Two leaves from a Medieval breviary (prayer-book), c.1350-1400

The actual scripture quotations begin with blue and gold illuminated letters. Towards the end of each passage, there is a small red “R” that marks the “Response”. At the conclusion of the Response, the next lesson is announced. There are three lessons on these leaves. The first leaf begins with the word “Requiem” with the initial letter illuminated. The third lesson begins with the illuminated letter “M” on the word “Manus”. This passage is from the Book of Job, Chapter 10, verses 8-11 which reads: Your hands have formed me and fashioned me; will you then turn and destroy me? Oh, remember that you fashioned me from clay. Will you then bring me down to dust again? Did you not pour me out as milk, and thicken me like cheese? With skin and flesh you clothed me, with bones and sinews knit me together.

STRATEGIES FOR PREVENTION AND TREATMENT OF HEPATITIS C VIRUS INFECTION

Hepatitis C virus (HCV) is the causative agent of Hepatitis C, a serious global health problem which results in liver cirrhosis and hepatocellular carcinoma. Currently there is no effective treatment or vaccine against the virus. Therefore, development of a therapeutic vaccine is of paramount importance. In this project, three alternative approaches were used to control HCV including a DNA vaccine, a recombinant viral vaccine and RNA interference. The first approach was to test the effect of different promoters on the efficacy of a DNA vaccine against HCV. Plasmids encoding HCV-NS3 and E1 antigens were designed under three different promoters, adenoviral E1A, MLP, and CMV ie. The promoter effect on the antigen expression in 293 cells, as well as on the antibody level in immunized BALB/c mice, was evaluated. The results showed that the antigens were successfully expressed from all vectors. The CMV ie promoter induced the highest antigen expression and the highest antibody level. Second, the efficiency of a recombinant adenovirus vaccine encoding HCV-NS3 was compared to that of a HCV-NS3 plasmid vaccine. The results showed that the recombinant adenovirus vaccine induced higher antibody levels as compared to the plasmid vaccine. The relationship between the immune response and miRNA was also evaluated. The levels of mir-181, mir-155, mir-21 and mir-296 were quantified in the sera of immunized animals. mir-181 and mir-21 were found to be upregulated in animals injected with adenoviral vectors. Third, two recombinant adenoviruses encoding siRNAs targeting both the helicase and protease parts of the NS3 region were tested for their ability to inhibit NS3 expression. The results showed that the siRNA against protease was more effective in silencing the HCV-NS3 gene in a HCV replicon cell line. This result confirmed the efficiency of adenovirus for siRNA delivery. These results confirmed that CMV ie is optimum promoter for immune response induction. Adenovirus was shown to be an effective delivery vector for antigens or siRNAs. In addition, miRNAs were proved to be involved in the regulation of immune response.

HOEK UNIT C (Y)

Light brown sediment with mainly small clasts. The clast shape ranges from sub-angular to sub-rounded. Lineations are abundant throughout the sample. Edge-to-edge grain crushing, as well as crushed grains, can also be seen.

HOEK UNIT C (Z) B3

Light brown sediment with clasts ranging from small to medium in size. The clast shape ranges from sub-angular to rounded. Lineations and edge-to-edge grain crushing can mainly be seen in this sample. There are also a few examples of grain stacking and crushed grains.

HOEK UNIT C 81

Light brown sediment with clast sizes ranging from small to medium. Clast shape ranges from sub-angular to sub-rounded. Lineations are common throughout the sample. Minor amounts of edge-to-edge grain crushing can also be seen.

Letter to Mr. C. Howard from Mrs. Tolan

Letter (3 typed pages) to Mr. C. Howard of the Dominion Bank of Toronto from Mrs. Tolan of the Lundy’s Lane Historical Society. She encloses 2 articles entitled “Origins of Niagara Falls, Ontario” and “Captain Creighton and Clifton” Samuel Zimmerman is named in the first article. Mrs. Tolan requests that Mr. Band is also allowed to read the articles, April 25, 1948.

Letter to S.D. Woodruff from George C. Flint and Co.

Letter to S.D. Woodruff from George C. Flint and Co. stating that they will deliver furniture to his house, Sept. 13, 1876.

Letter to Joseph A. Woodruff from E.C. Thomas

Letter to Joseph A. Woodruff from E.C. Thomas saying that he intends to send the money which is owed to Mr. Woodruff, Dec. 4, 1865.

Letter to Robert Nelles from James C. Henderson and Co.

Letter to Robert Nelles from James C. Henderson and Co. requesting money, Nov. 25, 1850.

Letter to Robert F. Nelles from James C. Henderson and Co

Letter to Robert F. Nelles from James C. Henderson and Co. acknowledging payment, Jan. 25, 1851.

Promissory note to James C. Henderson and Co. signed by Robert F. Nelles

Promissory note to James C. Henderson and Co. signed by Robert F. Nelles, May 8, 1850.

Letter to J. Bradley from B.C.A. Gogy

Letter to J. Bradley from B.C.A. Gogy in the Office of the Adjutant General of Militia, Montreal (1 page, printed) regarding Bradley’s desire to obtain commission as a captain in the Quebec Artillery, Aug. 25, 1845

Letter to J.P. Bradley from B.C.A. Gogy

Letter to J.P. Bradley from B.C.A. Gogy in the Office of the Adjutant General of Militia, Montreal regarding procedures for applying for a captain’s commission in the militia (2 ½ pages, handwritten), Dec. 23, 1845.