Contribution of fungi to primary biogenic aerosols in the atmosphere : active discharge of spores, carbohydrates, and inorganic ions by Asco- and Basidiomycota

Spores and related chemical compounds from actively spore-discharging Ascomycota (AAM) and actively spore-discharging Basidiomycota (ABM) are primary biogenic components of air particulate matter (characteristic size range 1–10 μm). Measurement results and budget calculations based on investigations in Amazonia (Balbina, Brazil, July 2001) indicate that the forcible discharge of fungal spores may account for a large proportion of coarse air particulate matter in tropical rainforest regions during the wet season. For the particle diameter range of 1–10 μm, the estimated proportions are ~25% during day-time, ~45% at night, and ~35% on average. For the sugar alcohol, mannitol, the budget calculations indicate that it is suitable for use as a molecular tracer for actively discharged basidiospores (ABS), and that the literature-derived emission ratio of about 5 pg per ABS may be taken as a representative average. ABM emissions may account for most of the atmospheric abundance of mannitol, and can explain the observed diurnal cycle (higher abundance at night). ABM emissions of hexose carbohydrates might also account for a significant proportion of glucose and fructose in air particulate matter, but the literature-derived ratios are not consistent with the observed diurnal cycle (lower abundance at night). AAM emissions appear to account for a large proportion of potassium in air particulate matter over tropical rainforest regions during the wet season, and they can also explain the observed diurnal cycle (higher abundance at night). The results of our investigations and budget calculations for tropical rainforest aerosols are consistent with measurements performed at other locations.

Based on the average abundance of mannitol in particulate matter, which is consistent with the above emission ratio and the observed abundance of ABS, we have also calculated a value of ~17 Tg yr−1 as a first estimate for the global average emission rate of ABS over land surfaces. Comparisons with estimated rates of emission and formation of other major types of organic aerosol (~47 Tg yr−1 of anthropogenic primary organic aerosol; 12–70 Tg yr−1 of secondary organic aerosol) indicate that emissions from actively spore-discharging fungi should be taken into account as a significant source of organic aerosol. Their effects might be particularly important in tropical regions, where both physicochemical processes in the atmosphere and biological activity at the Earth's surface are particularly intense, and where the abundance of fungal spores and related chemical compounds are typically higher than in extratropical regions.

The 'wimple' : rippled deformation of a fluid drop caused by hydrodynamic and surface forces during thin film drainage

It is well-known that hydrodynamic pressures in a thin draining liquid film can cause inversion of the curvature of a drop or bubble surface as it approaches another surface, creating a so-called “dimple”. Here it is shown that a more complicated rippled shape, dubbed a “wimple”, can be formed if a fluid drop that is already close to a solid wall is abruptly pushed further toward it. The wimple includes a central region in which the film remains thin, surrounded by a ring of greater film thickness that is bounded at the outer edge by a barrier rim where the film is thin. This shape later evolves into a conventional dimple bounded by the barrier rim, which then drains in the normal way. During the evolution from wimple to dimple, some of the fluid in the thicker part of the film ring flows toward the central region before eventually draining in the opposite direction. Although the drop is pressed toward the wall, the central part of the drop moves away from the wall before approaching it again. This is observed even when the inward push is too small to create a wimple.

Influence of atmospheric helium plasma on the surface energy of jute fibres and the performance of resulting composites

In this work, an atmospheric pressure glow discharge helium plasma treatment was employed to modify the surface properties of jute fibres. The resulting bio-composites showed an increase in flexural properties and interlaminar shear strength (ILSS) compared to composites produced using untreated jute fibres. To understand the reason behind the ILSS improvement, the acid–base properties of jute fibres were determined by contact angle analysis using the capillary rise method. The results were fitted further to van Oss–Chaudhury–Good (vOCG) and Chang–Qin–Chen (CQC) models to determine the Lifshitz–van der Waals (LW) and acid–base components of surface energy. Surface energy determined by the vOCG model revealed that plasma treatment of jute fibre resulted in a 22% increase in total surface energy, a 19% increase in the LW component and a 24% increase in the acid–base component of surface energy. The increase in the acid–base component is due to the significant increase (69%) in the electron-accepting (γ+S) parameter. On the other hand, the CQC model clearly indicates an amphoteric nature of the fibre surface based on opposite signs of the acid and base principal values (PSa and PSb). Overall, the results indicated that increases in both LW and acid–base components were responsible for improvement in the properties of the composites.

