Cellular localization of water soluble, allergenic proteins in rye-grass (Lolium perenne) pollen using monoclonal and specific IgE antibodies with immunogold probes

A postembedding method has been developed for localizing water soluble allergens in rye-grass pollen. This uses dry fixation in glutaraldehyde vapour, followed by 2,2-dimethoxypropane, prior to a 100% ethanol series leading into embedment in LR Gold. This has allowed the attachment of specific monoclonal antibodies to the allergen, which are themselves probed with specific immunogold labels to the antibodies. Wall and cytoplasmic sites have been identified, representing an improvement of fixation and localization of allergens over previous studies employing polyclonal, broad spectrum antibodies.

Rye-grass allergens are labelled in mature pollen grains in the exine (tectum, nexine and central chamber), and in the electron opaque areas of the cytoplasm, especially mitochondria. The allergens are absent from the intine, polysaccharide (P) particles, amyloplasts, Golgi bodies and endoplasmic reticulum. IgE antibodies derived from humans allergic to rye-grass pollen, bind to similar sites in the cytoplasm but only to the outer surface of the pollen grain wall. This method now provides a valuable tool for further developmental studies on the pollen grains, in order to establish the site/s of synthesis of the allergens.

Visual tracking of vehicles using multiresolution analysis and neural network

This paper describes the procedure for detection and tracking of a vehicle from an on-road image sequence taken by a monocular video capturing device in real time. The main objective of such a visual tracking system is to closely follow objects in each frame of a video stream, such that the object position as well as other geometric information are always known. In the tracking system described, the video capturing device is also moving. It is a challenge to detect and track a moving vehicle under a constantly changing environment coupled to real time video processing. The system suggested is robust to implement under different illuminating conditions by using the monocular video capturing device. The vehicle tracking algorithm is one of the most important modules in an autonomous vehicle system, not only it should be very accurate but also must have the safety of other vehicles, pedestrians, and the moving vehicle itself. In order to achieve this an algorithm of multi resolution technique based on Haar basis functions were used for the wavelet transform, where a combination of classification was carried out with the multilayer feed forward neural network. The classification is done in a reduced dimensional space, where principle component analysis (PCA) dimensional reduction technique has been applied to make the classification process much more efficient. The results show the effectiveness of the proposed methodology.

Differential expression of ATP7A, ATP7B and CTR1 in adult rat dorsal root ganglion tissue

Background: ATP7A, ATP7B and CTR1 are metal transporting proteins that control the cellular disposition of copper and platinum drugs, but their expression in dorsal root ganglion (DRG) tissue and their role in platinum-induced neurotoxicity are unknown. To investigate the DRG expression of ATP7A, ATP7B and CTR1, lumbar DRG and reference tissues were collected for real time quantitative PCR, RT-PCR, immunohistochemistry and Western blot analysis from healthy control adult rats or from animals treated with intraperitoneal oxaliplatin (1.85 mg/kg) or drug vehicle twice weekly for 8 weeks.
Results: In DRG tissue from healthy control animals, ATP7A mRNA was clearly detectable at levels similar to those found in the brain and spinal cord, and intense ATP7A immunoreactivity was localised to the cytoplasm of cell bodies of smaller DRG neurons without staining of satellite cells, nerve fibres or co-localisation with phosphorylated heavy neurofilament subunit (pNF-H). High levels of CTR1 mRNA were detected in all tissues from healthy control animals, and strong CTR1 immunoreactivity was associated with plasma membranes and vesicular cytoplasmic structures of the cell bodies of larger-sized DRG neurons without co-localization with ATP7A. DRG neurons with strong expression of ATP7A or CTR1 had distinct cell body size profiles with minimal overlap between them. Oxaliplatin treatment did not alter the size profile of strongly ATP7A-immunoreactive neurons but significantly reduced the size profile of strongly CTR1-immunoreactive neurons. ATP7B mRNA was barely detectable, and no specific immunoreactivity for ATP7B was found, in DRG tissue from healthy control animals.
Conclusions: In conclusion, adult rat DRG tissue exhibits a specific pattern of expression of copper transporters with distinct subsets of peripheral sensory neurons intensely expressing either ATP7A or CTR1, but not both or ATP7B. The neuron subtype-specific and largely non-overlapping distribution of ATP7A and CTR1 within rat DRG tissue may be required to support the potentially differing cuproenzyme requirements of distinct subsets of sensory neurons, and could influence the transport and neurotoxicity of oxaliplatin.

Tomorrow’s car – for today’s people : can tilting three wheeled vehicles be a solution for the problems of today and the future?

