Host-parasite relations in a mycoparasitic system : alterations in membrane permeability and lipid composition in Choanephora cucurbitarum infected by Piptocephalis virginiana /

examined in Choanephora cucurbita rum during the early stages of infection by Piptocephalis virginiana » There was a small but consistent increase in the leakage of electrolytes, amino acids and sugars as a result of infection. These low levels of differential leakage in infected tissues are explained on the basis of the nature of this obligate, biotrophic, mycoparasitic system. Quantitative analysis of the twenty six amino acids and amino compounds detected in the leacheates — showed similar profiles in infected and control host and no new species of amino acids or amino compounds were detected in either infected or control host leacheates. Comparatively high amounts of aspartic acid, glutamic acid and alanine were found in the leacheates of host and infected host . Analyses of the sugars comprising the leacheates of infected and control host showed the presence of eight sugars, among which glucose was found in significant amounts (50-53%) ' The nutritional implication of this preferential leakage is discussed. No significant difference was observed in the leacheates of infected host sugar profiles compared with that of the control host. Profiles of the internal pool sugars of infected and control host did not reflect that obtained from the leacheate data, perhaps owing to leakage of sugars in a selective manner . Membrane lipid analyses yielded higher levels of lipid in infected host compared with the control, both at the 24 h and 36 h analyses. In addition, preliminary investigations of phosphorous-32 incorporation and turnover in phospholipids showed higher levels of 32p incorporation and turnover in infected host compared with the control. No apparent difference was noted in the profiles of the neutral lipid classes and the polar lipid classes of the membrane lipids as determined by one and two dimensional thin-layer chromatography respectively. However, a small but consistently higher degree of unsaturation was detected in the fatty acids of infected tissue compared with the control. Also, '^''-^^''^^'-'-^'^^c acid, a polyunsaturated fatty acid previously reported to show a direct correlation during the early stages of infection and the degree of parasitism of P. virginiana on C. cucurbitarum , was found in higher amounts in infected host membrane lipids compared with that of the control host. The implications of these membrane lipid alterations are discussed with particular reference to the small but consistently higher leakage of electrolytes, amino acids and sugars observed during infection in this study.

Receipt from Rolph Smith Lithographers by Steam Power and Co. of Toronto

Receipt from Rolph Smith Lithographers by Steam Power and Co. of Toronto for a name plate, Aug. 23, 1886.

Receipt from John Burrow, Steam and Gas Fitter and Plumber

Receipt from John Burrow, Steam and Gas Fitter and Plumber, St. Catharines for work done, March 31, 1887.

Flyer for the Georgian Bay, Ontario Steam Show, 1983

Flyer for the Georgian Bay, Ontario Steam Show, 1983.

Broadside with a coloured picture of a case engine and tender [steam engine]

Broadside, 47 cm. x 31 cm. with a coloured picture of a case engine and tender [steam engine] printed by Meyer-Rotier of Milwaukee. On the back is a sketch of a building. This has a stamp on the back which indicates that this is an exhibit in the High Court of Justice in Coburg in the case of Bigelow vs. Powers et al. This item has been torn down the middle and taped. This does not affect the text nor picture, Oct. 11, 1909.