Studies on the Survival of Sunetta Scripta in Different Salinities

Division of Marine Biology, Microbiology and Biochemistry, School of Marine Sciences, Cochin University of Science and Technology

Investigations on the Effects of Petroleum Hydrocarbons on the Marine Bivalves Perna viridis (Linnaeus) and Sunetta scripta (Linne')

The thesis deals with different aspects connected with the distribution and toxicity of PHC in crude oils in the aquatic environment and marine molluscs. Information has been gathered and presented on the present status of research in the field based on a thorough review of recent literature. It is seen from the literature that the resident time of PHC becomes lesser and lesser when crude oil is discharged in large quantities into the coastal waters where excess oxygenation light penetration, temperature availability and strong tidal currents persist. This results in the reduction of the onslaught of oil pollution. This probably shows that taxic insult of crude oil in tropical shores will be relatively lesser than that occurring in temperate and boreal waters. However, there is no record of major episodal oil pollution comparable in quantum and extent to that of Torrey Canyon or Amoco Cadiz which prevents a more reliable analysis on the issue. Considerable controversy exists on the method of estimation of PHC in seawater. However, Ultra-violet fluorescence spectroscopy has been widely recommended as an analytical tool for the determination of total hydrocarbons. Similarly representation of values as chrysene equivalents has been recognised as the most reliable method of Quantum representation.

Physiological effects of copper (II) on sunetta scripta L.

In India industrial pollution has become a subject of increasing concern.Incidents of industrial pollution have been reported from many parts of the country. Cochin, the collection site of the present study, being the industrial capital of Kerela is also a harbour, is vulnerable to pollution by trace metal contaminants. In the recent times, pollutants of greatest concern in the aquatic environment are those which are persistent such as toxic heavy metals and the chlorinated hydrocarbons which include insecticides and pesticides.The animals collected from the clam bed situated on the northern side af Cochin bermouth are subject to wide fluctuations in salinity both seasonal and tidal. also; salinity is considered as an important parameter influencing the.-physiological functioning of an organism. Hence, the salinity tolerance of the animal is worked out. Considering the potential vulnerability of Cochin backwaters to heavy metal pollution, the impact of heavy metal copper (II) on the bivalve Sunetta sripta was conceived. Static bioassays were conducted for the determination of the sublethal concentrations of the metal as a preliminary step towards the toxicity studies. Oxygen consumption and filtration rate which are considered as reliable sublethal toxicity indices were employed for investigating the toxic effects of the metal. Bioaccumulation, a physiological phenomenon which can be of importance from the public health point of view, and also in the assessment of environmental quality is also dealt with.

Studies On The Biology Of The Clam Sunetta Scripta (Linne), From The Subtidal Waters Of Cochin

Enhancement and culture of bivalves presents an opportunity to maximise and even increase production of many growing areas. Clam culture is less intensive both for capital and labour, involves simple farming and management techniques and is considered an efficient means of protein production. Clams are efficient converters of primary production and growth rate is fast with maximum production in 5-6 months. with culture, production is less influenced by poor recruitment. Stable production facilitates market development. Rivalves are being increasingly used in bio-medical research. Culture practices would ensure uninterrupted supplies of experimental material. Paucity of biological data restricts the development of efficient management and culture techniques of bivalves. This study was undertaken with a view to provide information on some aspects of biology of the bivalve S_. scripta which have hitherto been uninvestigated.

HYPOXIC ADAPTATIONS AND CAROTENOIDS OF TWO INTERTIDAL MOLLUSCS

The present investigation is to find the hypoxic adaptations and role of carotenoids in the anaerobic catabolism of two intertidal bivalves-Sunetta scripta and Perna viridis. Physiological and cytological responses during hypoxic stress have been studied and compared to that of sublethal heavy metal (copper) exposure using two indices : total carotenoid concentration and accumulation of lipofuscin granules. A close similarity has been observed between hypoxic exposed and copper (sublethal) exposed animals regarding the total carotenoid concentration and lipofuscin accumulation. In the case of S.scripta, the total caroteniod increase at 48h of both hypoxic and heavy metal exposure was found to be nearly 40% greater than that of the control (0h). Whereas in P.viridis, the increment in the total carotenoid concentration at 48h of hypoxic exposure and 48h of heavy metal exposure were found to be nearly 87% and 95% higher than that of the control (0h) respectively.Regarding the lipofuscin accumulation, in both S.scripta and P.viridis , the characteristic features of the granule at 48h of hypoxia is very much similar to that observed at 48h of heavy metal exposure. Thus, the present study suggests that the increase in carotenoid concentration and lipofuscin accumulation expressed by bivalves under heavy metal stress can be due to the indirect effect of hypoxia.

Studies on amylolytic bacteria in Cochin backwaters

This thesis Entitled Studies on amylolytic bacteria in cochin backwaters.This thesis presents a detailed account of the disribution of amylolytic bacteria in water. sediment. fishes ( Etroplus suratensis and Liza parsia) • prawns ( Penaeus indicus and Metapenaeus dobsoni) and clams ( Sunetta scripta and Meretrix casta) from Cochin backwaters. genera-wise distribution of amylolytic bacteria, ability of selected strains to grow and produce amylase at various physico-chemical conditions. Regulation of amylase synthesis anrt characters of amylases producer by these halophilic bacteria.Amylolytic bacteria are distributed widely in water. sediment. fishes. prawns and clams of Cochin back waters. 53% of the total isolates tested were capable of producing amylase. Maximum number of arnylolytic bacteria were present in Metapenaeus dobsoni. In general, the gut region of aquatic animals harboured more amylolytic bacteria than the gill or surface. These bacteria may help in the digestion of starch present in their food.Presence of ions in the medium was found to be essential for growth and amylase production. It was found that this ionic requirement is not highly specific. Sorlium chloride could be replaced by potassium chloride. or magnesium chloride to some extent I without affecting growth and amylase production. The important function of these ions may be to maintain the osmotic balance between the cells and their environment.All the isolates showed the ability to grow and produce amylase using raw-starches from cassava. plantain and potato .This property suggests their role in the rdegradation of native starches in the environment

Haematology of some marine and estuarine molluscs of commercial importance

It is well known that under certain conditions, populations of oysters and clams are susceptible to destructive epizootics caused by pathogenic micro-organisms. It has also been shown that exposure of mammals to certain heavy metals causes increased susceptibility to and severity of microbial infections (Koller, 1980). Consequently, pollutants that affect haemocyte viability or interfere with internal defence functions of the haemocytes which are considered as the major means of defence in moliuscs against invading foreign organisms and pathogens (Cheng, 1981) may have profound effect on long term survival of molluscan populations. All these justify the significance of the present study in the context of the current status on molluscan culture programme, and how the data on molluscan haematological studies .could be taken as the reliable criteria for pollution monitoring studies.