Cloning, sequencing and characterization of a chitin synthase gene fragment from the fungus Phascolomyces articulosus /

Phascolomyces articulosus genomic DNA was isolated from 48 h old hyphae and was used for amplification of a chitin synthase fragment by the polymerase chain reaction method. The primers used in the amplification corresponded to two widely conserved amino acid regions found in chitin synthases of many fimgi. Amphfication resulted in four bands (820, 900, 1000 and 1500 bp, approximately) as visualized in a 1.2% agarose gel. The lowest band (820 bp) was selected as a candidate for chitin synthase because most amplified regions from other fimgi so far exhibited similar sizes (600-750 bp). The selected fragment was extracted from the gel and cloned in the Hinc n site of pUC19. The derived plasmid and insert were designated ^\5C\9'PaCHS and PaCHS respectively. The plasmid pUC19-PaC/fS was digested by several restriction enzymes and was found to contain BamHl and HincU sites. Sequencing of PaCHS revealed two intron sequences and a total open reading frame of 200 amino acids. The derived polypeptide was compared with other related sequences from the EMBL database (Heidelberg, Germany) and was matched to 36 other fiilly or partially sequenced fimgal chitin synthase genes. The closest resemblance was with two genes (74.5% and 73.1% identity) from Rhizopus oligosporus. Southern hybridization with the cloned fragment as a probe to the PCR reaction showed a strong signal at the fragment selected for cloning and weaker signals at the other two fragments. Southern hybridization with partially digested Phascolomyces articulosus genomic DNA showed a single band. The amino acid sequence was compared with sequences from other chitin synthase gene classes using the CLUSTALW program. The chitin synthase fragment from Phascolomyces articulosus was initially grouped in class n along with chitin synthase fragments from Rhizopus oligosporus and Phycomyces blakesleeanus which also belong to the same class, Zygomycetes. Bootstrap analysis using the neighbor-joining method available by CLUSTALW verified such classification. Comparison of PaCHS revealed conservation of intron positions that are characteristic of chitin synthase gene fragments of zygomycetous fungi.

Robert Campbell, Pelham Township Land Documents, 1879-1894 (not inclusive)

Robert Campbell was the son of Robert Campbell and Jannette Miller, born about 1836 in Pelham North Township. He married Elizabeth Ann Ingram on Saturday, September 7, 1878 in Pelham Township, and they had 2 sons: Robert Carson Campbell and George D. Campbell. He died on February 27, 1908 in Malahide Township, Norfolk County, Ontario.

Portrait of "Ruth", by George A. Hemstreet, Photographer, Milton [n.d.]

A woman identified only by the broach at her neck as "Ruth" is pictured here in a black and white cabinet card photograph by G. A. Hemstreet. The photo is undated, but believed to be from the late 1870s or early 1880s. The photographer's name is stamped in black ink along the bottom of the card: "G. A. Hemstreet, Photographer, Milton, Ont." This cabinet card was in the possession of Iris Sloman Bell, of St. Catharines, whose relatives include former Black slaves from the United States.G. A. Hemstreet, also known as George A. Hemstreet, was a photographer in Milton, Ontario from 1877 - 1887. Source: Phillips, Glen C. The Ontario photographers list (1851-1900). Sarnia: Iron Gate Publishing Co., 1990.

Letter from S.D. Woodruff to George Rykert

Letter from S.D. Woodruff which he writes from the office of the Port Dalhousie Thorold Railway Company to George Rykert. He submits an abstract of prices of people to undertake the construction of the railway from Port Dalhousie, Mar. 30, 1854.

Estimated cost of the Port Dalhousie and Thorold Railway sent to George Rykert by S.D. Woodruff

Estimated cost of the Port Dalhousie and Thorold Railway sent to George Rykert by S.D. Woodruff, Aug. 5, 1854.

Letter to S.D. Woodruff from George H. Gillespie regarding setting up a meeting of the members of the Long Point Company

Letter to S.D. Woodruff from George H. Gillespie regarding setting up a meeting of the members of the Long Point Company, Dec. 21, 1866.

Letter of notification of the annual meeting of the Long Point Company sent to S.D. Woodruff from George H. Gillespie

Letter of notification of the annual meeting of the Long Point Company sent to S.D. Woodruff from George H. Gillespie. Col. Clark is to present his proposal about timber on the point, June 24, 1868.

Letter to S.D. Woodruff from George H. Gillespie stating that Col. Clark has withdrawn his proposal about timber on the point

Letter to S.D. Woodruff from George H. Gillespie stating that Col. Clark has withdrawn his proposal about timber on the point. “Please send your views”, Aug. 10, 1868.

Letter of notification to S.D. Woodruff from George H. Gillespie that a meeting of the Board of Directors has been called

Letter of notification to S.D. Woodruff from George H. Gillespie that a meeting of the Board of Directors has been called, Oct. 20, 1869.

Letter to S.D. Woodruff from George H. Gillespie who says that Mackenzie has left many books and papers in the office

Letter to S.D. Woodruff from George H. Gillespie who says that Mackenzie has left many books and papers in the office. Mr. Gillespie makes inquiries about the purchase and license of Long Point. The letterhead on this document is “Gillespie and Powis, Commission Merchants, Brokers and Co., Hamilton, Ont.” (3 pages, handwritten), Apr. 30, 1878.

Letter to George H. Gillespie [from S.D. Woodruff] which is unsigned

Letter to George H. Gillespie [from S.D. Woodruff] which is unsigned (1 double-sided page, handwritten), in which he says that he cannot find the letter which was requested, Oct. 11, 1881.

Letter to S.D. Woodruff from A.C. Leslie and Co., of Montreal

Letter to S.D. Woodruff from A.C. Leslie and Co., of Montreal saying that the glass that he wants is not usually kept in stock, June 5, 1875.

Letter to S.D. Woodruff from George C. Flint and Co.

Letter to S.D. Woodruff from George C. Flint and Co., Upholsterers and Furniture Manufacturers of New York regarding items such as mantle glass, cornices and fabrics. They have enclosed a handwritten price list with this letter (4 pages, handwritten), Sept. 13, 1876.

Letter to S.D. Woodruff from George C. Flint and Co.

Letter to S.D. Woodruff from George C. Flint and Co. stating that they will deliver furniture to his house, Sept. 13, 1876.

Letter (copy) in three sections. The first part is addressed to Henry Nelles from M. Douglass. The second part is also addressed to Henry Nelles and is signed Mr. D. The third part is addressed to Mr. Griffen from George M. Richardson

Letter (copy) in three sections (1 ½ pages, handwritten). The first part is addressed to Henry Nelles from M. Douglass in which Mr. Douglass says he has enclosed $50. This section is dated Feb. 8, 1830. The second part is also addressed to Henry Nelles and is signed Mr. D. This is the section that says “your letter has just come stating that you had received a letter but the $50 was not in the letter. There was $50 in it when it left this post office. This is dated Feb. 14, 1830. The third part is addressed to Mr. Griffen from George M. Richardson. It says that this is to certify that Mr. Douglass did mail $50 on the 18th of this month. This letter is stained and slightly torn. Some of the text is affected. [This letter has to do with the case about the missing money which is included in this collection], Feb. 8, 1830.