Chart of station 2, crop sections of the old back ditch on the south side of the feeder, station 45, station 118 and the total length from the culvert to lot no. 5

Chart of station 2, crop sections of the old back ditch on the south side of the feeder, station 45, station 118 and the total length from the culvert to lot no. 5. This is signed by Fred Holmes, April 13, 1857.

Calculation of ditching in the south side of the feeder from Brown’s ditch culvert to lot no.5

Calculation of ditching in the south side of the feeder from Brown’s ditch culvert to lot no.5, n.d.

Molecular cloning restriction enzyme analysis, bovine adenovirus type 2 genome

Adenoviruses are non-enveloped icosahedral-shaped particles which possess a double-stranded DNA genome. Currently, nearly 100 serotypes of adenoviruses have been identified, 48 of which are of human origin. Bovine adenoviruses (BAVs), causing both mild respiratory and/or enteral diseases in cattle, have been reported in many countries all over the world. Currently, nine serotypes of SAVs have been isolated which have been placed into two subgroups based on a number of characteristics which include complement fixation tests as well as the ability to replicate in various cell lines. Bovine adenovirus type 2 (BAV2), belonging to subgroup I, is able to cause pneumonia as well as pneumonic-like symptoms in calves. In this study, the genome of BAV2 (strain No. 19) was subcloned into the plasmid vector pUC19. In total, 16 plasmids were constructed; three carry internal San fragments (spanning 3.1 to 65.2% ), and 10 carry internal Pstl fragments (spanning 4.9 to 97.4%), of the viral genome. Each of these plasmids was analyzed using twelve restriction endonucleases; BamHI, CiaI, EcoRl, HiOOlll, Kpnl, Noll, NS(N, Ps~, Pvul, Saj, Xbal, and Xhol. Terminal end fragments were also cloned and analyzed, sUbsequent to the removal of the 5' terminal protein, in the form of 2 BamHI B fragments, cloned in opposite orientations (spanning 0 to 18.1°k), and one Pstll fragment (spanning 97.4 to 1000/0). These cloned fragments, along with two other plasmids previously constructed carrying internal EcoRI fragments (spanning 20.6 to 90.5%), were then used to construct a detailed physical restriction map using the twelve restriction endonucleases, as well as to estimate the size of the genome for BAV2(32.5 Kbp). The DNA sequences of the early region 1 (E1) and hexon-associated gene (protein IX) have also been determined. The amino acid sequences of four open reading frames (ORFs) have been compared to those of the E1 proteins and protein IX from other Ads.

Second Welland Canal - Book 3, Survey Map 14 - Village of Junction and Crowland

Survey map of the Second Welland Canal created by the Welland Canal Company showing the border area of the townships of Crowland and Humberstone, as well as the Village of Junction. Identified structures associated with the Canal include ditches, guard lock, old canal, new towing path, bridge, feeder to Dunnville, covered drain. Surveyor measurements and notes can be seen in red and black ink and pencil. Local area landmarks include James Turf Tavern and possible marshland. Roads parallel to Canal include western Road Allowance, the new towing road, road to Welland and road to Junction. Roads perpendicular to Canal include Road Allowance between the 5th and 6th Concession. Properties and property owners are noted as Thomas. C. Street, James Tuft, and John Hellems. Lots noted are: Lots Number 26, 27, 6th Concession.

Second Welland Canal - Book 3, Survey Map 15 - Village of Junction and Crowland

Survey map of the Second Welland Canal created by the Welland Canal Company showing the border area of the townships of Crowland and Humberstone, as well as the Village of Junction. Identified structures associated with the Canal include ditches, Junction Lock, bridge, feeder to Marshville, and spoil banks. Surveyor measurements and notes can be seen in red and black ink and pencil. Local area landmarks include the Gore between Crowland and Humberstone, pond, creek, H. Hellems Wharf Lot, John Toyne property, School House, Tavern, Barn, and House. Roads parallel to Canal include southern Road Allowance and the Road to Port Colborne. Roads perpendicular to Canal include Road Allowance between the 6th and 7th Concession. Properties and property owners are noted as Thomas Street, John Hellems, James Boyd, John Toyne, and F. Holmes. Lots noted are: Lots Number 25, 26, 27, 7th Concession.

