Studies on the female reproductive physiology of the prawn, Macrobrachium idella (Hilgendorf, 1898)

’l‘he reproductive physiology of the female palaemonid prawn M. idella has been investigated by adopting a comprehensive approach to the problem. The major aspects of the study included investigations on breeding biology and process of oogenesis, variations in the biochemical components in relation to maturation, neuroendocrine relations and control over reproduction, and artificial insemination. The prawns used in the present study were procured from Vembanad Lake at Panavally village - a place nearly 20 km. away from Cochin. The studies were carried out using standard histological and biochemical methods. The modern technique of electroejaculation was adopted for extrusion of spermatophores in artificial insemination experiment.

Studies on the reproductive physiology and breeding biology of the cultivable species of grouper Epinephelus tauvina (Forsskal)

School of Industrial Fisheries,Cochin University of Science and Technology

Some studies on the reproductive endocrinology of tiger prawn Penaeus monodon Fabricius

The growing demand for quality prawn seed from the farmers‘ and entrepreneurs, coupled with uncertainity of their availability from nature at the appropriate time in required quantities has prompted‘ research on problems connected with prawn seed production. Endocrine control of reproduction in the penaeid shrimp _P_. monodon has been investigated in detail by adopting a comprehensive approach to the problem. The major aspects of the study included in depth investigations of the cytological details of the reproductive and neuroendocrine organs in correlation with the process of gonadal maturation. Based on the conclusions drawn from such ultrastructural studies various endocrine manipulations were carried out to see their effects on gonadal maturation. Besides that studies on the reproductive quality of male P_. monodon and the cryopreservation of spermatophores form a part of the present investigation. The shrimp 3; Inonodon used in the present study were collected from the offshore waters of Cochin, Madras and Mandapam and from the culture ponds of Vypeen Island near Cochin (Kerala) . The entire investigation on the cytological aspects were carried out using standard histological and electron-microscopic methods. Endocrine manipulations and cryopreservation studies is also carried out using the standard methods.

Studies on some aspects of the reproductive physiology of the female grey mullet mugil cephalus l.

Aquaculture is the dynamic pursuit of production of organisms from water a process analogous to agriculture on land. The field of aquaculture is an emerging bioindustry, based upon the culture and husbandry of economically utilizable aquatic organisms. Of late, there has been a global upsurge for aquaculture, the main reasons for which include the requirement of protein source for the increasing world population, the decision by various world nations to increase the fish yield by developing unutilized or partially utilized water bodies and depletion of natural stock which is evident in recent years due to excessive exploitation .The present study has been taken up on the reproductive physiology of the female grey mullet, M. cephalus. The thesis is presented in seven chapters. In the present study, variations in the major biochemical parameters namely, moisture, proteins, lipids, carbohydrates cholesterol, carotenoid, ash, calcium and iron in four tissues E. muscle, liver, ovary and bloodserum of cephalus have been analysed at different maturity stages.

STUDIES ON SOME ASPECTS OF REPRODUCTION OF FEMALE ANABAS TESTUDINEUS ( BLOCH )

Department of Marine Biology, Microbiology and Biochemistry

Studies on the haemolymph of penaeus indicus H. MILNE EDWARDS

The objective of the study isto determine the average quantity of certain biochemical constituents of the haemolymph of Penaeus indicus and to verify the importanceof the simple correlation between the quantity or content of the biochemical constituents in the haemolymph and the size of the species, sex, moult and reproductive stages. The biochemical constituents studied are protein, free amino acids, glucose, total lipids, cholesterol, calcium, zinc, iron and manganese. The study Identifies the species specific haemolymph protein pattern by electrophoresis and determines the qualitative variations of haemolymph proteins with respect to sex, size, moult and reproductive stages. Major protein components such as hemocyanin and female specific protein are determined with a view to understand their function. The thesis also identifies the circulating haemocytes with a view to understand their specific role in the various physiological functions of the species. The thesis is presented in three chapters. Each chapter has an introduction to the particular aspect of study which includes a review of literature, methodology adopted for the study, the results obtained and discussion on the subject. The first Chapter deals with the biochemical constituents of the haemolymph, the second includes electrophoretic characterization of proteins in the haemolymph and the third Chapter deals with haemocyte identification and classification. A summary of the thesis and literature cited in the text are listed at the end.

Studies on the reproductive physiology of the green tiger prawn Penaeus (penaeus) semisulcatus de Haan

A study of the reproductive physiology of P. (P). semisulcatus wasundertaken as this information is an essential prerequisite for brood stock development for hatchery operations, and the results are embodied in this thesis. The thesis is presented in seven chapters. The protein, lipid and carbohydrate contents of ovary were estimated for the different vitellogenic phases. The protein, lipid, carbohydrate and cholesterol contents were estimated in testes and spermatophore.The thesis present the results of induced maturation experiments by eyestalk ablation, CNS extract injection and UV rays application on immature female prawns.