Analysis of Alpha CA Genes in Early Vertebrates and ; High-Invertebrates

Carbonic anhydrases are enzymes that are ubiquitously found in all organisms that are engaged in catalyzing the hydration of carbon dioxide to form bicarbonate and proton and vice versa. They are crucial in the process of respiration, bone resorption, pH regulation, ion transport, and photosynthesis in plants. Out of the five classes of carbonic anhydrase α, β, γ, δ, ζ this study focused in the α carbonic anhydrases. This class of CAs constitute of 16 subfamilies in mammals that include 3 non-active enzymes known as Carbonic Anhydrase Related Proteins. The inactiveness of these enzymes is due to the loss of one or more Histidine residues in the active site. This thesis was conducted based on the aim of studying evolutionary analysis of carbonic anhydrase sequences from organisms spanning from the Cambrian age. It was carried out in two phases. The first phase was the sequence collection, which involved many biological sequence databases as a source. The scope of this segment included sequence alignments and analysis of the sequence manually and in an automated form incorporating few analysis tools. The second Phase was phylogenetic analysis and exploring the subcellular location of the proteins, which was key for the evolutionary analysis. Through the medium of the methods conducted with respect to the phases mentioned above, it was possible to accomplish the desired result. Certain thought-provoking sequences were come across and analyzed thoroughly. Whereas, Phylogenetics showed interesting results to bolster previous findings and new findings as well which lay bedrock for future intensified studies.

A longitudinal study on the lexical sophistication and sentence length in Finnish upper secondary school students’ English compositions.

The aim of the present study was to find out the level of lexical sophistication and the mean length of sentences used in compositions written by Finnish upper secondary school students of English. In addition, the present study investigated the possible relationship between the two abovementioned variables. The study at hand was longitudinal: as data, a set of 50 compositions were collected in 2014 from the same writers both in the first and the final year of upper secondary school, 25 in the first year and 25 in the final year. In the analysis, an internet-based program called VocabProfile was utilized in order to find out the lexical sophistication of the investigated students. To find out the mean length of sentence and the relationship between these two, I used Microsoft Excel. Findings of the present study include a minor decrease in the use of less frequent vocabulary and a slight increase in the use of the two most frequently appearing thousand words of English: both of these changes were 1.99 percentage points. As for the mean length of sentence, it grew by 1.28 words during upper secondary school. As for the relationship between the two variables, no clear correlations could be found. It became, however, relatively clear that the topic of the composition might have an effect on the results. Thus more research is needed to fully see the effect of lexical sophistication and mean length of sentence on one another. In addition, future research would benefit greatly if all investigated students wrote on the same topic.