One step multifunctional micropatterning of surfaces using asymmetric glow discharge plasma polymerization

Micropatterning of surfaces with varying chemical, physical and topographical properties usually requires a number of fabrication steps. Herein, we describe a micropatterning technique based on plasma enhanced chemical vapour deposition (PECVD) that deposits both protein resistant and protein repellent surface chemistries in a single step. The resulting multifunctional, selective surface chemistries are capable of spatially controlled protein adhesion, geometric confinement of cells and the site specific confinement of enzyme mediated peptide self-assembly.

Influence of surface mechanical attrition treatment attrition media on the surface contamination and corrosion of magnesium

Surface mechanical attrition treatment (SMAT) is a mechanical peening process used to generate ultrafine grain surfaces on a metal. SMAT was carried out on pure magnesium using different attrition media (zirconia [ZiO2], alumina [Al2O3], and steel balls) to observe the effect on microstructure, surface residual stress, surface composition, and corrosion. Surface contamination from SMAT was characterized using glow discharge optical emission spectroscopy (GDOES). The SMAT process produced a refined grain structure on the surface of Mg but resulted in a region of elemental contamination extending ~10 μm into the substrate, regardless of the media used. Consequently, SMAT-treated surfaces showed an increased corrosion rate compared to untreated Mg, primarily through increased cathodic kinetics. This study highlights the issue of contamination resulting from the SMAT process, which is a penalty that accompanies the significant grain refinement of the surface produced by SMAT. This must be considered if attempting to exploit grain refinement for improving corrosion resistance.

Comparison between response surface models and artificial neural networks in hydrologic forecasting

Developing an efficient and accurate hydrologic forecasting model is crucial to managing water resources and flooding issues. In this study, response surface (RS) models including multiple linear regression (MLR), quadratic response surface (QRS), and nonlinear response surface (NRS) were applied to daily runoff (e.g., discharge and water level) prediction. Two catchments, one in southeast China and the other in western Canada, were used to demonstrate the applicability of the proposed models. Their performances were compared with artificial neural network (ANN) models, trained with the learning algorithms of the gradient descent with adaptive learning rate (ANN-GDA) and Levenberg-Marquardt (ANN-LM). The performances of both RS and ANN in relation to the lags used in the input data, the length of the training samples, long-term (monthly and yearly) predictions, and peak value predictions were also analyzed. The results indicate that the QRS and NRS were able to obtain equally good performance in runoff prediction, as compared with ANN-GDA and ANN-LM, but require lower computational efforts. The RS models bring practical benefits in their application to hydrologic forecasting, particularly in the cases of short-term flood forecasting (e.g., hourly) due to fast training capability, and could be considered as an alternative to ANN

Differing impact of a major biogeographic barrier on genetic structure in two large kangaroos from the monsoon tropics of Northern Australia

