Virulence in the chick model and stress tolerance of Salmonella enterica serovar Orion var. 15+

Three Salmonella enterica serovar Orion var. 15+ isolates of distinct provenance were tested for survival in various stress assays. All were less able to survive desiccation than a virulent S. Enreritidis strain, with levels of survival similar to a rpoS mutant of the S. Enteritidis strain, whereas one isolate (F3720) was significantly more acid tolerant. The S. Orion var. 15+ isolates were motile by flagellae and elaborated type-1 and curli-like fimbriae; surface organelles that are considered virulence determinants in Salmonella pathogenesis. Each adhered and invaded HEp-2 tissue culture cells with similar proficiency to the S. Enteritidis control but were significantly less virulent than S. En teritidis in the one-day-old and seven-day-old chick model. Given an oral dose of 1 x 10(3) cfu to one-day-old chicken, S. Orion var. 15+ isolates colonised 25% of liver and spleens examined at 24 h whereas S. Enteritidis colonised 100% of organs by the same with the same dose. Given an oral dose of 1 x 10(7) cfu at seven-day old, S. Orion var. 15+ failed to colonise livers and spleens in any bird examined at 24 h whereas S. Enteritidis colonised 50% of organs by the same with the same dose. Based on the number of internal organs colonised, one of the three S. Orion var. 15+ isolates tested (strain F3720) was significantly more invasive than the other two (B1 and B7). Also, strain F3720 was shed less than either B1 or B7 supporting the concept that there may be an inverse relationship between the ability to colonise deep tissues and to persist in the gut. These data are discussed in the light that S. Orion var. 15+ is associated with sporadic outbreaks of human infection rather than epidemics.

The storms of war

The Storms of War, is the first in a trilogy about the de Witt family. The first explores their lives from 1914-1918, as the youngest girl, Celia, sees her perfect world crumble and change. Inspired to write about the wars since Kate visited the trenches in France when she was ten on a school trip. Kate was fascinated by how small they were - and how men could ever live in such places. She really wanted to go into the lives of Germans - the Victorians couldn't get enough of them. Then - almost overnight - they were the enemy and people saw German spies everywhere and the newspapers demanded that all Germans in the country be imprisoned. At the beginning of the book, Rudolf and Verena have four children - and their lives will never be the same again.

The edge of the fall

Edge of the Fall, is about the DeWitt family in the 1920s as they try to make sense of their lives in the aftermath of the war. It's the Flapper Age - and everything is in flux. A sequel to The Storms of War.