In situ localization associates biologically active plant natriuretic peptide immuno-analogues with conductive tissue and stomata

Plant natriuretic peptide immuno-analogues (irPNP) have previously been shown to affect a number of biological processes including stomatal guard cell movements, ion fluxes and osmoticum-dependent water transport. Tissue printing and immunofluorescent labelling techniques have been used here to study the tissue and cellular localization of irPNP in ivy (Hedera helix L.) and potato (Solanum tuberosum L.). Polyclonal antibodies active against human atrial natriuretic peptide (anti-hANP) and antibodies against irPNP from potato (anti-StPNP) were used for immunolabelling. Tissue prints revealed that immunoreactants are concentrated in vascular tissues of leaves, petioles and stems. Phloem-associated cells, xylem cells and parenchymatic xylem cells showed the strongest immunoreaction. Immunofluorescent microscopy with fluorescein isothiocyanate (FITC)-conjugated goat anti-rabbit IgG supported this finding and, furthermore, revealed strong labelling to stomatal guard cells and the adjacent apoplastic space as well. Biologically active immunoreactants were also detected in xylem exudates of a soft South African perennial forest sage (Plectranthus ciliatus E. Mey ex Benth.) thus strengthening the evidence for a systemic role of the protein. In summary, in situ cellular localization is consistent with physiological responses elicited by irPNPs reported previously and is indicative of a systemic role in plant homeostasis.

Creative conservation and Balinese traditional landscape : scenarios for eco city concepts and culturally sensitive tourism development

Bali is internationally recognized as an island possessing a beautiful natural landscape as well as a unique culture. The natural qualities of its mountains, lakes, rivers, rice terrace fields with subak irrigation make Bali an important tourism destination. Cultural Tourism is integral in Bali’s tourism industry providing the basic capital for development1. The social condition of this society that is strongly characterized by religious beliefs, and its nature and ecology also supports this. The conservation and maintenance of this traditional landscape is often forgotten because of government agendas to implement cultural city programs aimed at encouraging tourism development. Despite this, the government is now supporting the program of ‘Bali toward Garden Island’, which aims to sustain the physical and cultural environment of the island towards conservation of its landscape. The implementation of this program includes attention to universal, societal and cultural values as unity indicators, of which the landscape planning of the Balinese characteristics and traditions cannot be separated. Landscape planning is integral in this initiative of character defining the region.

Globalisation is increasingly becoming one of the most important discussions amongst the Balinese people. It has become a national concern about the changes implicating Bali’s environment. Urbanisation, population growth, ribbon development, migration and consumption of energy are important imperatives and necessary evils for growing cities. These imperatives are creating the sprawl of building planning, development information, loss of open spaces, as well as the decline of the identity of cities. Places such as Denpasar City are struggling with increasing population at a rate of 1.94% per year that is causing increase in housing and public facilities demanded by both residents and ex-patriates. Thus land associated with the city has been lost to the rapid development of this cultural landscape.

This paper examines the Balinese traditional landscape and its role in encouraging tourism development that based on the Balinese culture and its ecology. The paper focuses on the planning of city landscape appearance characteristics and seeks to test and adopt the terms ‘creative conservation’ and ‘eco city concept’. By conserving the most important philosophy of the Balinese Tri Hita Karana Concept will better inform all aspects of city development in Bali. This study seeks to offer guidance for the legitimate use of landscape planning especially for city development in Bali.

Camera trapping: a contemporary approach to monitoring invasive rodents in high conservation priority ecosystems

Invasive rodent species have established on 80% of the world's islands causing significant damage to island environments. Insular ecosystems support proportionally more biodiversity than comparative mainland areas, highlighting them as critical for global biodiversity conservation. Few techniques currently exist to adequately detect, with high confidence, species that are trap-adverse such as the black rat, Rattus rattus, in high conservation priority areas where multiple non-target species persist. This study investigates the effectiveness of camera trapping for monitoring invasive rodents in high conservation areas, and the influence of habitat features and density of colonial-nesting seabirds on rodent relative activity levels to provide insights into their potential impacts. A total of 276 camera sites were established and left in situ for 8 days. Identified species were recorded in discrete 15 min intervals, referred to as 'events'. In total, 19 804 events were recorded. From these, 31 species were identified comprising 25 native species and six introduced. Two introduced rodent species were detected: the black rat (90% of sites), and house mouse Mus musculus (56% of sites). Rodent activity of both black rats and house mice were positively associated with the structural density of habitats. Density of seabird burrows was not strongly associated with relative activity levels of rodents, yet rodents were still present in these areas. Camera trapping enabled a large number of rodents to be detected with confidence in site-specific absences and high resolution to quantify relative activity levels. This method enables detection of multiple species simultaneously with low impact (for both target and non-target individuals); an ideal strategy for monitoring trap-adverse invasive rodents in high conservation areas.