In vivo effect of deoxynivalenol (DON) naturally contaminated feed on porcine reproductive and respiratory syndrome virus (PRRSV) infection.

Deoxynivalenol (DON), also known as vomitoxin, is the most prevalent type B trichothecene mycotoxin worldwide. Pigs show a great sensitivity to DON, and because of the high proportion of grains in their diets, they are frequently exposed to this mycotoxin. The objective of this study was to determine the impact of DON naturally contaminated feed on porcine reproductive and respiratory syndrome virus (PRRSV) infection, the most important porcine viral pathogen in swine. Experimental infections were performed with 30 animals. Piglets were randomly divided into three groups of 10 animals based on DON content of diets (0, 2.5 and 3.5 mg/kg DON). All experimental groups were further divided into subgroups of 6 pigs and were inoculated with PRRSV. The remaining pigs (control) were sham-inoculated with PBS. Pigs were daily monitored for temperature, weight and clinical signs for 21 days. Blood samples were collected and tested for PRRSV RNA and for virus specific antibodies. Results of PRRSV infection showed that ingestion of diet highly contaminated with DON greatly increases the effect of PRRSV infection on weight gain, lung lesions and mortality, without increasing significantly viral replication, for which the tendency is rather directed toward a decrease of replication. These results suggest that PRRSV infection could exacerbate anorectic effect of DON, when ingested in large doses. Results also demonstrate a DON negative effect on PRRSV-specific humoral responses. This study demonstrate that high concentrations of DON naturally contaminated feed decreased the immune response against PRRSV and influenced the course of PRRSV infection in pigs.

Between pragmatism and the defence of a “Sister State” : the national association for the advancement of colored people and the U.S. occupation of Haiti, 1915-1922

À l’origine, la nouvelle concernant l’occupation américaine d’Haïti en 1915 a suscité peu d’indignation aux États-Unis. En effet, on reproche à la république son instabilité politique et on juge aussi qu’une intervention américaine concourrait à l’édifice de l’autorité de la loi. À partir de 1915 et surtout en 1920, l’Association nationale pour l’avancement des gens de couleur (NAACP), fondée en 1909, critique cette ingérence et milite pour y voir un terme. W.E. B. Du Bois et James Weldon Johnson, deux figures publiques noires importantes travaillant au sein de l’organisation, dénoncent avec conviction l’occupation d’Haïti. Les historiens ont jusqu’ici jugé que la NAACP fut inspirée par des considérations de solidarité raciale en adhérant à la cause de la souveraineté haïtienne. Si la thèse présente ne réfute pas cette possibilité, elle cherche tout de même à démontrer que le cadre conceptuel de la solidarité raciale ne saurait illustrer toute la complexité de la campagne haïtienne érigée par la NAACP. Par conséquent, une attention dirigée davantage sur le contexte social et politique américain entre 1915 et 1922 révèle que pour la NAACP, la dénonciation de l’occupation américaine d’Haïti représentait d’une part une opportunité de discuter des problèmes sociaux touchant les Afro-Américains, et d’autre part, une occasion de renforcer sa position aux États-Unis.

Deoxynivalenol (DON) naturally contaminated feed impairs the immune response induced by porcine reproductive and respiratory syndrome virus (PRRSV) live attenuated vaccine

Cereal commodities are frequently contaminated with mycotoxins produced by the secondary metabolism of fungal infection. Among these contaminants, deoxynivalenol (DON), also known as vomitoxin, is the most prevalent type B trichothecene mycotoxin worldwide. Pigs are very sensitive to the toxic effects of DON and are frequently exposed to naturally contaminated feed. Recently, DON naturally contaminated feed has been shown to decrease porcine reproductive and respiratory syndrome virus (PRRSV) specific antibody responses following experimental infection. The objective of this study was to determine the impact of DON naturally contaminated feed on the immune response generated following vaccination with PRRSV live attenuated vaccine. Eighteen pigs were randomly divided into three experimental groups of 6 animals based on DON content of the diets (0, 2.5 and 3.5 mg DON/kg). They were fed these rations one week prior to the vaccination and for all the duration of the immune response evaluation. All pigs were vaccinated intra-muscularly with one dose of Ingelvac® PRRSV modified live vaccine (MLV). Blood samples were collected at day −1, 6, 13, 20, 27 and 35 post vaccination (pv) and tested for PRRSV RNA by RT-qPCR and for virus specific antibodies by ELISA. Results showed that ingestion of DON-contaminated diets significantly decreased PRRSV viremia. All pigs fed control diet were viremic while only 1 (17%) and 3 (50%) out of 6 pigs were viremic in the groups receiving 3.5 and 2.5 mg of DON/kg, respectively. Subsequently, all pigs fed control diet developed PRRSV specific antibodies while only viremic pigs that were fed contaminated diets have developed PRRSV specific antibodies. These results suggest that feeding pigs with DON-contaminated diet could inhibit vaccination efficiency of PRRSV MLV by severely impairing viral replication.