Gestion de la rareté de l’eau à Amman : rationnement de l’offre et pratiques des usagers

Notre article aborde la question du rationnement de l’offre d’eau potable dans la capitale jordanienne Amman. Il étudie la gestion publique-privée du rationnement, ainsi que ses impacts socio-économiques sur les usagers. Si 98 % de la population jordanienne jouit d’un raccordement au réseau d’eau potable, cet accès demeure assujetti à une intermittence en quantité et en qualité. Une situation expliquée par le discours gouvernemental, qui qualifie les villes du pays d’assoiffées, par la rareté hydrique et la croissance démographique. Or, l’étude du cas d’Amman, à travers la délégation, de 1999 à 2006, de la gestion du service au secteur privé, montre que le rationnement de l’offre incombe de façon importante à l’état technique du réseau défaillant, aggravé par les pratiques de connexions illégales et le gaspillage. Cet article met en outre l’accent sur les inégalités dans les usages de l’eau entre pauvres et riches, accrues par le rationnement inadapté aux caractéristiques socio-économiques des ménages. Ainsi, le coût des stratégies alternatives pour un accès adéquat à l’eau devient inabordable pour l’ensemble des familles, confrontées à une tarification progressive dont les effets sont pervers, et met en avant le rôle discriminatoire du revenu.

A suite of parallel vectors for baculovirus expression

The expression of proteins using recombinant baculoviruses is a mature and widely used technology. However, some aspects of the technology continue to detract from high throughput use and the basis of the final observed expression level is poorly understood. Here, we describe the design and use of a set of vectors developed around a unified cloning strategy that allow parallel expression of target proteins in the baculovirus system as N-terminal or C-terminal fusions. Using several protein kinases as tests we found that amino-terminal fusion to maltose binding protein rescued expression of the poorly expressed human kinase Cot but had only a marginal effect on expression of a well-expressed kinase IKK-2. In addition, MBP fusion proteins were found to be secreted from the expressing cell. Use of a carboxyl-terminal GFP tagging vector showed that fluorescence measurement paralleled expression level and was a convenient readout in the context of insect cell expression, an observation that was further supported with additional non-kinase targets. The expression of the target proteins using the same vectors in vitro showed that differences in expression level were wholly dependent on the environment of the expressing cell and an investigation of the time course of expression showed it could affect substantially the observed expression level for poorly but not well-expressed proteins. Our vector suite approach shows that rapid expression survey can be achieved within the baculovirus system and in addition, goes some way to identifying the underlying basis of the expression level obtained. (c) 2006 Elsevier Inc. All rights reserved.

Self-esteem and coping in children with developmental coordination disorder

This study investigated self-esteem in children with developmental coordination disorder (DCD). Fifteen children between the ages of 8 and 12 years diagnosed with DCD were compared with a typically developing group comprising 30 children with average and good motor abilities, using measures of perceived competence, social support and self-esteem. The types of coping strategy generated in response to example vignettes were also compared. There was no significant difference between the groups in global self-esteem, but the children with DCD reported lower athletic and scholastic competence than their typically developing peers. No difference was found between the groups in level of perceived social support. The DCD group generated fewer coping strategies overall, but more passive and avoidant strategies than the typically developing children. The implications of the study are discussed with regard to future research directions, such as the investigation of the effects of motor skill intervention on self-esteem and the development of strategies to protect children's self-esteem.

Case studies to demonstrate the range of applications of the Southampton Hand Assessment Procedure

The Southampton Hand Assessment Procedure (SHAP) was devised to assess quantitatively the functional range of injured and healthy adult hands. It was designed to be a practical tool for use in a busy clinical setting; thus, it was made simple to use and easy to interpret. This paper describes four examples of its use: before and after a surgical procedure, to observe the impact of an injury, use with prostheses, and during recovery following a fracture. The cases show that the SHAP is capable of monitoring progress and recovery, identifying functional abilities in prosthetic hands and comparing the capabilities of different groups of injuries.