. There was a significant AS SR interaction for the density of seedlings in May following pasture germination. Between July and January, the height of pastures was greater under goats than sheep but from January to March pasture height declined more on goat-grazed than on sheep-grazed pastures. There was an AS SR interaction for incidence of bare ground. Increasing the SR increased bare ground in pastures grazed by sheep but no change occurred on pastures grazed by goats. Changes in pasture characteristics due to increased SR were minimised on pastures grazed by goats but the grazing of sheep caused larger and faster changes and the pastures were damaged at the highest SR. Goats did not always select the same herbage material as sheep, changed their selection between seasons and were not less selective than sheep. Angora goats were flexible grazers and continually adapted their grazing behaviour to changing herbage conditions. Goat grazing led to an increase in subterranean clover, an accumulation of dead herbage at the base of the sward, reduced bare ground, taller pastures in spring and a more stable botanical composition. Mixed-grazed pasture characteristics were altered with SR. With careful management Angora goats on sheep farms may be used to manipulate pasture composition, to speed up establishment of subterranean clover, to decrease soil erosion and to reduce weed invasion.