Tropical savannas cover 20-30% of the world's land surface and exhibit high levels of regional endemism, but the evolutionary histories of their biota remain poorly studied. The most extensive and unmodified tropical savannas occur in Northern Australia, and recent studies suggest this region supports high levels of previously undetected genetic diversity. To examine the importance of barriers to gene flow and the environmental history of Northern Australia in influencing patterns of diversity, we investigated the phylogeography of two closely related, large, vagile macropodid marsupials, the antilopine wallaroo (Macropus antilopinus; n = 78), and the common wallaroo (Macropus robustus; n = 21). Both species are widespread across the tropical savannas of Australia except across the Carpentarian Barrier (CB) where there is a break in the distribution of M. antilopinus. We determined sequence variation in the hypervariable Domain I of the mitochondrial DNA control region and genotyped individuals at 12 polymorphic microsatellite loci to assess the historical and contemporary influence of the CB on these species. Surprisingly, we detected only limited differentiation between the disjunct Northern Territory and QueenslandM. antilopinus populations. In contrast, the continuously distributedM. robustus was highly divergent across the CB. Although unexpected, these contrasting responses appear related to minor differences in species biology. Our results suggest that vicariance may not explain well the phylogeographic patterns in Australia's dynamic monsoonal environments. This is because Quaternary environmental changes in this region have been complex, and diverse individual species' biologies have resulted in less predictable and idiosyncratic responses.

Characterization and optimization to improve uneven surface on MEMS bridge fabrication

This paper presents an optimized fabrication method for developing a freestanding bridge for RF MEMS switches. In this method, the sacrificial layer is patterned and hard baked a 220°C for 3min, after filling the gap between the slots of the coplanar waveguide. Measurement results by AFM and SEM demonstrate that this technique significantly improves the planarity of the sacrificial layer, reducing the uneven surface to less than 20nm, and the homogeneity of the Aluminum thickness across the bridge. Moreover, a mixture of O2, Ar and CF4 was used and optimized for dry releasing of the bridge. A large membrane (200×100μm2) was released without any surface bending. Therefore, this method not only simplifies the fabrication process, but also improves the surface flatness and edge smoothness of the bridge. This fabrication method is fully compatible with standard silicon IC technology.

The effect of treatment time on the ionic liquid surface film formation: promising surface coating for Mg alloy AZ31

Mg alloys are attractive materials for medical devices. The main limitation is that they are prone to corrosion. A low toxicity surface coating that enables uniform, controlled corrosion at a desired rate (this usually means it must offer barrier functions for a limited time period) is desirable. Phosphate-based ionic liquids (ILs) are known to induce a coating that can reduce the corrosion rate of Mg alloys, Furthermore, some ILs are known to be biocompatible and therefore, controlling the corrosion behaviour of an Mg alloy and its surface biocompatibility can be achieved through adding an appropriate low toxic IL surface layer to the substrate. In this study, we have evaluated the cytotoxicity of three phosphate-based ILs to primary human coronary artery endothelial cells. Among them, tributyl(methyl)-phosphonium diphenylphosphate (P1,4,4,4dpp) shows the lowest cytotoxicity. Therefore, further work was aimed at developing an appropriate treatment method to produce a homogeneous and passive surface coating based on P1,4,4,4dpp IL, with the focus on investigating the effect of treatment time. The results showed that that the formation of IL coating on AZ31 has proceeded progressively, and treatment time plays an important role. An IL treatment at 100 °C with an extended treatment time of 5 h significantly enhanced corrosion resistance of the AZ31 alloy in simulated body fluid. Additionally, the corrosion morphology was uniform and there was no evidence of "localized pitting corrosion" observed. Such a performance makes this ionic liquid coating as a potential surface coating biodegradable Mg-based implants.

Effect of cold plasma pre-treatment on photocatalytic activity of 3D fabric loaded with nano-photocatalysts: response surface methodology

In this study, the physico-chemical effects occasioned by the cold plasma discharge (CPD) on the photo-decolorization of Reactive Orange 16 (RO16) by 3D fabrics (spacer fabrics) loaded with ZnO:TiO2 nano-photocatalysts (nphs) were optimized via response surface methodology (RSM). CPD was employed to improve the surface characteristics of the spacer fabrics for nphs loading. Surface morphology and color variation were studied utilizing scanning electron microscopy (SEM) and CIE-Lab system, respectively. The effect of CPD on the wetting ability of the spacer fabrics was examined using dynamic adsorption measurement (DAM). Also, X-ray fluorescence (XRF) was utilized to investigate the durability of the nphs on the spacer fabrics. All the experiments were implemented in a Box-Behnken design (BBD) with three independent variables (CPD treatment time, dye concentration and irradiation time) in order to optimize the decolorization of RO16. The anticipated values of the decolorization efficiency were found to be in excellent agreement with the experimental values (R2 = 0.9996, Adjusted R2 = 0.9992). The kinetic analysis demonstrated that the photocatalytic decolorization followed the Langmuir-Hinshelwood kinetic model. In conclusion, this heterogeneous photocatalytic process is capable of decolorizing and mineralizing azoic reactive dye in textile wastewater. Moreover, the results confirmed that RSM based on the BBD was a suitable method to optimize the operating conditions of RO16 degradation.

