Intracellular or intercellular localization of the polar pathway of penetration across stratum corneum

A pharmacokinetic hypothesis of stratum corneum with two parallel pathways, lipophilic and porous hydrophilic, is not well documented yet. Still questionable is the localization of the pores, and the present experiments were designed to elucidate the contribution of extracellular lipids and intracellular keratin to the structure of this pathway. Percutaneous penetration of baclofen, a model zwitterion, was studied in vitro using human cadaver skin. Aqueous or ethanolic saturated solutions of the drug (Cs = 4.6 and 0.4 mg/ mL, respectively) were applied on the skin that was pretreated with: methanol/chloroform (Me/Ch) or acetone-chloroform (Ac/Ch) (1:1) mixtures, or with these solvents followed by 0.2% solution of sodium lauryl sulfate (SLS). As controls, baclofen penetration through the intact full-thickness skin was determined, and the fluxes were 0.18 ±0.08 and 0.14 ±0.07 µg/cm2/h for aqueous and ethanolic solutions, respectively. When Me/Ch was used for 1 h, an expected increase of the penetration was observed, but the lag time, Tlag, was still nearly 20 h. When the less polar mixture, Ac/Ch, was used, no flux enhancement was observed, and with ethanol as the vehicle, decreased penetration was even noted. No effect on baclofen penetration was observed when SLS was used for 1 h after delipidization of the skin was done with either the Me/Ch or Ac/Ch mixture. The results suggest that the polar pathway may be located intercellularly and comprises aqueous regions surrounded by polar lipids, which create the walls of such microchannels.

The nature of Arctic polar vortices in chemistry-climate models

The structure of the Arctic stratospheric polar vortex in three chemistry–climate models (CCMs) taken from the CCMVal-2 intercomparison is examined using zonal mean and geometric-based methods. The geometric methods are employed by taking 2D moments of potential vorticity fields that are representative of the polar vortices in each of the models. This allows the vortex area, centroid location and ellipticity to be determined, as well as a measure of vortex filamentation. The first part of the study uses these diagnostics to examine how well the mean state, variability and extreme variability of the polar vortices are represented in CCMs compared to ERA-40 reanalysis data, and in particular for the UMUKCA-METO, NIWA-SOCOL and CCSR/NIES models. The second part of the study assesses how the vortices are predicted to change in terms of the frequency of sudden stratospheric warmings and their general structure over the period 1960–2100. In general, it is found that the vortices are climatologically too far poleward in the CCMs and produce too few large-scale filamentation events. Only a small increase is observed in the frequency of sudden stratospheric warming events from the mean of the CCMVal-2 models, but the distribution of extreme variability throughout the winter period is shown to change towards the end of the twentyfirst century.

Changes in area and geodetic mass balance of small glaciers, polar Urals, Russia, 1950-2008

Changes in area of 30 small glaciers (mostly <1 km2) in the northern Polar Urals (67.5-68.25 °N) between 1953 and 2000 were assessed using historic aerial photography from 1953 and 1960, ASTER and panchromatic Landsat ETM+ imagery from 2000, and data from 1981 and 2008 terrestrial surveys. Changes in volume and geodetic mass balance of IGAN and Obruchev glaciers were calculated using data from terrestrial surveys in 1963 and 2008. In total, glacier area declined by 22.3 ± 3.9% in the 1953/60-2000 period. The areas of individual glaciers decreased by 4-46%. Surfaces of Obruchev and IGAN glaciers lowered by 22.5 ± 1.7 m and 14.9 ± 2.1 m. Over 45 years, geodetic mass balances of Obruchev and IGAN glaciers were -20.66 ± 2.91 and -13.54 ± 2.57 m w.e. respectively. Glacier shrinkage in the Polar Urals is related to a summer warming of 1 °C between 1953-81 and 1981-2008 and its rates are consistent with other regions of northern Asia but are higher than in Scandinavia. While glacier shrinkage intensified in the 1981-2000 period relative to 1953-81, increasing winter precipitation and shading effects slowed glacier wastage in 2000-08.

A comparison of two identification and tracking methods for polar lows

In this study, we compare two different cyclone-tracking algorithms to detect North Atlantic polar lows, which are very intense mesoscale cyclones. Both approaches include spatial filtering, detection, tracking and constraints specific to polar lows. The first method uses digital bandpass-filtered mean sea level pressure (MSLP) fieldsin the spatial range of 200�600 km and is especially designed for polar lows. The second method also uses a bandpass filter but is based on the discrete cosine transforms (DCT) and can be applied to MSLP and vorticity fields. The latter was originally designed for cyclones in general and has been adapted to polar lows for this study. Both algorithms are applied to the same regional climate model output fields from October 1993 to September 1995 produced from dynamical downscaling of the NCEP/NCAR reanalysis data. Comparisons between these two methods show that different filters lead to different numbers and locations of tracks. The DCT is more precise in scale separation than the digital filter and the results of this study suggest that it is more suited for the bandpass filtering of MSLP fields. The detection and tracking parts also influence the numbers of tracks although less critically. After a selection process that applies criteria to identify tracks of potential polar lows, differences between both methods are still visible though the major systems are identified in both.

