Mode of forcing a passage through the ice

Englantilaisen tutkimusmatkaajan E. D. Clarken Ahvenanmeren ylitys joulukuussa 1799. - Digitoitu valokuvasta, joka julkaistu kirjassa: R. Knapas & P. Koistinen, Historiallisia kuvia, 1993.

Perilous passage on the ice

London : Joseph Mawman 1802

Gut Microbiota and Metabolic Disorders

Obesity and its co-morbidities, such as metabolic syndrome (MetS), non-alcoholic fatty liver disease (NAFLD) and type 2 diabetes, have increased over the last few decades like an epidemic. So far the mechanisms of many metabolic diseases are not known in detail and currently there are not enough effective means to prevent and treat them. Several recent studies have shown that the unbalanced gut microbiota composition (GMC) and activity have an influence on the fat accumulation in the body. Further, it seems that the GMC of obese individuals differs from the lean. The aim of this study was to investigate whether there are differences between the GMC of metabolically impaired overweight/obese (MetS group), metabolically healthy overweight/obese and normal-weight individuals. In addition, the mechanisms by which the gut bacteria as well as their specific structures, such as flagellin (FLG) that stimulates the Toll-like receptor 5 (TLR5) affect metabolism, were investigated both in vivo and in vitro in human adipocytes and hepatocytes. The results of this study show that the abundance of certain gram-positive bacteria belonging to the Clostridial cluster XIV was higher in the MetS group subjects compared to their metabolically healthy overweight/obese and lean counterparts. Metabolically impaired subjects tended to also have a greater abundance of potentionally inflammatory Enterobacteria in their gut and thus seemed to have aberrant GMC. In addition, it was found that subjects with a high hepatic fat content (HHFC group) had less Faecalibacterium prausnitzii in their gut than individuals with low hepatic fat content. Further gene expression analysis revealed that the HHFC group also had increased inflammation cascades in their adipose tissue. Additionally, metabolically impaired individuals displayed an increased expression of FLG-recognizing TLR5 in adipose tissue, and the TLR5 expression levels associated positively both with liver fat content and insulin resistance in humans. These changes in the adipose tissue may further contribute to the impaired metabolism observed, such as insulin resistance and dyslipidemia. In vitro -studies showed that the FLG-induced TLR5 activation in adipocytes enhanced the hepatic fat accumulation by decreasing insulin signaling and mitochondrial functions and increasing triglyceride synthesis due to increased glycerol secretion from adipocytes. In conclusion, the findings of this study suggest that it may be possible that the novel prevention and personalized treatment strategies based on GM modulation will succesfully be developed for obesity and metabolic disorders in the future.

Ich hoffe dass alles da gut lauft. Eine Analyse von Aufsätzen finnischer Gymnasiasten

Diese Pro-Gradu-Arbeit basiert auf Deutschaufsätzen von finnischen Gymnasiasten. Es soll der Versuch unternommen werden, die Schreibkompetenz der Lernenden zu analysieren. Es wird betrachtet, welche Verbstrukturen sie in ihren Aufsätzen benutzen und wie sie sie anwenden. Auch die Stellung des Verbs wird untersucht. Diese werden mit den Inhalten, die im Unterricht gelehrt worden sind, verglichen. Das Beherrschen der Verbstrukturen hat einen starken Einfluss auf die Bestimmung der sprachlichen Kompetenz. Das Primärmaterial besteht aus zwölf Aufsätzen und neun Lehrbüchern. Die Aufsätze wurden während des dritten und sechsten B2-Deutschkurses geschrieben. Die SchülerInnen haben unterschiedliche Hintergründe: Einige lernen Deutsch erst seit ein Paar Jahren, während andere sogar deutsche Verwandte haben. Der deutsche Hintergrund hat die Sprachkompetenz positiv beeinflusst, aber auch z. B. die Motivation, sprachliche Begabung und die Lehrwerke tragen hierzu bei. Auch Transfer wurde untersucht. Die Aufsätze wurden Satz per Satz analysiert. Es wurde betrachtet, welche Strukturen vorkamen und wie vielseitig sie benutzt wurden. Die Lernenden hatten in den ersten Texten sehr einfache Sätze geschrieben, aber sie haben Variation z. B. durch die Inversion gebracht. In den näcshten Aufsätzen war der Satzbau komplexer, aber dadurch waren die Sätze manchmal nicht sofort zu verstehen. Der sprachliche Fortschritt wurde mit Hilfe des Analysemodells von Rösch (2011) analysiert und die Sprachkompetenz wurde in die Niveaus des gemeinsamen europäischen Referenzrahmens eingeordnet. Der finnische Lehrplan stellt eigene Ziele zum Fremdsprachenunterricht und die Lernenden dieser Untersuchungsgruppe haben die Ziele schon nach dem dritten Kurs erreicht. Zwischen dem dritten und sechsten Kurs haben sie das folgende Niveau nicht erreicht, aber die Schreibkompetenz hat sich vertieft. Mit ein bisschen Übung würden sie bestimmt das folgende Niveau erreichen. Die Kompetenzunterschiede zwischen den Lernenden waren deutlich zu sehen.

Diet, microbes and gut immune responses in the pathogenesis of experimental type 1 diabetes

Type 1diabetes (T1D) is an autoimmune disease, which is influenced by a variety of environmental factors including diet and microbes. These factors affect the homeostasis and the immune system of the gut. This thesis explored the altered regulation of the immune system and the development of diabetes in non-obese diabetic (NOD) mice. Inflammation in the entire intestine of diabetes-prone NOD mice was studied using a novel ex-vivo imaging system of reactive oxygen and nitrogen species (RONS), in relation to two feeding regimens. In parallel, gut barrier integrity and intestinal T-cell activation were assessed. Extra-intestinal manifestations of inflammation and decreased barrier integrity were sought for by studying peritoneal leukocytes. In addition, the role of pectin and xylan as dietary factors involved in diabetes development in NOD mice was explored. NOD mice showed expression of RONS especially in the distal small intestine, which coincided with T-cell activation and increased permeability to macromolecules. The introduction of a casein hydrolysate (hydrolysed milk protein) diet reduced these phenomena, altered the gut microbiota and reduced the incidence of T1D. Extra-intestinally, macrophages appeared in large numbers in the peritoneum of NOD mice after weaning. Peritoneal macrophages (PM) expressed high levels of interleukin-1 receptor associated kinase M (IRAK-M), which was indicative of exposure to ligands of toll-like receptor 4 (TLR-4) such as bacterial lipopolysaccharide (LPS). Intraperitoneal LPS injections activated T cells in the pancreatic lymph nodes (PaLN) and thus, therefore potentially could activate islet-specific T cells. Addition of pectin and xylan to an otherwise diabetes-retarding semisynthetic diet affected microbial colonization of newly-weaned NOD mice, disturbed gut homeostasis and promoted diabetes development. These results help us to understand how diet and microbiota impact the regulation of the gut immune system in a way that might promote T1D in NOD mice.