Welland Canal Survey of Lands Jonathan Silverthorn, 1834

Survey map and description of Jonathan Silverthorn's land created by The Welland Canal Company. Included is a written description of the land along with a drawing of the land. The lands surveyed include lots 229 and 230. The land was first surveyed in 1830, then again in 1834, by George Keefer. The original survey only included the feeder and resevoir and wood land, whereas the second survey shows all lands owned by Silverthorn. The land totals 19.2 acres, 2 roads and 32 perches. The land is broken down as follows; 7.6 acres cleared land, canal and towpath, 6.6 acres reservoir - Michael Silverthorn, 5 acres woodland. Surveyor notes are seen in pencil and red pen on the map.See also page 138.

Welland Canal Survey of Lands Company Land in Dunnville, 1834

Survey map and description of the land belonging to the Welland Canal Company at Dunnville. Created by The Welland Canal Company. Included is a written description of the land along with a drawing of the land. There are two seperate surveys done for the lands: Survey #1 (Pp. 148-149) noteable features include; the Grand River, the original boundry of the Grand River, marsh overflow, marsh, feeder river, bridge, Broad street, Lock street, Main street, embankment, dam (600 ft.), lines between lots, reserve for the ships yard, reserve for lock and dry dock, lands occupied by the canal and towpath to guard gate. The land totals 9 acres, and 3 roads, including the street. Survey #2 (Pp. 150-151) completed by George Keefer noteable features include; embankment, marsh overflow, original channel of the Grand River, salt spring, bridges, wier, proposed waste wier, Van Riper's home, proposed bridge, sulphur spring, road, Sulphur Creek, division between lots 12-17. The land totals 27 acres, and 2 perches. Surveyors notes can be seen in pencil and red ink on the survey.See also Pp. 148-151

Welland Canal Survey of Lands Village of Marshville - Milton, 1835

Survey map of the plan of the Village of Marshville, created by The Welland Canal ompany. Included is a two page drawing of the land. Noteable features include; line between 5th and 4th concessions, William Simpson's land, line between 3rd and 4th concessions, feeder, bridges, mill lots, road to Sugarloaf, old stakes, Canby lot 17, lot no.19, lot divisions. The drawing is titled "Plan of the Village of Marshville now Milton, reserveyed September 18th, 1835". Surveyor notes are seen in pencil on the map.See Pages 164-165

Welland Canal/St. Lawrence Seaway Authority fonds, 1826-1880, 1905-1951

The Welland Canal Company was formed in 1824 by William Hamilton Merritt. Construction of the first Welland Canal began in 1829 and was completed in 1834. The canal ran south from Port Dalhousie along Twelve Mile Creek to St. Catharines. An extension was built in 1833 to Gravelly Bay, now Port Colborne. As ships became larger and the wooden locks deteriorated, the need for a new canal became apparent. In 1839, the government purchased the Welland Canal Company’s assets and began making plans for the construction of a second canal. Construction began in 1841 and was completed by 1845. In 1887, a third Welland Canal was completed, which operated until 1932, when a fourth canal was completed. This canal remains in operation today.

Habituating Antelope and Bison to Cooperate with Veterinary Procedures

A study that involved conditioning antelope and bison calves over a a number of days so that the animals would not panic so that veterinarian procedures like blood sampling would be performed on a calm animal.

Letter to S.D. Woodruff from Fred Holmes

Letter to S.D. Woodruff from Fred Holmes in which he tells Mr. Woodruff that the back ditch on the south side of the feeder is full of water, April 13, 1857.

Diagrams (charts and graphs) made into a booklet with a newspaper cover

Diagrams (charts and graphs) made into a booklet with a newspaper cover. This booklet contains cross sections of the back ditch on the south side of the Welland Canal feeder, west of the Marshville culverts (45 pages, hand drawn). This was created by Fred Holmes, Oct. 3, 1857.

Charts and graphs of cross sections from Brown’s ditch culvert to the main drain

Charts and graphs of cross sections from Brown’s ditch culvert to the main drain, cross sections from the feeder on the road allowance between lots 26 and 27 in the 5th concession of Humberstone, Cross sections of the main drain from Lyons Creek culvert to the road allowance between lots 7 and 8 in Wainfleet and cross selections of the old ditch on the west side of the road allowance between lots 17 and 18 in the 3rd concession in Wainfleet (8 pages, hand drawn), n.d.

Letter (unsigned) from the Welland Canal Office

Letter (unsigned) from the Welland Canal Office regarding a quantity of wood felled upon the bank of the feeder. It is laid too near the back ditch. The recipient of the letter is instructed to proceed with the removal of the wood so that the excavation of the ditch might proceed, Aug. 7, 1858.