Incest aversion : the evolutionary roots of individual regulation

Den evolutionära förklaringen till den allmänt utbredda incestaversionen, dvs. motviljan för sex med nära släktingar, försöker besvara såväl frågan om varför incestaversionen gynnats i det naturliga urvalet som frågan om hur denna aversion regleras på individuell nivå. Eftersom inavlade barn har en försämrad biologisk duglighet än andra barn, medför kostnaderna av denna icke-optimala reproduktion ett selektionstryck mot inavel. I en serie studier visade vi att eftersom kvinnor i allmänhet satsar mer biologiska resurser på sina barn än vad män gör, känner kvinnor starkare incestaversion än män och eftersom det endast är fertila kvinnor som riskerar satsa resurser i en inavlad avkomma har även fertila kvinnor högre incestaversion än icke-fertila kvinnor. Vi visade också att de biologiska kostnaderna av inavel inte begränsas till enbart de individer som har incest. Eftersom alla våra biologiska släktingar sannolikt delar våra alleler speglar incestaversionen även de biologiska kostnader som incest mellan våra släktingar medför åt oss. Den psykologiska mekanism med vilken incestsituationer bedöms har hittills varit okänd. I våra studier testades teorin om självreflekterande empati. Enligt den teorin bedöms sådana situationer emotionellt genom att man själv föreställer sig ha sex med motsvarande släkting och känslan som väcks i denna process ger därefter emotionell information till bedömningen av andras incest. I tre delstudier fann vi att självreflektion var positivt associerat med styrkan av aversion gentemot andras incest, vilket stöder teorin om självreflekterande empati.

Alpha- and gamma-melanocyte stimulating hormones in obesity. A study of the key central areas of metabolic regulation

The melanocortin system is an important regulator of feeding, energy metabolism,and cardiovascular function and it consists of the pro-opiomelanocortin (POMC) derived melanocyte stimulating hormones (α-, β- and γ-MSH) and their endogenous melanocortin receptors, MC1R to MC5R. In the hypothalamus, α-MSH reduces food intake, and increases energy expenditure and sympathetic tone by binding to MC4R. Mutations affecting the MC4R gene lead to obesity in mammals. On the other hand, the metabolic effects of MC3R stimulation using agonists such as the endogenously expressed γ-MSH have been less extensively explored. The main objective of this study was to investigate the long-term effects of increased melanocortin tone in key areas of metabolic regulation in the central nervous system (CNS) in order to investigate the sitespecific roles of both α-MSH and γ-MSH. The aim was to stereotaxically induce local overexpression of single melanocortin peptides using lentiviral vectors expressing α-MSH (LVi-α-MSH-EGFP) and γ-MSH (LVi-γ-MSH-EGFP). The lentiviral vectors were shown to produce a long-term overexpression and biologically active peptides in cell-based assays. The LVi-α-MSHEGFP was targeted to the arcuate nucleus in the hypothalamus of diet induced obese mice where it reduced weight gain and adiposity independently of food intake. When the nucleus tractus solitarus in the brainstem was targeted, the LVi-α-MSH-EGFP treatment was shown to cause a small decrease in adiposity, which did not impact weight development. However, the α-MSH treatment increased heart rate, which was attenuated by adrenergic receptor blockade indicative of increased sympathetic activity. The LVi-γ-MSH-EGFP was targeted to the hypothalamus where it decreased fat mass in mice eating the standard diet, but the effect was abated if animals consumed a high-fat Western type diet. When the diet induced obese mice were subjected again to the standard diet, the LVi-γ-MSH-EGFP treated animals displayed increased weight loss and reduced adiposity. These results indicate that the long-term central anti-obesity effects of α-MSH are independent of food intake. In addition, overexpression of α-MSH in the brain stem efficiently blocked the development of adiposity, but increased sympathetic tone. The evidence presented in this thesis also indicates that selective MC3R agonists such as γ-MSH could be potential therapeutics in combination with low fat diets.

Value Creation of Professional Services

The aim of this qualitative thesis is to research, how professional services create value, and what are the key factors affecting on this process. A model describing value creation process in professional services will be explained, based on the existing literature and three in depth discussions with professional service representatives. Professional services are such services that are tend to base on problem solving and require high skills and knowledge of a professional. These services often have a consultative or an advisory nature, and they often involve both client and a company in service process. As the service is often delivered in somewhat collaboration so is the value created. The results of this thesis revealed that value is created in collaboration or co-operation in professional services. Therefore, value co-creation, as a concept, is exact to describe the value creation process in professional services. This thesis also revealed that value is co- created in continuous communication between a client and a company and no value or only a little value can be created if there is no effective communication during the professional service process.