Radiation-induced changes in the export of photoassimilated carbon /|nBarry Jess Shelp. -- 260 0 St. Catharines, [Ont. : s. n.],

Low levels of ionizing radiation induce two translocation responses in soybean: a reduction in photoassimilate export from leaves and a change in the distribution pattern of exported photoassimilate within the plant. In this investigation these responses have been further studied specifically to ascertain the site of radiation damage and to better understand the physiological responses observed. Experimentally the primary data was obtained from studies in which a mature trifoliate leaf of a young soybean plant (Glycine ~ L. cultivar Harosoy '63) is isolated in a closed transparent chamber and allowed to photoassimilate 14C02 for 15 minutes. This is followed by an additional 45 ~_il'1;ute period before the plant is sectl.o ne d an d 14 C-ra dl' oactl.v.l ty d eterml. ne d'l n a 11 parts. Such 14c data provides one with the magnitude and distribution pattern of translocation. Further analyses were conducted to determine the relative levels of the major photosynthetic products using the techniques of paper chromatography and autoradiography. Since differences between control and irradiated P 1 ants were not 0 b serve d l' n t h e par tl't"lo nlng 0 f 14 C between the 80% ethanol-soluble and -insoluble fractions 14 or in the relative amounts of C-products of photosynthesis, the reduction in export in irradiated plants is not likely due to reduced availability of translocatable materials. Data presented in this thesis shows that photoassimilate export was not affected by gamma radiation until a threshold dose between 2.0 and 3.0 krads was reached. It was also observed that radiation-induced damage to the export process was capable of recovery in a period of 1 to 2 hours provided high light intensity was supplied. In contrast, the distribution pattern was shown to be extremely radiosensitive with a low threshold dose between .25 and .49 krads. Although this process was also capable of recovery,lt" occurred much earlier and was followed by a secondary effect which lasted at least for the duration of the experiments. The data presented in this thesis is interpreted to suggest that the sites of radiation action for the two translocation responses are different. In regards to photoassimilate export, the site of action of ionizing radiation is the leaf, quite possibly the process of photophosphorylation which may provide energy directly for phloem loading and for membrane integrity of the phloem tissue* In regards to the pattern of distribution of exported photoassimilate, the site is likely the apical sink, possibly the result of changes of levels of endogenous hormones. By the selection of radiation exposure dose and time post-irradiation, it is possible to affect independently these two processes suggesting that each may be regulated independent of the other and involves a distinct site.

Sale of Land for Taxes to W.H. Dickson

Notice of sale of land for taxes to W. H. Dickson. This is a handwritten document. The land consists of 4 acres of Lot no. 12 in the 5th Concession of Wainfleet. This is dated May 23, 1857 [signatures are illegible]. On the back there is a note that Walter Hamilton Dickson has transferred this land to Joseph Augustus Woodruff, Dec. 3, 1861.

Indenture of transfer of parcels of land purchased at sales of land for taxes to Joseph A. Woodruff of the Town of Clifton

Indenture of transfer of parcels of land purchased at sales of land for taxes to Joseph A. Woodruff of the Town of Clifton. Transferred by The Honourable Walter Hamilton Dickson of the Town of Niagara. These lands are located in Caistor, Wainfleet, Humberstone, Crowland, Grimsby, Gainsboro and Pelham, Nov. 1, 1861.

Indenture of deed for taxes between Benjamin Walker Smith, sheriff of the County of Simcoe and Joseph A. Woodruff of the Town of Clifton

Indenture of deed for taxes between Benjamin Walker Smith, sheriff of the County of Simcoe and Joseph A. Woodruff of the Town of Clifton for 98 acres in the Township of Tiny in the County of Simcoe, Lot no. 15 in the 18th Concession, Dec. 12, 1861.

List (1 page, handwritten) of taxes paid for lands on the Sarnia Branch

List (1 page, handwritten) of taxes paid for lands on the Sarnia Branch, n.d.

List entitled “Balance acct.” including taxes, trips to St. Catharines and produce

List entitled “Balance acct.” including taxes, trips to St. Catharines and produce, 1870.

Receipt for taxes paid in Merritton

Receipt for taxes paid in Merritton, Sept. 30, 1886.

Receipt for personal taxes signed by W. M. Gibbon

Receipt for personal taxes signed by W. M. Gibbon, collector, July 20, 1887.

Receipt from the City of St. Catharines, Ward of St. Thomas for taxes,

Receipt from the City of St. Catharines, Ward of St. Thomas for taxes, Aug. 8, 1887.

Receipt from the City of St. Catharines for taxes

Receipt from the City of St. Catharines for taxes, Aug. 8, 1887.

Receipt from Merritton for taxes

Receipt from Merritton for taxes, Sept. 15, 1887.

Receipt from the City of St. Catharines for taxes

Receipt from the City of St. Catharines for taxes, Sept. 23, 1887.