Skeletal muscle fibre-type comparison of whole tissue and subcellular membrane phospholipids and fatty acids

Membranes are dynamic structures that affect cell structure and function. Compositional changes ofmembranes have been shown with the application of a perturbation; however these are limited to whole tissue analysis. The purpose of this thesis was to compare the phospholipid (PL) fatty acid (FA) composition of rat whole muscle (Wm) to 1) purified and non-purified subsarcolemmal (SS) mitochondria in soleus, plantaris, and red gastrocnemius, and 2) sarcolemma, transverse-tubules, SS and intermyofibrillar (IMF) mitochondria fix)m whole hindlimb. The major findings were that 1) contamination significantly altered the PL FA composition of the SS mitochondrial membrane fraction, 2) Wm and SS mitochondria compositions differed between muscle types, and 3) Wm did not accurately reflect the PL FA composition of any isolated subcellular membranes, with each being unique from each other. As such, the relevancy of the trends reported in the literature of the effects of perturbations on Wm may be limited.

American and Luna Falls, and River, from Goat Island, Niagara, U.S.A.

The image is described as "(9) Marble whiteness of the seething Waters-American and Luna Falls, and River from Goat Island, Niagara, U.S.A."

Rapids Above the Falls Looking at Goat Island, Niagara, U.S.A.

The description reads "(14) The rolling, tumbling "Rapids" above the Falls - along Riverside Drive, looking toward Goat Island, - Niagara, U.S.A.".

American Falls, Niagara From Goat Island

The description below simply states "116 American Falls, Niagara From Goat Island".

Cave of the Winds, Goat Island, U.S.A.

Cave of the Winds, Goat Island, U.S.A. This is touted as the only place on the globe where rainbows form an entire circle. Geo. W. Wright is listed as the lessee. 30 cm. x 14 cm. newsprint. There is a slight wearing away of the edges. This does not affect text, n.d.