Amino acid transport : the special case of a H/L-glutamate cotransport system in Asparagus sprengeri mesophyll cells /

The addition of L-Glutamate (L-GLU) and L-Hethionine ~ulfoximine (L-HSO) to mechanically isolated. photosynthetically competent, Asparagus sprengeri mesophyll cells ~u~pended in 1mM CaS04 cau~ed an immediate transient alkalinization of the cell su~pension medium in both the light and dark. The alkalinization response was specific and stereospecific as none of the L-isomers of the other 19 protein amino acids tested or D-GLU gave this response. Uptake of 14C-L-GLU was stimulated by the light. The addition of non-radioactive L-GLU. or L-GLU analogs together with 14C-L-GLU showed that only L-GLU and L-HSO stimulated alkalinization whilst inhibiting the uptake of 14C-L-GLU. Both the L-GLU dependent alkalinization and the upt~ke of 14C-L-GLU were stimulated when the external pH was decreased from 6.5 to 5.5. Increasing external K+ concentrations inhibited the uptake of 14C-L-GLU. Fusicoccin (FC) stimulated uptake. The L-GLU dependent alkalinization re~ponse exhibited monophasic saturation kinetics while the uptake of 14C-L-GLU exhibited biphasic saturation kinetics. In addition to a saturable component. the uptake kinetics also showed a linear component of uptake. Addition of L-GLU and L-MSO caused internal acidification of the cell as measured by a change in the distribution of 14C-DMO. There was no change in K+ efflux when L-GLU was added. A H+ to L-GLUinflux stoichiometry of 3:1 wa~ mea~ured at an external I.-GLU concentration of O.5mM and increased with increasing external 13 L-QLU concentration. Metabolism of L-GLU was detected manometrlcally by observing an increase in COa evolution upon the addition of L-QLU and by detection of i*C02 evolution upon the addition of »*C-L-GLU. »*C02 evolution was higher in the dark than in the light. The data are consistent with the operation of a H+/L-QLO cotransport system. The data also show that attempts to quantify the stoichlometry of the process were complicated by the metabolism of L-GLU.

Brock University Retirees Association fonds, 1997-2005

The Brock University Retirees Association was established in 1997. The Association seeks to provide retirees of the University with a forum that encourages them to maintain friendships; to keep its membership informed on matters concerning pension and benefits, and provide input as required; to assist the University with events in a mutually beneficial manner; and to provide retirees with other enrichment opportunities.

Retired Women Teachers of Ontario—St. Catharines branch fonds, 1970-2013.

The Retired Women Teachers of Ontario (RWTO) was founded in Toronto by a small group of retired women teachers known as the Rendezvous Club. This group brought together several branches in 1956 to form the Ontario Association of Superannuated Teachers (OAWST), which was changed to the RWTO in 1999. The group was initially formed in order to provide retired women teachers with the same pension that was received by their male colleagues. The group came to the realization that they would have a better chance of success if they had a larger group of supporters. As a result, new branches were formed throughout Ontario. In 1967, the government concurred that the pension should be raised, and the minimum pension level was increased to $1200 a year. The following year the Retired Teachers of Ontario was formed, representing both male and female retired teachers. This new group was now responsible for communicating and negotiating with the government concerning pension matters. However, the RWTO continued to exist with a focus on the special interests and well-being of retired women teachers. There are currently 53 branches throughout Ontario.

Barnes Wines, Limited By-Law No. 91

The summary states "a by-law respecting the borrowing of money, the issuing of securities and the securing of liabilities of Barnes Wines, Limited".

Bill no.71: An act respecting the Public Works of Ontario

Bill no.71: An act respecting the Public Works of Ontario (10 ½ pages, printed). S.D. Woodruff has signed this copy of the bill and has made a note in pension “regulations for management”, 1868.