Gut Microbiota and Metabolic Disorders

Obesity and its co-morbidities, such as metabolic syndrome (MetS), non-alcoholic fatty liver disease (NAFLD) and type 2 diabetes, have increased over the last few decades like an epidemic. So far the mechanisms of many metabolic diseases are not known in detail and currently there are not enough effective means to prevent and treat them. Several recent studies have shown that the unbalanced gut microbiota composition (GMC) and activity have an influence on the fat accumulation in the body. Further, it seems that the GMC of obese individuals differs from the lean. The aim of this study was to investigate whether there are differences between the GMC of metabolically impaired overweight/obese (MetS group), metabolically healthy overweight/obese and normal-weight individuals. In addition, the mechanisms by which the gut bacteria as well as their specific structures, such as flagellin (FLG) that stimulates the Toll-like receptor 5 (TLR5) affect metabolism, were investigated both in vivo and in vitro in human adipocytes and hepatocytes. The results of this study show that the abundance of certain gram-positive bacteria belonging to the Clostridial cluster XIV was higher in the MetS group subjects compared to their metabolically healthy overweight/obese and lean counterparts. Metabolically impaired subjects tended to also have a greater abundance of potentionally inflammatory Enterobacteria in their gut and thus seemed to have aberrant GMC. In addition, it was found that subjects with a high hepatic fat content (HHFC group) had less Faecalibacterium prausnitzii in their gut than individuals with low hepatic fat content. Further gene expression analysis revealed that the HHFC group also had increased inflammation cascades in their adipose tissue. Additionally, metabolically impaired individuals displayed an increased expression of FLG-recognizing TLR5 in adipose tissue, and the TLR5 expression levels associated positively both with liver fat content and insulin resistance in humans. These changes in the adipose tissue may further contribute to the impaired metabolism observed, such as insulin resistance and dyslipidemia. In vitro -studies showed that the FLG-induced TLR5 activation in adipocytes enhanced the hepatic fat accumulation by decreasing insulin signaling and mitochondrial functions and increasing triglyceride synthesis due to increased glycerol secretion from adipocytes. In conclusion, the findings of this study suggest that it may be possible that the novel prevention and personalized treatment strategies based on GM modulation will succesfully be developed for obesity and metabolic disorders in the future.

The Formation Of Internationalization Strategies Within The Construction Industry:Vision and Strategy

Internationalization represents a complex topic that has been researched for quite some time. However, since it continues to be extremely current a topic, its significance has not diminished, but maybe even increased in importance. Companies today face extreme pressure to enter new markets in the hope of growing, becoming more profitable, increasing market share, attracting new customers and meeting the requirements of its share- and stakeholders. In the increasingly global business environment of today, companies are facing both challenges and possible advantages of internationalization. Few companies are not operating internationally and it is becoming the question of ‘Why not?’ rather than ‘Why?’ to internationalize business operations. Internationalization and the importance of strategy are discussed in this research from the viewpoint of three case companies that were interviewed about internationalization strategies. This research project is a qualitative study that answers the research question of How is a business strategy constructed for entering a new market? The sub-questions are • How are goals set and what indicators are used to monitor the achievement of these goals? • What are the key characteristics of a strategy implementation process? The research method chosen for this study is a multiple-case study. Three case companies were chosen for the interviews in order to gain in-depth data of internationalization strategies within the construction industry.