The current automotive industry and todays car drivers are faced with every increasing challenges, not previously experienced. Climate Change, financial issues, rising fuel prices, increased traffic congestion and reduced parking space in cities are all leading to changes in consumer preferences and the requirements of modern passenger vehicles. However, despite the shift in the industry dynamics, the principal layout of a car hasn’t changed since its invention. The design of a ’conventional’ vehicle is still principally a matchbox with four wheels, one at each corner. The concept has served its purpose well for over 100 years, but such a layout is not suited to solving today’s problems. To address the range of problems faced by the industry, a number of alternative commuting vehicles have been developed. Yet the commercialization of these ‘alternative’ vehicles has yet to be successful. This is largely due failure of these vehicles to meet the changing demands of the industry and the limited understanding of consumer behaviour, motivation and attitudes. Deakin University’s Tomorrow’s Car concept tackles all of these problems. The vehicle is a novel three-wheeler cross over concept between a car and a motorbike that combines the best of both worlds. The vehicle combines the low cost, small size and ‘fun’ factor of a motorbike together with the safety, comfort and easy to drive features of a car produce a vehicle with a fuel efficiency better than either car or scooter. Intensive market research has been conducted for various major potential markets of alternative vehicles including India, China and Australia. The research analysed consumer attitudes in relation to narrow tilting vehicles, and in particular towards Deakin’s Tomorrow’s Car (TC). The study revealed that a relatively large percentage of consumers find such a concept very appealing. For the other consumers, the overall appearance and perception of safety and not the actual safety performance were found to be the most impeding factors of such vehicles. By addressing these issues and marketing the vehicle accordingly the successful commercialization of Tomorrow’s Car can be ensured.

A qualitative exploration of body image experiences of women progressing through pregnancy

BACKGROUND: Pregnancy provides an interesting challenge to body image theories in that the natural physiological changes push women further from the socioculturally prescribed thin ideal which these theories hinge upon. The impact that these significant physiological changes have on the woman's body image during pregnancy may depend on the extent to which they retain or revise the ideal. However, little is known about body image experiences during pregnancy. AIM: To provide a comprehensive exploration of the body image experiences of pregnant women. METHODS: Individual structured interviews were conducted with 19 currently pregnant women. Transcriptions were analysed using a thematic content analysis approach. FINDINGS: Themes extracted from the qualitative data included: (1) women's body image experiences during pregnancy were complex and changing, and shaped by the salience of specific body parts, the women's expectations for future changes to their body within the perinatal period, the functionality of the body, and their experience of maternity clothing, (2) women were able to negotiate the changes to their bodies as they recognised the functionality of the pregnant body, (3) women were surprised by the public nature of the pregnant body, (4) partner support and positive feedback about the pregnant body was highly valued, and (5) the importance of open communication around weight and body image in antenatal healthcare. DISCUSSION: Our findings highlight the need for the adaptation and expansion of existing body image theories to be used as a framework for women's experiences of pregnancy.

Organ trafficking and transplant tourism: the role of global professional ethical standards - the 2008 declaration of Istanbul

By 2005, human organ trafficking, commercialization, and transplant tourism had become a prominent and pervasiveinfluence on transplantation therapy. The most common source of organs was impoverished people in India,Pakistan, Egypt, and the Philippines, deceased organ donors in Colombia, and executed prisoners in China. Inresponse, in May 2008, The Transplantation Society and the International Society of Nephrology developed theDeclaration of Istanbul on Organ Trafficking and Transplant Tourism consisting of a preamble, a set of principles, anda series of proposals. Promulgation of the Declaration of Istanbul and the formation of the Declaration of IstanbulCustodian Group to promote and uphold its principles have demonstrated that concerted, strategic, collaborative,and persistent actions by professionals can deliver tangible changes. Over the past 5 years, the Declaration of IstanbulCustodian Group organized and encouraged cooperation among professional bodies and relevant international, regional,and national governmental organizations, which has produced significant progress in combating organ traffickingand transplant tourism around the world. At a fifth anniversary meeting in Qatar in April 2013, the DICGtook note of this progress and set forth in a Communique´ a number of specific activities and resolved to furtherengage groups from many sectors in working toward the Declaration’s objectives.

Static output feedback frequency stabilization of time-delay power systems with coordinated electric vehicles state of charge control

In this paper, for the first time, electric vehicles are used for both the primary and secondary frequency controls to support power plants to rapidly suppress fluctuations in the system frequency due to load disturbances. Via networked control and wide-area communication infrastructures, multiple interval time-varying delays exist in the communication channels between the control center, power plant, and an aggregation of electric vehicles. By coordinating batteries’ state of charge control, the behaviors of the vehicle owners and the uncertainties imposed by the changes of the batteries’ state of charge are taken intoconsideration. A power system model incorporating multiple time-varying delays and uncertainties is first proposed. Then, a robust static output feedback frequency controller is designed to guarantee the resulting closed-loop system stable with an H∞ attenuation level. By utilizing a novel integral inequality, namely refined-Jensen inequality, and an improved reciprocally convex combination, the design conditions are formulated in terms of tractable linear matrix inequalities which can be efficiently solved by various computational tools. The effectiveness of the proposed control scheme is verified by extensive simulations.