Boron nitride nanosheet-veiled gold nanoparticles for surface-enhanced raman scattering

Atomically thin boron nitride (BN) nanosheets have many properties desirable for surface-enhanced Raman spectroscopy (SERS). BN nanosheets have a strong surface adsorption capability toward airborne hydrocarbon and aromatic molecules. For maximized adsorption area and hence SERS sensitivity, atomically thin BN nanosheet-covered gold nanoparticles have been prepared for the first time. When placed on top of metal nanoparticles, atomically thin BN nanosheets closely follow their contours so that the plasmonic hot spots are retained. Electrically insulating BN nanosheets also act as a barrier layer to eliminate metal-induced disturbances in SERS. Moreover, the SERS substrates veiled by BN nanosheets show an outstanding reusability in the long term. As a result, the sensitivity, reproducibility, and reusability of SERS substrates can be greatly improved. We also demonstrate that large BN nanosheets produced by chemical vapor deposition can be used to scale up the proposed SERS substrate for practical applications.

Electrical discharge machining of 6061 aluminium alloy

The wire electrical discharge machining (EDM) of 6061 aluminium alloy in terms of material removal rate, kerf/slit width, surface finish and wear of electrode wire for different pulse on time and wire tension was studied. Eight experiments were carried out in a wire EDM machine by varying pulse on time and wire tension. It is found that the material removal rate increases with the increase of pulse on time though the wire tension does not affect the material removal rate. It seems that the higher wire tension facilitates steady machining process, which generates low wear in wire electrode and better surface finish. The surface roughness does not change notably with the variation of pulse on time. The appearance of the machined surfaces is very similar under all the machining conditions. The machined surface contains solidified molten material, splash of materials and blisters. The increase of the pulse on time increases the wear of wire electrode due to the increase of heat input. The wear of wire electrode generates tapered slot which has higher kerf width at top side than that at bottom side. The higher electrode wear introduces higher taper.

Bimodal magneto-luminescent dysprosium (DyIII)-potassium (KI)-oxalate framework: magnetic switchability with high anisotropic barrier and solvent sensing

We report synthesis, characterization, and properties of a multifunctional oxalate framework, {KDy(C₂O₄)₂(H₂O)₄}_n (1) (C₂O₄^2- = oxalate dianion) composed of two absolutely different metal ions in terms of their size, charge, and electronic configuration. Dehydrated framework (1′) exhibits permanent porosity and interesting solvent (H₂O, MeOH, CH₃CN, and EtOH) vapor sorption characteristics based on specific interactions with unsaturated alkali metal sites on the pore surface. Compound 1 shows solvent responsive bimodal magnetic and luminescence properties related to the DyIII center. Compound 1 exhibits reversible ferromagnetic to antiferromagnetric phase transition upon dehydration and rehydration, hitherto unknown for any lanthanide based coordination polymer or metal-organic frameworks. Both the compounds 1 and 1′ exhibit slow magnetic relaxation with very high anisotropic barrier (417 ± 9 K for 1 and 418 ± 7 K for 1′) which has been ascribed to the single ion magnetic anisotropy of the Dy^III centers. Nevertheless, compound 1 shows a metal based luminescence property in the visible region and H₂O molecules exhibit the strongest quenching effect compared to other solvents MeOH, MeCN, and EtOH, evoking 1′ as a potential H₂O sensor.