Biotransformations of water insoluble substrates in aqueous, two-phase and encapsulated systems /

The biotransformation of water insoluble substrates by mammalian and bacterial cells has been problematic, since these whole cell reactions are primarily performed in an aqueous environment The implementation of a twophase or encapsulated system has the advantages of providing a low water system along with the physiological environment the cells require to sustain themselves. Encapsulation of mammalian cells by formation of polyamide capsules via interfacial polymerization illustrated that the cells could not survive this type of encapsulation process. Biotransformation of the steroid spironolactone [3] by human kidney carcinoma cells was performed in a substrate-encapsulated system, yielding canrenone [4] in 70% yield. Encapsulation of nitrile-metabolizing Rhodococcus rhodochrous cells using a polyamide membrane yielded leaky capsules, but biotransformation of 2-(4- chlorophenyl)-3-methylbutyronitrile (CPIN) [6] in a free cell system yielded CPIN amide [7] in 40% yield and 94% ee. A two-phase biotransformation of CPIN consisting of a 5:1 ratio of tris buffer, pH 7.2 to octane respectively, gave CPIN acid [8] in 30% yield and 97% ee. It was concluded that Rhodococcus rhodochrous ATCC 17895 contained a nonselective nitrile hydratase and a highly selective amidase enzyme.

The impact of hospital downsizing on registered nurses displaced from full-time employment

This research identified and explored the various responses often women Registered Nurses displaced from full-time elnployment as staff nurses in general hospitals in southern Ontario. These nurses were among the hundreds in Ontario who were displaced between October 1991 and October 1995 as a result of organizational downsizing and other health care reform initiatives. The purpose ofthis research was to document tIle responses of nurses to job displacement, and how that experience impacted on a nurse's professional identity and her understanding of the nature and utilization of nursing labour. This study incorporated techniques consistent with the principles of naturalistic inquiry and the narrative tradition. A purposive sample was drawn from the Health Sector Training and Adjustment Program database. Data collection and analysis was a three-step process wherein the data collection in each step was informed by the data analysis in the preceding step. The main technique used for qualitative data collection was semistructured, individual and group interviews. Emerging from the data was a rich and textured story ofhow job displacement disrupted the meaningful connections nurses had with their work. In making meaning of this change, displaced nurses journeyed along a three-step path toward labour adjustment. Structural analysis was the interpretive lens used to view the historical, sociopolitical and ideological forces which constrained the choices reasonably available to displaced nurses while Kelly's personal construct theory was the lens used to view the process of making choices and reconstruing their professional identity.

Dianna Hostetter funeral invitation, 1861

The invitation is a request of Jacob Hostetter to his friends and acquaintances to attend the funeral of his wife, Dianna on Tuesday, October 29, 1861. Dianna, also known as Dinah, was the second daughter of Joseph and Mary Heslop Van Every. She was born in 1831 and married Jacob Hostetter of Grantham Township. Jacob died a year later, leaving two children, Joseph Blain Hostetter (1860-1896) and Laura Diana Hostetter (1861-1933). Jacob was the son of Capt. Herman Hostetter of Ten Mile Creek who had died from wounds received at the Battle of Queenston Heights. Jacob and Dianna Hostetter are buried in the Warner Cemetery, Niagara Falls, Ont.

The Impact of Hospital Downsizing on Registered Nurses Displaced from Full -Time Employment

This research identified and explored the various responses of ten women Registered Nurses displaced from full-time employment as staff nurses in general hospitals in southern Ontario. These nurses were among the hundreds in Ontario who were displaced between October 1991 and October 1995 as a result of organizational downsizing and other health care reform initiatives. The purpose of this research was to document the responses of nurses to job displacement, and how that experience impacted on a nurse's professional identity and her understanding of the nature and utilization of nursing labour. This study incorporated techniques consistent with the principles of naturalistic inquiry and the narrative tradition. A purposive sample was drawn from the Health Sector Training and Adjustment Program database. Data collection and analysis was a three-step process wherein the data collection in each step was informed by the data analysis in the preceding step. The main technique used for qualitative data collection was semistructured, individual and group interviews. Emerging from the data was a rich and textured story of how job displacement disrupted the meaningful connections nurses had with their work. In making meaning of this change, displaced nurses journeyed along a three-step path toward labour adjustment. Structural analysis was the interpretive lens used to view the historical, sociopolitical and ideological forces which constrained the choices reasonably available to displaced nurses while Kelly's personal construct theory was the lens used to view the process of making choices and reconstruing their professional identity.

Wakeman Burritt fonds, 1807-1814

Wakeman Burritt (1785-1847) was a merchant and ship owner who conducted his business in New York, New Orleans, LA, Charleston, SC, and the West Indies. Despite the difficulties that the Embargo Act of 1807 and the War of 1812 posed to trade and commerce, Burritt managed to run a successful business, dealing in commodities such as foodstuffs, cloth, cotton, and soap. He owned three ships, the Brig Cannon, Brig Eunice and Brig Sampson. In 1809, he married Grace Burr (1787-1874). Together they had at least one child, Francis Burritt (1811-1861).

Wakeman Burritt Fonds

Fonds consists of correspondence between Wakeman Burritt and his business associates, as well as receipts and documents concerning the shipping vessels Brig Cannon, Brig Eunice, Brig Sampson, and several other vessels. Reference is made in some documents to the effects of politics on commerce, most notably the Embargo Act of 1807 and the War of 1812. The correspondence consists of 53 letters, 40 of the letters being addressed to Wakeman Burritt, with the remaining 13 letters being retained copies of letters written by Burritt to Samuel Cannon. There are also 17 letters written to Burritt by LeGrand Cannon of Norwalk, CT. The remaining letters to Burritt consist of two letters from his wife Grace Burritt, and other business associates, including Thomas Andrews, Stephen Arnold, Ezra Burr, Talcott Burr, Talmon Burr, Wm. M. Betty & Co., Samuel Cannon, Edward Jessup, Hugh Knox, Jonathan Meigs, Samuel Morehouse, Stephen Morehouse, W & B St. John, Oliver Sherman, Anthony Stow, and Ogden Richards Weldon. The shipping vessel material includes 52 documents concerning Brig Eunice, 27 documents for Brig Sampson, 14 documents for Brig Cannon, and 18 miscellaneous documents for vessels such as the Schooners Maria, Hope, Nassau, and Henry, Sloops Fox and Sally, and the Brig Diana and Emeline. The documents include receipts for piloting into port, for wharfage, and for supplies and repairs, as well as lists of disbursements, freight lists, and payments made to men working on the ships.