16 resultados para 270601 Animal Physiology - Biophysics
Filtro por publicador
- Repository Napier (1)
- ABACUS. Repositorio de Producción Científica - Universidad Europea (1)
- AMS Tesi di Dottorato - Alm@DL - Università di Bologna (1)
- Aquatic Commons (24)
- Archivo Digital para la Docencia y la Investigación - Repositorio Institucional de la Universidad del País Vasco (8)
- Aston University Research Archive (3)
- Biblioteca Digital da Câmara dos Deputados (1)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (5)
- Biblioteca Digital da Produção Intelectual da Universidade de São Paulo (BDPI/USP) (2)
- Biblioteca Digital de Teses e Dissertações Eletrônicas da UERJ (3)
- Biodiversity Heritage Library, United States (3)
- BORIS: Bern Open Repository and Information System - Berna - Suiça (18)
- Brock University, Canada (12)
- CaltechTHESIS (3)
- Cámara de Comercio de Bogotá, Colombia (1)
- Cambridge University Engineering Department Publications Database (1)
- CentAUR: Central Archive University of Reading - UK (3)
- Chinese Academy of Sciences Institutional Repositories Grid Portal (41)
- Cochin University of Science & Technology (CUSAT), India (16)
- CORA - Cork Open Research Archive - University College Cork - Ireland (3)
- Deakin Research Online - Australia (5)
- DI-fusion - The institutional repository of Université Libre de Bruxelles (4)
- Digital Commons at Florida International University (6)
- DigitalCommons@The Texas Medical Center (87)
- DRUM (Digital Repository at the University of Maryland) (2)
- Duke University (10)
- eResearch Archive - Queensland Department of Agriculture; Fisheries and Forestry (34)
- FAUBA DIGITAL: Repositorio institucional científico y académico de la Facultad de Agronomia de la Universidad de Buenos Aires (5)
- Gallica, Bibliotheque Numerique - Bibliothèque nationale de France (French National Library) (BnF), France (7)
- Harvard University (1)
- Helda - Digital Repository of University of Helsinki (14)
- Indian Institute of Science - Bangalore - Índia (43)
- Infoteca EMBRAPA (10)
- Instituto Politécnico do Porto, Portugal (2)
- Massachusetts Institute of Technology (1)
- Ministerio de Cultura, Spain (1)
- National Center for Biotechnology Information - NCBI (1)
- Plymouth Marine Science Electronic Archive (PlyMSEA) (39)
- Portal de Revistas Científicas Complutenses - Espanha (2)
- Publishing Network for Geoscientific & Environmental Data (2)
- QSpace: Queen's University - Canada (2)
- QUB Research Portal - Research Directory and Institutional Repository for Queen's University Belfast (188)
- Queensland University of Technology - ePrints Archive (106)
- Repositório Científico da Universidade de Évora - Portugal (6)
- Repositório Científico do Instituto Politécnico de Lisboa - Portugal (2)
- Repositório do Centro Hospitalar de Lisboa Central, EPE - Centro Hospitalar de Lisboa Central, EPE, Portugal (2)
- Repositório Institucional da Universidade de Aveiro - Portugal (3)
- Repositorio Institucional de la Universidad Nacional Agraria (14)
- Repositório Institucional UNESP - Universidade Estadual Paulista "Julio de Mesquita Filho" (25)
- RUN (Repositório da Universidade Nova de Lisboa) - FCT (Faculdade de Cienecias e Technologia), Universidade Nova de Lisboa (UNL), Portugal (3)
- SAPIENTIA - Universidade do Algarve - Portugal (2)
- School of Medicine, Washington University, United States (2)
- Universidad Autónoma de Nuevo León, Mexico (8)
- Universidad del Rosario, Colombia (2)
- Universidad Politécnica de Madrid (2)
- Universidade de Lisboa - Repositório Aberto (2)
- Universidade Estadual Paulista "Júlio de Mesquita Filho" (UNESP) (2)
- Universitat de Girona, Spain (1)
- Universitätsbibliothek Kassel, Universität Kassel, Germany (3)
- Université de Lausanne, Switzerland (26)
- Université de Montréal (4)
- Université de Montréal, Canada (59)
- University of Michigan (25)
- University of Queensland eSpace - Australia (71)
- University of Southampton, United Kingdom (3)
- University of Washington (1)
- WestminsterResearch - UK (2)
Resumo:
Vibrio sp. V26 isolated from mangrove sediment showed 98 % similarity to 16S rRNA gene of Vibrio cholerae, V. mimicus, V. albensis and uncultured clones of Vibrio. Phenotypically also it resembled both V. cholerae and V. mimicus.Serogrouping, virulence associated gene profiling, hydrophobicity, and adherence pattern clearly pointed towards the non—toxigenic nature of Vibrio sp. V26. Purification and characterization of the enzyme revealed that it was moderately thermoactive, nonhemagglutinating alkaline metalloprotease with a molecular mass of 32 kDa. The application of alkaline protease from Vibrio sp. V26 (APV26) in sub culturing cell lines (HEp-2, HeLa and RTG-2) and dissociation of animal tissue (chick embryo) for primary cell culture were investigated. The time required for dissociation of cells as well as the viable cell yield obtained by while administeringAPV26 and trypsin were compared. Investigations revealed that the alkaline protease of Vibrio sp. V26 has the potential to be used in animal cell culture for subculturing cell lines and dissociation of animal tissue for the development of primary cell cultures, which has not been reported earlier among metalloproteases of Vibrios.