The identification and characterization of inter- and intra-species genetic diversity derived from retrotransposons in humans

Retrotransposons, which used to be considered as “junk DNA”, have begun to reveal their immense value to genome evolution and human biology due to recent studies. They consist of at least ~45% of the human genome and are more or less the same in other mammalian genomes. Retrotransposon elements (REs) are known to affect the human genome through many different mechanisms, such as generating insertion mutations, genomic instability, and alteration in gene expression. Previous studies have suggested several RE subfamilies, such as Alu, L1, SVA and LTR, are currently active in the human genome, and they are an important source of genetic diversity between human and other primates, as well as among humans. Although several groups had used Retrotransposon Insertion Polymorphisms (RIPs) as markers in studying primate evolutionary history, no study specifically focused on identifying Human-Specific Retrotransposon Element (HS-RE) and their roles in human genome evolution. In this study, by computationally comparing the human genome to 4 primate genomes, we identified a total of 18,860 HS-REs, among which are 11,664 Alus, 4,887 L1s, 1,526 SVAs and 783 LTRs (222 full length entries), representing the largest and most comprehensive list of HS-REs generated to date. Together, these HS-REs contributed a total of 14.2Mb sequence increase from the inserted REs and Target Site Duplications (TSDs), 71.6Kb increase from transductions, and 268.2 Kb sequence deletion of from insertion-mediated deletion, leading to a net increase of ~14 Mb sequences to the human genome. Furthermore, we observed for the first time that Y chromosome might be a hot target for new retrotransposon insertions in general and particularly for LTRs. The data also allowed for the first time the survey of frequency of TE insertions inside other TEs in comparison with TE insertion into none-TE regions. In summary, our data suggest that retrotransposon elements have played a significant role in the evolution of Homo sapiens.

Marriage Certificate - Charles Nickles & Margaret Nelles

A marriage license for "Charles Nickles of the Township of Grimsby Gentleman and Margaret Nelles of Same place Spinster" and is dated 23 July, 1831.

Marriage Certificate - Thomas Cowan & Annie Easling, 1884

A marriage certificate for Thomas Cowan of Thorold and Annie Easling of St. Catharines. The certificate is dated October 8, 1884 and is witnessed by W. Ross and Jennie McElroy.

Invitation: 61st Inter-Parliamentary Conference Tokyo 1974 Inaugural Ceremony

An invitation to the Inaugural Ceremony of the 61st Inter-Parliamentary Conference in Japan October 1974. The invitation reads: "The Japanese Group of the Inter-Parliamentary Union has the honour of inviting you to attend the Inaugural Ceremony of the 61st Inter-Parliamentary Conference to be held in the Chamber of the House of Councillors on Wednesday the 2nd of October 1974, at 3 p.m. The Ceremony will be honoured by the presence of Their Majesties the Emperor and Empress of Japan".

Marriage Certificate - Joseph Clement and Margaret Duffet

A marriage certificate stating that Joseph Clement and Margaret Duffet were lawfully married according to the Church of England in Montreal, May 9, 1784. The name on the paper appears to be "Margaret Dusalle" and the date appears as "May 7th".

Marriage Certificate - Hamilton K. Woodruff and Julia C. Cleveland

The marriage certificate of Mr. Hamilton K. Woodruff of St. Catharines and Miss Julia C. Cleveland of Erie, Pennsylvania. This is certificate no. 5221 signed by James C. Wilson. This is accompanied by 2 envelopes; one envelope is from Henry L. Rea, clerk of courts, Erie Pennsylvania to Mr. H. K. Woodruff, the other envelope just says "marriage certificate Mrs. H. K. Woodruff", Nov. 21, 1894.

Marriage Certificate - Percy Carruthers Band and Margaret Julia Woodruff

A marriage certificate of Percy Carruthers Band of the city of New York, U.S.A. and Margaret Julia Woodruff of St. Catharines, Ontario, November 25, 1919. The witnesses are C.S. Band and M.S. Walker. The marriage is registered in St. George's Church.

Marriage Certificate Card - Percy Carruthers Band and Margaret Julia Woodruff

A marriage certificate card stating that Percy Carruthers Band and Margaret Julia Woodruff were married in St. Catharines with envelope which has the return address: James Carruthers and Company, Ltd., Grain Exporters, Produce Exchange Building, New York, November 25, 1919.

Clippings - Births, Deaths, Marriage

Clipping of the obituary of Mrs. Mary Lanman Douw Ferris, widow of Morris Patterson Ferris, n.d. Clipping of the obituary of Alfred Sanderson Woodruff, March 20, 1926. Clipping describing the wedding of Margaret Julia Woodruff and Captain Percy Carruthers Band, n.d. Clipping of a birth announcement of a daughter to Mr. and Mrs. P.C. Band, 1924. Clipping of a birth announcement of a son to Mr. and Mrs. Percy C. Band, Sept. 11, 1927.

Letter regarding a special service held by DeVeaux School to re-inter the body of Mrs. Maria Woodruff

Letter (2 page typed form letter with a handwritten note from Don Loker) regarding a special service held by DeVeaux School to re-inter the body of Mrs. Maria Woodruff, the first wife of Judge Samuel DeVeaux. Her body was originally buried at DeVeaux School in Niagara Falls, New York. The location of the ceremony was the cemetery in St. Davids, Ontario. Maria died on April 23, 1815 at the age of 19. This notice was sent by Rev. Alec Pudwell, chaplain of DeVeaux School, Niagara Falls, New York, May 6, 1963.

Marriage Bond between Harrison Narrel of Gainsborough and Anna Simmermon of Clinton

Marriage Bond between Harrison Narrel of Gainsborough and Anna Simmermon of Clinton signed by Andrew Snyder of Gainsborough and William Jacobs of Grimsby, Sept. 1, 1834

Marriage Bond between Henry Chambers of Ancaster and Sarah Smith of Ancaster

Marriage Bond between Henry Chambers of Ancaster and Sarah Smith of Ancaster signed by Charles Anderson of Grimsby and Robert F. Nelles of Grimsby, Dec. 27, 1836.

Marriage Bond between John Smith of Glanford in the Gore district and Jane Atkins of Ancaster

Marriage Bond between John Smith of Glanford in the Gore district and Jane Atkins of Ancaster signed by Edmund Smith of Ancaster and Jeremiah Smith of Glanford, Jan. 17, 1837.

Marriage Bond between Joseph Bridgeman of Grimsby and Calinda Ann Lounsberry of Grimsby

Marriage Bond between Joseph Bridgeman of Grimsby and Calinda Ann Lounsberry of Grimsby signed by Palmer Buckbee of Grimsby and Robert F. Nelles of Grimsby, July [?] 6, 1837.