The effect of climate change on the variability of the Northern Hemisphere stratospheric polar vortex

With extreme variability of the Arctic polar vortex being a key link for stratosphere–troposphere influences, its evolution into the twenty-first century is important for projections of changing surface climate in response to greenhouse gases. Variability of the stratospheric vortex is examined using a state-of-the-art climate model and a suite of specifically developed vortex diagnostics. The model has a fully coupled ocean and a fully resolved stratosphere. Analysis of the standard stratospheric zonal mean wind diagnostic shows no significant increase over the twenty-first century in the number of major sudden stratospheric warmings (SSWs) from its historical value of 0.7 events per decade, although the monthly distribution of SSWs does vary, with events becoming more evenly dispersed throughout the winter. However, further analyses using geometric-based vortex diagnostics show that the vortex mean state becomes weaker, and the vortex centroid is climatologically more equatorward by up to 2.5°, especially during early winter. The results using these diagnostics not only characterize the vortex structure and evolution but also emphasize the need for vortex-centric diagnostics over zonally averaged measures. Finally, vortex variability is subdivided into wave-1 (displaced) and -2 (split) components, and it is implied that vortex displacement events increase in frequency under climate change, whereas little change is observed in splitting events.

The information content of REIT credit rating actions and transparency

We examine the short-run and long-run price reaction of equity REIT shares following credit rating actions, testing the transparency of the REIT structure. Generally, the economic effect on the stock price is subdued for both upgrades and downgrades compared to prior literature examining the broader U.S. equity market. An examination of trading volume revealed a significant increase in trading in reaction to downgrade credit rating changes, with a more subdued response to upgrades. The findings support the notion that REITs are more publicly forthcoming about the expectation of positive news in comparison to negative news.

Neural activation and functional connectivity during motor imagery of bimanual everyday actions

Bimanual actions impose intermanual coordination demands not present during unimanual actions. We investigated the functional neuroanatomical correlates of these coordination demands in motor imagery (MI) of everyday actions using functional magnetic resonance imaging (fMRI). For this, 17 participants imagined unimanual actions with the left and right hand as well as bimanual actions while undergoing fMRI. A univariate fMRI analysis showed no reliable cortical activations specific to bimanual MI, indicating that intermanual coordination demands in MI are not associated with increased neural processing. A functional connectivity analysis based on psychophysiological interactions (PPI), however, revealed marked increases in connectivity between parietal and premotor areas within and between hemispheres. We conclude that in MI of everyday actions intermanual coordination demands are primarily met by changes in connectivity between areas and only moderately, if at all, by changes in the amount of neural activity. These results are the first characterization of the neuroanatomical correlates of bimanual coordination demands in MI. Our findings support the assumed equivalence of overt and imagined actions and highlight the differences between uni- and bimanual actions. The findings extent our understanding of the motor system and may aid the development of clinical neurorehabilitation approaches based on mental practice.

Spatio-temporal variation in predation by urban domestic cats (Felis catus) and the acceptability of possible management actions in the UK

Urban domestic cat (Felis catus) populations can attain exceedingly high densities and are not limited by natural prey availability. This has generated concerns that they may negatively affect prey populations, leading to calls for management. We enlisted cat-owners to record prey returned home to estimate patterns of predation by free-roaming pets in different localities within the town of Reading, UK and questionnaire surveys were used to quantify attitudes to different possible management strategies. Prey return rates were highly variable: only 20% of cats returned ≥4 dead prey annually. Consequently, approximately 65% of owners received no prey in a given season, but this declined to 22% after eight seasons. The estimated mean predation rate was 18.3 prey cat−1 year−1 but this varied markedly both spatially and temporally: per capita predation rates declined with increasing cat density. Comparisons with estimates of the density of six common bird prey species indicated that cats killed numbers equivalent to adult density on c. 39% of occasions. Population modeling studies suggest that such predation rates could significantly reduce the size of local bird populations for common urban species. Conversely, most urban residents did not consider cat predation to be a significant problem. Collar-mounted anti-predation devices were the only management action acceptable to the majority of urban residents (65%), but were less acceptable to cat-owners because of perceived risks to their pets; only 24% of cats were fitted with such devices. Overall, cat predation did appear to be of sufficient magnitude to affect some prey populations, although further investigation of some key aspects of cat predation is warranted. Management of the predation behavior of urban cat populations in the UK is likely to be challenging and achieving this would require considerable engagement with cat owners.

