4 resultados para 235

em Brock University, Canada


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Taken for Dialogue in 1984.

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The Fonthill Grammar School was established in 1856. The school closed in 1876 when it failed to receive community support for a new building. The editors of the journal were two individuals with the last names Ray, and Wiggins, unfortunately their first names are not included in any of the submissions to the journal.

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This letter is very faded and difficult to read. It mentions that she leaves one letter on a counter while picking up a parcel and the there is a written note included: "Miss Reynolds - Am holding a letter you left on my counter when you bought these towels - will hold it until you call -" This letter is labelled number 235.

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Acute alterations in cell volume can substantively modulate subsequent metabolism of substrates. However, how such alterations in skeletal muscle modulate protein metabolism is limited. The purpose of this study was to determine the time dependent influence of extracellular osmotic stress on protein turnover in skeletal muscle cells. L6 cells were incubated in hyperosmotic (HYPER; 425.3 ± 1.8mmol/kg), hypo-osmotic (HYPO; 235.4 ± 1.0mmol/kg) or control (CON; 333.5 ± 1.4mmol/kg) media for 4, 8, 12, or 24hrs. During the final 4hrs, incorporation of L-[ring-3,5-3H]-tyrosine was measured to estimate protein synthesis. Western blotting measured markers of protein synthesis and degradation. No differences were observed in any outcomes except p70S6K phosphorylation whereby HYPO was lower (p<0.05) than CON and HYPER; which remained similar except for a large increase at 8hrs for HYPER. These findings suggest that regardless of duration, extracellular osmotic stress does not significantly affect protein metabolism in L6 cells.