Effect of L-Prolyl-L-Leucyl-Glycinamide (PLG) on Neuroleptic-Induced Catalepsy and Dopamine/Neuroleptic Receptor Bindings

Effect of L-prolyl-cinagta tlheep spyo atenndt idaol paanmti-iPnaer/nkeinusroonleiapnti cp rreocpeeprttoiers b oifn dLi-npgrso.lyPl E-LP-TleIuDcEylS- g2l(y1c)Li n1-a0lem5u-ic1dy1el1-g,(Ply1Lc9iG8n1)a. mw-Taidhsee i nm(vPeeLcstGhiag) anotinesmd n ie onuf rb oaelchetapiovtinico -suuirnbadslu eacrnevddetnarflefetueeacrrtmto a coephfnp ePtrmLe(2icGc0iaa, lob4 mnl0y io atndnhedevl sii8tn r0oto fem dndgosoi ppktyaag mm o-1fii nn tSeehCr/eng cteiwcau tfiracuolenle edpcptattiiioiclcny r r feienoscrp et ohfpinetvos erer ad ebtali.iyncAsdit)cienusdgit ge bin nyai dfrhimacaatli nonsttpilrseytirar aiatdtuttoimeolnn u(a3aso tmfde PidgfL f hkeGargel -o(n'p2tI0ieaPr ali)ldn.y odB ll ay4-b 0icne omldlneugtdc rk eabgdsy t - c1,aa pcSthoaCrmleo)ponfrsaicypil .heP TidLn hteGoe pahnidn esp tior odpoepraimdoinl ew raesc aelpstoo ersx ainm tihnee dst.rPiaLtuGm s,elbeuctt ihvaedly n eon ehfafneccte don t h['eH a]ffsipniirtoyp oefr tidhoel sbpiencdifinicg .b Tinhdei nbge hoafv aigouonraislt an[3dH b] iaopcohmemori-- cal results obtained in the present study raise the possibility that PLG may facilitate nigro-striatal dopaminergic neurotransmission through interacting with a unique PLG receptor functionally coupled to the dopamine receptor cyclase complex. -adenylate

Alkaline protease from a non-toxigenic mangrove isolate of Vibrio sp. V26 with potential application in animal cell culture

Vibrio sp. V26 isolated from mangrove sediment showed 98 % similarity to 16S rRNA gene of Vibrio cholerae, V. mimicus, V. albensis and uncultured clones of Vibrio. Phenotypically also it resembled both V. cholerae and V. mimicus.Serogrouping, virulence associated gene profiling, hydrophobicity, and adherence pattern clearly pointed towards the non—toxigenic nature of Vibrio sp. V26. Purification and characterization of the enzyme revealed that it was moderately thermoactive, nonhemagglutinating alkaline metalloprotease with a molecular mass of 32 kDa. The application of alkaline protease from Vibrio sp. V26 (APV26) in sub culturing cell lines (HEp-2, HeLa and RTG-2) and dissociation of animal tissue (chick embryo) for primary cell culture were investigated. The time required for dissociation of cells as well as the viable cell yield obtained by while administeringAPV26 and trypsin were compared. Investigations revealed that the alkaline protease of Vibrio sp. V26 has the potential to be used in animal cell culture for subculturing cell lines and dissociation of animal tissue for the development of primary cell cultures, which has not been reported earlier among metalloproteases of Vibrios.