Työnantajabrandin vision suhde todellisuuteen

Kilpailu osaavasta työvoimasta on saanut yritykset panostamaan työnantajabrandiinsa. Työnantajabrandi yhtenä yrityksen brandipääoman osa-alueena auttaa yrityksiä erottumaan kilpailijoista ja siten houkuttelemaan yritykseen sopivia työntekijöitä sekä säilyttämään jo olemassa olevat työntekijät. Työnantajabrandi ei aina näyttäydy yrityksen työntekijöille tai –hakijoille niin kuin yritys sen haluaisi näyttäytyvän. Henkilöstöhallinnon ja johdon visio saattaa siis olla ristiriidassa sen kanssa, miten brandi todellisuudessa koetaan. Mikäli yritys ei pysty viestittämään oikeanlaista brandi-imagoa tai säilyttämään brandilupauksiaan vaikuttaa tämä yrityksen työntekijöiden sitoutumiseen ja tyytyväisyyteen negatiivisesti. Lisäksi yritykseen hakeutuu töihin yrityksen arvomaailmaan sopimattomia työntekijöitä. Tutkimuksen tarkoituksena on selvittää työnantajabrandin vision suhdetta todellisuuteen case-yrityksen kautta. Case-yritykseksi on valittu kansainvälinen konsultointiyritys, jolle osaava työvoima on elinehto. Tutkimusmenetelmäksi valittiin puolistrukturoidut haastattelut eli aihetta on lähestytty laadullisen menetelmän kautta. Tutkimukseen haastateltiin vision selvittämiseksi case-yrityksen henkilöstöhallintoa sekä perehdyttiin olemassa olevaan materiaaliin yrityksen brandista. Vision toteutumisen selvittämiseksi haastateltiin 19 työntekijää yrityksen kahdesta eri liiketoimintayksiköstä. Teorian pohjalta tarkentunut teoreettinen viitekehys pitää sisällään yrityksen psykologisen sopimuksen, sisäisen ja ulkoisen brandiviestinnän sekä organisaatiokulttuurin. Haastattelut rakennettiin näiden teemojen ympärille. Empiirisen osuuden tulokset vahvistavat teoreettista viitekehystä, jonka mukaan psykologisella sopimuksella, brandiviestinnällä ja organisaatiokulttuurilla on vaikutusta siihen, miten työnantajabrandi koetaan yrityksen työntekijöiden keskuudessa. Organisaatiokulttuurin ja sitä ilmentävän brandipersoonallisuuden osalta korostuu yrityksen työntekijöiden sopivuus ja yhtäläisyys yrityksen organisaatiokulttuuriin voimistamaan haluttua brandimielikuvaa. Psykologisen sopimuksen osalta empiria tukee todenmukaisen kuvan luomisen merkitystä yrityksen brandiviestinnässä. Lisäksi psykologisen sopimuksen osalta sosiaaliset edut nähdään tärkeänä tekijänä työnantajassa. Ihmisistä välittämisellä ja työilmapiirillä voidaan nähdä olevan suuri merkitys työntekijöiden työssä viihtymiselle. Työntekijät myös odottavat nykypäivänä nopeaa urakehitystä ja koulutukseen panostamista uraltaan. Lisäksi joustavuus ja vapaa-ajan ja työn tasapainottaminen koetaan tärkeiksi. Haastattelut tukevat myös teoreettisen viitekehyksen oletusta, että yrityksen visio työnantajabrandista eroaa usein siitä kuvasta, joka työntekijöille välittyy

Adherence to Treatment in Psychotic Disorders - Development of user-centered mobile health intervention

The aim of this study was to explore adherence to treatment among people with psychotic disorders through the development of user-centered mobile technology (mHealth) intervention. More specifically, this study investigates treatment adherence as well as mHealth intervention and the factors related to its possible usability. The data were collected from 2010 to 2013. First, patients’ and professionals’ perceptions of adherence management and restrictive factors of adherence were described (n = 61). Second, objectives and methods of the intervention were defined based on focus group interviews and previously used methods. Third, views of patients and professionals about barriers and requirements of the intervention were described (n = 61). Fourth, mHealth intervention was evaluated based on a literature review (n = 2) and patients preferences regarding the intervention (n = 562). Adherence management required support in everyday activities, social networks and maintaining a positive outlook. The factors restricting adherence were related to illness, behavior and the environment. The objective of the intervention was to support the intention to follow the treatment guidelines and recommendations with mHealth technology. The barriers and requirements for the use of the mHealth were related to technology, organizational issues and the users themselves. During the course of the intervention, 33 (6%) out of 562 participants wanted to edit the content, timing or amount of the mHealth tool, and 23 (4%) quit the intervention or study before its conclusion. According to the review, mHealth interventions were ineffective in promoting adherence. Prior to the intervention, participants perceived that adherence could be supported, and the use of mHealth as a part of treatment was seen as an acceptable and efficient method for doing so. In conclusion, the use of mHealth may be feasible among people with psychotic disorders. However, clear evidence for its effectiveness in regards to adherence is still currently inconclusive.

Design and implementation of machine vision system for the mobile assembly robot

The review of intelligent machines shows that the demand for new ways of helping people in perception of the real world is becoming higher and higher every year. This thesis provides information about design and implementation of machine vision for mobile assembly robot. The work has been done as a part of LUT project in Laboratory of Intelligent Machines. The aim of this work is to create a working vision system. The qualitative and quantitative research were done to complete this task. In the first part, the author presents the theoretical background of such things as digital camera work principles, wireless transmission basics, creation of live stream, methods used for pattern recognition. Formulas, dependencies and previous research related to the topic are shown. In the second part, the equipment used for the project is described. There is information about the brands, models, capabilities and also requirements needed for implementation. Although, the author gives a description of LabVIEW software, its add-ons and OpenCV which are used in the project. Furthermore, one can find results in further section of considered thesis. They mainly represented by screenshots from cameras, working station and photos of the system. The key result of this thesis is vision system created for the needs of mobile assembly robot. Therefore, it is possible to see graphically what was done on examples. Future research in this field includes optimization of the pattern recognition algorithm. This will give less response time for recognizing objects. Presented by author system can be used also for further activities which include artificial intelligence usage.