Environmental regulation and colonization attributes of the long polar fimbriae (LPF) of Escherichia coli O157:H7

The ability of Escherichia coli O157:H7 to colonize the intestinal epithelia is dependent on the expression of intimin and other adhesins. The chromosome of E. coli O157:H7 carries two loci encoding long polar fimbriae (LPF). These fimbriae mediate adherence to epithelial cells and are associated with colonization of the intestine. In order to increase our knowledge about the conditions controlling their expression and their role in colonization of an animal model, the environmental cues that promote expression of lpf genes and the role of E. coli O157:H7 LPF in intestinal colonization of lambs were investigated. We found that expression of lpf1 was regulated in response to growth phase, osmolarity, and pH; that lpf2 transcription was stimulated during late exponential growth and iron depletion; and that LPF impacts the ability of E. coli O157:H7 to persist in the intestine of infected 6-week-old lambs.

Mapping evidence gaps in anti-corruption: assessing the state of the operationally relevant evidence on donors' actions and approaches to reducing corruption

This paper charts the current evidence on effectiveness of different anti-corruption reforms, and identifies significant evidence gaps. Despite a substantial amount of literature on corruption, this review found very few studies focusing on anti-corruption reforms, and even fewer that credibly assess issues of effectiveness and impact. The evidence was strong for only two types of interventions: public financial management (PFM) reforms and supreme audit institutions (SAIs). For PFM, the evidence in general showed positive results, whereas the effectiveness was mixed for SAIs. No strong evidence indicates that any of the interventions pursued have been ineffective, but there is fair evidence that anti-corruption authorities, civil service reforms and the use of corruption conditionality in aid allocation decisions in general have not been effective. The paper advocates more operationally-relevant research and rigorous evaluations to build up the missing evidence base, particularly in conflict-afflicted states, in regards to the private sector, and on the interactions and interdependencies between different anti-corruption interventions.

Final warming of the Southern Hemisphere polar vortex in high- and low-top CMIP5 models

The final warming date of the polar vortex is a key component of Southern Hemisphere stratospheric and tropospheric variability in spring and summer. We examine the effect of external forcings on Southern Hemisphere final warming date, and the sensitivity of any projected changes to model representation of the stratosphere. Final warming date is calculated using a temperature-based diagnostic for ensembles of high- and low-top CMIP5 models, under the CMIP5 historical, RCP4.5, and RCP8.5 forcing scenarios. The final warming date in the models is generally too late in comparison with those from reanalyses: around two weeks too late in the low-top ensemble, and around one week too late in the high-top ensemble. Ensemble Empirical Mode Decomposition (EEMD) is used to analyse past and future change in final warming date. Both the low- and high-top ensemble show characteristic behaviour expected in response to changes in greenhouse gas and stratospheric ozone concentrations. In both ensembles, under both scenarios, an increase in final warming date is seen between 1850 and 2100, with the latest dates occurring in the early twenty-first century, associated with the minimum in stratospheric ozone concentrations in this period. However, this response is more pronounced in the high-top ensemble. The high-top models show a delay in final warming date in RCP8.5 that is not produced by the low-top models, which are shown to be less responsive to greenhouse gas forcing. This suggests that it may be necessary to use stratosphere resolving models to accurately predict Southern Hemisphere surface climate change.

The boreal forest and the polar front

The analysis presented in this paper suggests that the larger heating over the boreal forest in the spring and summer, as contrasted with weaker heating over the adjacent tundra, results in a preferred position of the polar front along the northern edge of the boreal forest. This positioning is well documented in the literature (see, for example, Bryson, 1966; Barry and Hare, 1974; Kreps and Barry, 1970). This heating results from the lower albedo of the boreal forest which is not compensated by an increase in transpiration, even with the larger leaf area index of the forest. The warmer temperatures are mixed upward by the deep boundary layer over the forest and mesoscale circulations which result from the patchiness of heating associated with the heterogeneous landscapes of the forest. Thus in contrast to previous assumptions in which the arctic front position in the summer determines the northern limit of the boreal tree line, our study suggests the boreal forest itself significantly influences the preferred position of the front. This conclusion reinforces the findings of Bonan et al. (1992) and Foley et al. (1994) on the important role of boreal forest-tundra interactions with climate.

Electrical signature in polar night cloud base variations

Layer clouds are globally extensive. Their lower edges are charged negatively by the fair weather atmospheric electricity current flowing vertically through them. Using polar winter surface meteorological data from Sodankyla ̈ (Finland) and Halley (Antarctica), we find that when meteorological diurnal variations are weak, an appreciable diurnal cycle, on average, persists in the cloud base heights, detected using a laser ceilometer. The diurnal cloud base heights from both sites correlate more closely with the Carnegie curve of global atmospheric electricity than with local meteorological measurements. The cloud base sensitivities are indistinguishable between the northern and southern hemispheres, averaging a (4.0 ± 0.5) m rise for a 1% change in the fair weather electric current density. This suggests that the global fair weather current, which is affected by space weather, cosmic rays and the El Nin ̃o Southern Oscillation, is linked with layer cloud properties.