8 resultados para Blunt, Wilfrid

em Brock University, Canada


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A letter from Prime Minister Wilfrid Laurier to A. Munro Grier regarding an invitation to His Royal Highness. This is possibly referencing the Royal visit of the Duke of Cornwall in the year 1901. The Royals were due to tour Canada September and October of that year, with a stop in the Niagara area October 13th.

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March 3, 1868. -- Referred to the Select Committee on the Niagara Ship Canal.

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The manipulation of large (>10 kb) plasmid systems amplifies problems common to traditional cloning strategies. Unique or rare restriction enzyme recognition sequences are uncommon and very rarely located in opportunistic locations. Making site-specific deletions and insertions in larger plasmids consequently leads to multiple step cloning strategies that are often limited by time-consuming, low efficiency linker insertions or blunt-end cloning strategies. Manipulation ofthe adenovirus genome and the genomes ofother viruses as bacterial plasmids are systems that typify such situations. Recombinational cloning techniques based on homologous recombination in Saccharomyces cerevisiae that circumvent many ofthese common problems have been developed. However, these techniques are rarely realistic options for such large plasmid systems due to the above mentioned difficulties associated with the addition ofrequired yeast DNA replication, partitioning and selectable marker sequences. To determine ifrecombinational cloning techniques could be modified to simplify the manipulation of such a large plasmid system, a recombinational cloning system for the creation of human adenovirus EI-deletion rescue plasmids was developed. Here we report for the first time that the 1,456 bp TRP1/ARS fragment ofYRp7 is alone sufficient to foster successful recombinational cloning without additional partitioning sequences, using only slight modifications of existing protocols. In addition, we describe conditions for efficient recombinational cloning involving simultaneous deletion of large segments ofDNA (>4.2 kb) and insertion of donor fragment DNA using only a single non-unique restriction site. The discovery that recombinational cloning can foster large deletions has been used to develop a novel recombiliational cloillng technique, selectable inarker 'kilockouf" recombinational cloning, that uses deletion of a yeast selectable marker coupled with simultaneous negative and positive selection to reduce background transformants to undetectable levels. The modification of existing protocols as described in this report facilitates the use of recombinational cloning strategies that are otherwise difficult or impractical for use with large plasmid systems. Improvement of general recombinational cloning strategies and strategies specific to the manipulation ofthe adenovirus genome are considered in light of data presented herein.

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This research paper examines themes of power and privilege that occur within service-learning as described by 3 Ontario universities on their service-learning websites. Due to size and time restrictions, this paper was able to examine only 3 Ontario universities: Brock, Wilfrid Laurier, and Lakehead. The purpose of this study is geared towards service-learning practitioners in order for the universities and students to become more self-aware of their immense place of privilege within the service-learning context. Qualitative narrative analysis research methods were employed in this purposeful sample to examine how each university’s story of service-learning reflected themes of power and privilege. The research found that each university posed a unique narrative of service-learning representing various stakeholders’ voices and presence in different ways on their website. Brock largely focuses on faculty and student voices. Laurier intentionally attempts to include all three stakeholder voices, although still favours students and the university as an audience over the community. Lakehead’s unique program includes a plethora of voices and intends much of their information for the community members, students, and the university. The implications of this research demonstrate that universities have a large amount of power and privilege, which is carried through to the students within the service-learning partnership.

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Christopher Newton was born in England in June of 1936. He received his education at Sir Roger Manwood’s School in Kent, the University of Leeds, Purdue University in Indiana and the University of Illinois where he received his M.A. He founded Theatre Calgary in 1968 and was the artistic director there until 1971. He was appointed as the artistic director of the Vancouver Playhouse where he established the Playhouse Acting School with Powys Thomas. Mr. Newton has also performed and directed at Stratford Festivals and on Broadway. He became the artistic director at Shaw Festival in Niagara-on-the-Lake in 1979 and remained there for 23 seasons until 2002. Mr. Newton has many television, radio and film credits to his name as well as having written many stage plays. Mr. Newton has received honorary degrees from Brock University (1986), the University of Guelph (1989), Wilfrid Laurier University (1997) and Buffalo State University. He was made an Honorary Fellow at the Royal Conservatory of Music of Toronto (1993) and of Ryerson Polytechnic University (1995). He has won the Governor General’s performing arts award (2000), the Molson Prize and the Thomas DeGaetani Award from the United States Institute for Theatre Technology. In 1996 he was made an Honorary Life Member of the Association for Canadian Theatre Research and in the same year he received the M. Joan Chalmers Award for Artistic Direction. In 1995, he was made a Member of the Order of Canada and he won a Governor General's Performing Arts Award in 2000. Christopher Newton is currently the Artistic Director Emeritus at the Shaw Festival. Sources: http://www.shawfest.com/the-ensemble/christopher-newton/ http://www.thecanadianencyclopedia.com/articles/christopher-newton

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The Second U.S. Regiment of Light Dragoons was formed in January 1812. In March of 1814, it merged with the First U.S. Regiment of Light Dragoons to form the Regiment of Light Dragoons.

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An orderly book of the Second Regiment of U.S. Dragoons, New York, dated August 14, 1812-July 29, 1813. The book contains orders pertaining to day-to-day military matters, such as punishments for disobedience, court-martial proceedings, camp rules and regulations, and guidelines for interacting with civilians in the vicinity of the camp. The Regiment was stationed at various locations in upstate New York and Canada, including Greenbush, Albany, Sackets Harbor, Utica, Geneva, Fort Niagara and Fort George. General Henry Dearborn originally commanded the Regiment at Greenbush. Names noted in this book include:E. Beebe, Deputy Adjt. General; William King, Capt. 15th; John Chandler, General ;W. Gamewood, Major ;James Burns, Colonel;John Woodford, Major; Andrew McDowell, Capt.; Abm. Gustis, Major; C.W. Hunter, Brigade Major; Selden, Captain; Holland, Captain; Harris, Captain; Clarkson, Lieutenant; Johnson, Lieutenant; Robert Craig, Adjt.; R.G. Hith, A.A. General. Also included with the orderly book are a monthly return form, a contract for medical services, and a bonus pay voucher for Thomas Blunt. The monthly return form is partially completed and dated January 1813 at Greenbush, New York. It is signed by Captain Jonas Holland. The contract is dated May 20, 1812, between John Dodge, physician and surgeon, and Jonas Holland. The contract describes the services required of the physician and the salary to be paid. The bonus pay voucher is dated April 25, 1813, for $8.00 paid to Thomas Blunt by Captain Jonas Holland for 'enlisting into the army of the United States for five years'.

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Brass Surveying Compass with a 6 1/2 inch diameter silvered brass dial signed E. and G.W. Blunt of New York. There is a fleur-de-lis at the north point and the outer needle ring is engraved 0-90 in four quadrants. There is an engraved central pattern, blue steel hand and brass lifter, shaped limbs and a brass dial cover. This belonged to Samuel DeVeaux Woodruff who was a Civil Engineer. It also belonged to R.D.W. Band who was also a Civil Engineer. Also, One 13 cm. brass spindle taper for the surveying compass, n.d